Nacalai USA - Innovations for Life Sciences

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Recombinant Human LIF

Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Recombinant Human Leukemia inhibitory factor (hLIF) is produced in E. coli. It contains a single non-glycosylated polypeptide chain 181 amino acids and has a molecular mass of 20kDa. hLIF is purified by proprietary techniques using HPLC and FPLC chromatography.

SDS PAGE of hLIF Sample

Source

Escherichia Coli.

Purity

Greater than 95%, as determined by SDS-PAGE (See Fig. 1)

Endotoxin

Less than 0.01 ng/µg cytokine as determined by the LAL assay

Bioactivity

The activity of human LIF was tested by the M1 cell differentiation assay. Specific activity of the sample is approximate 108 units/mg. 0.5x107 units, identical 50 µg of pure protein, are sufficient to treat 5.0 L of stem cells including human embryonic stem cells, neural stem cells, hematopoietic stem cells, mesenchymal stem cells and induced pluripotent stem cells.

Formulation

0.22 µm filtered sterile liquid, phosphate-buffered saline with 0.02% Tween-20 and 1% BSA.

Storage

Maintain at 4°C for 18 months. Freeze-thaw should be avoided as it results in loss of activity.

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Recombinant Human LIF NU0013-1 4°C 1 ml, 106 units/ml 250.00 Buy
Recombinant Human LIF NU0013-2 4°C 1 ml, 0.5 x 107 units/ml 650.00 Buy