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COSMOSIL HILIC

Others / Hydrophilic interaction

COSMOSIL HILIC is a new column for hydrophilic interaction chromatography with Triazole bonded silica packing material. The hydrophilic interaction chromatography is a variation of normal phase chromatography where a polar stationary phase is used with a mobile phase which contains a high concentration of organic solvent and a low concentration of aqueous eluent. The main retention mechanism is the partitioning of the polar analytes between the polar stationary and the non-polar mobile phase. As it is also called "aqueous normal phase", the elution order is similar to that of normal phase and the sample elution is in the order of increasing hydrophilicity. Without using ion-pair reagent COSMOSIL HILIC retains highly polar analytes that would not be retained in reversed phase chromatography. It also shows a weak anion-exchange mechanism with the positively charged stationary phase, thus acidic compound is strongly retained.

COSMOSIL HILIC
Silica gel high purity porous spherical silica gel
Average particle size 2.5 µm 5 µm
Average pore size approx. 130Å approx. 120Á
Specific surface area approx. 330m²/g approx. 300m²/g
Stationary phase Triazole Triazole
Main interaction Hydrophilic interaction Hydrophilic interaction

 

Comparison with C18 column

COSMOSIL HILIC can separate Glycine and Glycylglycine without ion-pair reagent. Although C18 column can separate them with ion-pair reagents, there are some disadvantages such as column equilibration, preparation of mobile phase and column deterioration.

Different interactions

The retention Mechanism of COSMOSIL HILIC is the combination of hydrophilic interaction and anion exchange, and the retention can be controlled by changing the mobile phase.

Application

Analysis data

COSMOSIL Application Data
LC/MS/MS (COSMOSIL HILIC 2.0mm I.D. x 150mm)

Combination with C18 columns

Selection of mobile phase

Following are the recommended mobile phases for different samples.
Acetonitrile / Water = 90 / 10
Acetonitrile / 10mmol/l CH3COONH4 = 90 / 10
Acetonitrile / 10mmol/l CH3COONH4 = 70 / 30
Acetonitrile / 10mmol/l CH3COONH4 = 50 / 50
(not eluted) → Acetonitrile / 10mmol/l Phosphatase buffer (pH7.0) = 50 / 50

Poster Presentation

HPLC 2008
May 13-15, 2008
Baltimore, MD

piNAP
COSMOSIL HILIC (PDF)

HPLC 2007
June 17-21, 2007
Ghent, Belgium

COSMOSIL HILIC
COSMOSIL HILIC (PDF)

COSMOSIL HILIC Application Notebook

COSMOSIL HILIC Application Notebook contains about 200 chromatograms for the separation of polar compounds using COSMOSIL HILIC column. It also describes how the mobile phase conditions, such as buffer pH and salt concentration influence the separation in HILIC mode.

COSMOSIL HILIC Application Notebook
COSMOSIL HILIC Application Notebook (PDF) : 18.5 MB

Ordering Information

Analytical Column (Particle Size : 2.5 µm)
Product Cat.No. Size End-Fitting Quantity Price(US$)  
COSMOSIL 2.5HILIC Packed Column, 2.0mmI.D. x 50mm 11766-21 2.0mmI.D. x 50mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 2.0mmI.D. x 75mm 11768-01 2.0mmI.D. x 75mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 2.0mmI.D. x 100mm 11769-91 2.0mmI.D. x 100mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 2.0mmI.D. x 150mm 11770-51 2.0mmI.D. x 150mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 3.0mmI.D. x 50mm 11771-41 3.0mmI.D. x 50mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 3.0mmI.D. x 75mm 11772-31 3.0mmI.D. x 75mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 3.0mmI.D. x 100mm 11773-21 3.0mmI.D. x 100mm Waters type 1 pkg 710.00 Buy
COSMOSIL 2.5HILIC Packed Column, 3.0mmI.D. x 150mm 11774-11 3.0mmI.D. x 150mm Waters type 1 pkg 710.00 Buy
Analytical Column (Particle Size : 5 µm)
Product Cat.No. Size End-Fitting Quantity Price(US$)  
COSMOSIL HILIC Packed Column, 1.0mml.D. x 150mm 07869-11 1.0mml.D. x 150mm Waters type 1 pkg 420.00 Buy
COSMOSIL HILIC Packed Column, 1.0mml.D. x 250mm 07870-71 1.0mml.D. x 250mm Waters type 1 pkg 490.00 Buy
COSMOSIL HILIC Packed Column, 2.0mml.D.x 30mm 08568-21 2.0mml.D.x 30mm Waters type 1 pkg 420.00 Buy
COSMOSIL HILIC Packed Column, 2.0mml.D.x 50mm 07052-91 2.0mml.D.x 50mm Waters type 1 pkg 500.00 Buy
COSMOSIL HILIC Packed Column, 2.0mml.D.x 100mm 08569-11 2.0mml.D.x 100mm Waters type 1 pkg 420.00 Buy
COSMOSIL HILIC Packed Column, 2.0mml.D.x 150mm 07054-71 2.0mml.D.x 150mm Waters type 1 pkg 420.00 Buy
COSMOSIL HILIC Packed Column, 2.0mml.D.x 250mm 07489-91 2.0mml.D.x 250mm Waters type 1 pkg 490.00 Buy
COSMOSIL HILIC Packed Column, 3.0mml.D. x 150mm 07871-61 3.0mml.D. x 150mm Waters type 1 pkg 450.00 Buy
COSMOSIL HILIC Packed Column, 3.0mml.D. x 250mm 07872-51 3.0mml.D. x 250mm Waters type 1 pkg 490.00 Buy
COSMOSIL HILIC Packed Column, 4.6mml.D.x 50mm 07973-31 4.6mml.D.x 50mm Waters type 1 pkg 440.00 Buy
COSMOSIL HILIC Packed Column, 4.6mml.D.x 150mm 07056-51 4.6mml.D.x 150mm Waters type 1 pkg 420.00 Buy
COSMOSIL HILIC Packed Column (3 Lots Set), 4.6mml.D.x 150mm 09385-23 4.6mml.D.x 150mm Waters type 3 pkg 1,260.00 Buy
COSMOSIL HILIC Packed Column, 4.6mml.D.x 250mm 07057-41 4.6mml.D.x 250mm Waters type 1 pkg 490.00 Buy
Guard Column (Particle Size : 5 µm)
Product Cat.No. Size End-Fitting Quantity Price(US$)  
COSMOSIL HILIC Guard Column, 2.0mmI.D.x 10mm 07569-41 2.0mmI.D.x 10mm Waters type 1 pkg 350.00 Buy
COSMOSIL HILIC Guard Column, 4.6mml.D.x 10mm 07055-61 4.6mml.D.x 10mm Waters type 1 pkg 250.00 Buy
COSMOSIL HILIC Guard Column, 10mml.D.x 20mm 07058-31 10mml.D.x 20mm Waters type 1 pkg 440.00 Buy
COSMOSIL HILIC Guard Column, 20mml.D. x 20mm 07854-91 20mml.D. x 20mm Waters type 1 pkg 950.00 Buy
COSMOSIL HILIC Guard Column, 20mml.D. x 50mm 07873-41 20mml.D. x 50mm Waters type 1 pkg 1,310.00 Buy
COSMOSIL HILIC Guard Column, 28mml.D. x 50mm 07874-31 28mml.D. x 50mm Waters type 1 pkg 2,890.00 Buy
Preparative Column (Particle Size : 5 µm)
Product Cat.No. Size End-Fitting Quantity Price(US$)  
COSMOSIL HILIC Packed Column, 10mml.D.x 150mm 05564-51 10mml.D.x 150mm Waters type 1 pkg 990.00 Buy
COSMOSIL HILIC Packed Column, 10mml.D.x 250mm 07059-21 10mml.D.x 250mm Waters type 1 pkg 1,200.00 Buy
COSMOSIL HILIC Packed Column, 20mml.D.x 250mm 07060-81 20mml.D.x 250mm Waters type 1 pkg 2,630.00 Buy
COSMOSIL HILIC Packed Column, 28mml.D. x 250mm 07875-21 28mml.D. x 250mm Waters type 1 pkg 5,010.00 Buy