Prepare concentrated stock solution of rHSA by dissolving in cell culture grade PBS or water. Use gentle mixing or inversion to minimize the formation of bubbles. Allow rHSA to dissolve for at least 1 hr. Liquid stock should be sterile filtered prior to use. We recommends a pre-filtration step with a 0.8 um filter prior to a final sterile filtration step with a 0.45 µm or 0.2 µm filter.
We recommend the adaptation of cells to rHSA supplemented medium for a couple of passages to insure optimum performance. Following the adaptation, the end user can determine the optimum concentration of rHSA for their particular purpose. Cells previously adapted to a medium containing albumin typically require little adaptation to rHSA.