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N-Acetylneuraminic Acid Aldolase [EC 4.1.3.3]

Sialic Acid Related Products

Origin

Escherichia coli

Reaction

N-Acetylneuraminate N-Acetyl-D-mannosamine + Pyruvate

Appearance

White amorphous powder.

Activity

More than 30 units/mg protein.

Unit definition

One unit is the amount of enzyme required to liberates 1 µmol of N-Acetylmannosamine (or Pyruvic Acid) per minute at pH 7.7 at 37°C, using N-Acetylneuraminic Acid (NANA) as a substrate.

Storage

Stable for one year when stored below 5°C. For prolonged storage, keep at -20°C.

Contaminant

Free from NADH oxidase

Properties

Molecular weight --- Approx. 98,000 Da (gel filtration)
Optimum pH --- 7.5 ~ 8.0
pH stability --- 6.0 ~ 9.0
Thermal stability --- below 65°C (pH 7.0, 20 min)
Substrate specificity --- N-Glycolylneuraminic Acid (NGNA) is cleaved as well as NANA.
Km = 3.6 mM (NANA), 4.3 mM (NGNA)

Uses

Enzymatic determination of Sialic Acid and enzymatic syntheses of novel Sialic Acid derivatives.

Ordering Information

Sialic Acid Related Products - Enzymes
Product Cat.No. Storage Quantity Price(US$)  
N-Acetylneuraminic acid aldolase N-Acetylneuraminate pyruvate lyase [EC 4.1.3.3] 00628-84 -20°C 10 units 65.00 Buy