Nacalai USA - Innovations for Life Sciences

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Bullet PAGE One Precast Gel

Ultra-Fast Protein Electrophoresis in 10 min! / Precast Gel: Gradient and Single Parcentage gels are available

Features

  • Only 10 minutes with 400 V
  • High transfer efficiency of proteins on western blot membrane
  • Works well in conventional Laemmli running buffer and sample buffer
  • 17-well gel is usable with multichannel pipet for consistent loading

 

» Bullet PAGE One Precast Gel vs Laemmli gel 16x
(YouTube) YouTube

Bullet PAGE One Precast Gel vs Laemmli gel 16x

 

 

  • Only 10 minutes with 400 V
  • High transfer efficiency of proteins on western blot membrane
  • Works well in conventional Laemmli running buffer and sample buffer
  • 17-well gel is usable with multichannel pipet for consistent loading

» Bullet PAGE One Precast Gel vs Laemmli gel 16x
(YouTube) YouTube

 

 

 

  • Only 10 minutes with 400 V
  • High transfer efficiency of proteins on western blot membrane
  • Works well in conventional Laemmli running buffer and sample buffer
  • 17-well gel is usable with multichannel pipet for consistent loading

» Bullet PAGE One Precast Gel vs Laemmli gel 16x
(YouTube) YouTube

 

 

 

 

Performance Comparison

Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.

Product Name Bullet PAGE One Company A Company B
Gel % 5 - 15% 4 - 15% 4 - 12%
Product No. 13079-84 - -
Electrophoresis Time 10 min. 33 min. 50 min.
Electrophoresis Images

Electrophoresis condition

Sample: Protein Markers(10x)(Prod No. 29458-24), 5 µl
Gel Staining: CBB Stain One (Prod No. 04543)
Voltage Constant: Bullet PAGE One: 400V, Company A and Company B: 200V

Comparison of Protein Transfer Efficiency

Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A's, even though blotting time was only 10 min.

              

Sample
① Protein Ladder One(Triple-color)(Product No. : 09547-74) 
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added

Transfer
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)

PVDF membrane staining
Stain PVDF membrane with CBB Stain One (Product No. : 04543).

Gel Types

Gel Gradient Gel Single Percentage Gel
Gel % 5 - 11% 5 - 15% 6% 8%
Product No. 13 well
17 well 
13077-04
13078-94
13079-84
13080-44
13081-34
13082-24
13083-14
13084-04
Electrophoresis Images

Product Specification

Glass Plate Size: W100mm x H80mm x T3.2mm
Gel Size: W80mm x H60mm x T1.0mm
 Sample Well Configuration / Recommended Load Volume: 13-well / < 20 µl, 17-well / < 14 µl  

Compatible for Gel Staining and Western Blotting

Coomassie brilliant blue staining and western blotting

The western blot is a composite image using different exposure times for each protein. This data shows that Bullet PAGE One Precast Gel is compatible with CBB staining and western blot techniques.

Data courtesy of professor Tatsuya Moriyama, Faculty of Agriculture, Kinki University

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Bullet PAGE One Precast Gel (5-11%, 13 wells) 13077-04 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (5-11%, 17 wells) 13078-94 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (5-15%, 13 wells) 13079-84 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (5-15%, 17 wells) 13080-44 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (6%, 13 wells) 13081-34 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (6%, 17 wells) 13082-24 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (8%, 13 wells) 13083-14 4-10°C 10 sheets 200.00 Buy
Bullet PAGE One Precast Gel (8%, 17 wells) 13084-04 4-10°C 10 sheets 200.00 Buy