Nacalai USA - Innovations for Life Sciences

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DNA Ladder

Agarose gel electrophoresis

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes, suitable for use as molecular weight standards for agarose gel electrophoresis. We offer three types of DNA Ladders that are pre-mixed, ready-to-use molecular weight markers containing tracking dye.

100 bp DNA Ladder (Ready-to-Use)

The DNA includes 12 fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 µg a load) for approximating the mass DNA in comparably intense sample of similar size.

Range: 100-3,000 bp
Number of bands: 12
Concentration: 108 µg/ml
Package size: 54 µg/500 µl
Recommended load: 5 µl/well
Containing Orange G and Xylene Cyanol FF as tracking dyes.
Source: PCR products and double-stranded DNA digested with appropriate restriction enzyme, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Storage: Store at 25°C for 6 months
Store at 4°C for 12 months
Store at -20°C for 24 months

1 kb DNA Ladder (Ready-to-Use)

The DNA includes 13 fragments ranging from 250-10,000 base pairs. The 1,000 and 3,000 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 µg a load) for approximating the mass DNA in comparably intense sample of similar size.

Range: 250-10,000 bp
Number of bands: 13
Concentration: 100 µg/ml
Package size: 50 µg/500 µl
Recommended load: 5 µl/well
Containing Bromophenol Blue and Xylene Cyanol FF as tracking dyes.
Source: PCR products and double-stranded DNA digested with appropriate restriction enzyme, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Storage: Store at 25°C for 6 months
Store at 4°C for 12 months
Store at -20°C for 24 months

1 kb Plus DNA Ladder (Ready-to-Use)

The DNA includes 19 fragments ranging from 100-10,000 base pairs. The 500, 1,500 and 3,000 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.86 µg a load) for approximating the mass DNA in comparably intense sample of similar size.

Range: 100-10,000 bp
Number of bands: 19
Concentration: 172 µg/ml
Package size: 86 µg/500 µl
Recommended load: 5 µl/well
Containing Orange G, Xylene Cyanol FF and Bromophenol Blue as tracking dyes.
Source: PCR products and double-stranded DNA digested with appropriate restriction enzyme, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Storage: Store at 25°C for 6 months
Store at 4°C for 12 months
Store at -20°C for 24 months

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
100 bp DNA Ladder (Ready-to-Use) NU02001 4°C 500 µl 50.00 Buy
1 kb DNA Ladder (Ready-to-Use) NU02002 4°C 500 µl 50.00 Buy
1 kb Plus DNA Ladder (Ready-to-Use) NU02003 4°C 500 µl 80.00 Buy