FG beads - Linker (Epoxy) beads
Compounds with phenol groups are immobilized easily
It is difficult for the OH groups to immobilize it to beads in reactivity being low, but phenol groups can be immobilized on epoxy beads under an alkaline condition because reactivity is high. Because epoxy beads produce almost no non-specific protein adsorption, it is not necessary to mask the remaining epoxy groups.
Product information
| Storage temperature | 4℃ (2-8℃), Don't freeze, protected from light |
| Storage buffer | ultrapure water |
| Magnetization | superparamagnetism(>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Epoxy groups |
| Amounts of the functional groups | 200-300 nmol/mg |
Protocol
Chemical pulldown
| E001 | Screening by using ligand immobilized beads | 259KB |
|---|---|---|
| E003 | Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads | 111KB |
| E004 | Immobilization of ligands (carboxylic compounds) on OH beads | 78KB |
| E005 | Immobilization of ligands (carboxylic compounds) on NH2 beads | 97KB |
| E008 | Immobilization of ligands (compounds with NH2 groups) on COOH beads | 139KB |
| E012 | Competitive inhibition | 261KB |
| E013 | Drug elution | 260KB |
| E014 | Immobilization of ligands (compounds with NH2 groups) on NHS beads | 75KB |
| E015 | Immobilization of MTX aminated derivatives on NHS beads | 66KB |
| E108 | Immobilization of Ligand on Streptavidin beads | 102KB |
| E109 | Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction | 65KB |
| E201 | Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) | 1,329KB |
The protocols above are for immobilization of 2.5 mg of a ligand. For smaller scale, click the link here to get protocols.
Immunoprecipitation, Protein-protein interaction
| E011 | Immunoprecipitation | 77KB |
|---|---|---|
| E101 | Immobilization of proteins on COOH beads | 83KB |
| E102 | Immobilization of His-Tag proteins on Ts beads | 89KB |
| E105 | Immobilization of Antibodies or Proteins on NHS beads | 23KB |
| E106 | Immobilization of Antibodies or Proteins on Epoxy Beads | 65KB |
| E107 | Direct Quantification of Immobilized Proteins (Antibodies) | 107KB |
| E108 | Immobilization of Ligand on Streptavidin beads | 102KB |
| E110 | Immobilization of antibodies on Protein A beads and Protein G beads | 103KB |
Purification of DNA and RNA binding substances
| E001 | Screening by using ligand immobilized beads | 259KB |
|---|---|---|
| E301 | Immobilization of double strand DNA on Plain beads | 143KB |
Ordering Information
FG beads
| Product | Cat.No. | Storage | Quantity | Price(US$) | |
|---|---|---|---|---|---|
| Linker beads (Epoxy beads) | TAS8848N1110 | 4°C | 5 mg | 155.00 | Buy |