Nacalai USA - Innovations for Life Sciences

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DNA Ladder

Agarose gel electrophoresis

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes, suitable for use as molecular weight standards for agarose gel electrophoresis. We offer two types of DNA Ladders that are pre-mixed, ready-to-use molecular weight markers containing tracking dyes.

100 bp DNA Ladder (Ready-to-Use)

The DNA includes 12 fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 µg a load) for approximating the mass DNA in comparably intense sample of similar size.

Range: 100 - 3,000 bp
Number of bands: 12
Concentration: 108 µg / ml
Package size: 54 µg / 500 µl
Recommended load: 5 µl / well
Containing Orange G and Xylene Cyanol FF as tracking dyes.
Source: PCR products and double-stranded DNA digested with appropriate restriction enzyme, are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Storage: Store at 25°C for 6 months
Store at 4°C for 12 months
Store at -20°C for 24 months

1 kb DNA Ladder (Ready-to-Use)

The DNA includes 13 fragments ranging from 100-10,000 base pairs. The 1,000 and 3,000 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 µg a load) for approximating the mass DNA in comparably intense sample of similar size.

Range: 100-10,000 bp
Number of bands: 13
Concentration: 100 µg / ml
Package size: 50 µg / 500 µl
Recommended load: 5 µl / well
Containing Bromophenol Blue and Xylene Cyanol FF as tracking dyes.
Source: PCR products and double-stranded DNA digested with appropriate restriction enzyme are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Storage: Store at 25°C for 6 months
Store at 4°C for 12 months
Store at -20°C for 24 months

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
100 bp DNA Ladder (Ready-to-Use) NU02001 4°C 500 µl 50.00 Buy
1 kb DNA Ladder (Ready-to-Use) NU02002 4°C 500 µl 50.00 Buy