FG beads - Azide / Alkyne beads
The immobilization of the ligand by the click chemistry
Compounds or proteins with azide or alkyne groups are immobilized on FG azide or alkyne beads easily by using a click chemistry reaction.
Product information
| Storage temperature | 4℃ (2-8℃), Don't freezet |
| Storage buffer | ultrapure water |
| Magnetization | superparamagnetism(>10 emu/g) |
| Size of beads | 180 nm±30 nm |
| Concentration | 20 mg/ml |
| Functional groups | Azide groups |
| Amounts of the functional groups | 70-120 nmol/mg |
Application; Target fishing
Affinity purification of target protein, DHRF (Dihydrofolate reductase), of MTX (methotrexate) with FG Azide and Alkyne beads.
1
Protocol
Chemical pulldown
| E001 | Screening by using ligand immobilized beads | 259KB |
|---|---|---|
| E003 | Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads | 111KB |
| E004 | Immobilization of ligands (carboxylic compounds) on OH beads | 78KB |
| E005 | Immobilization of ligands (carboxylic compounds) on NH2 beads | 97KB |
| E008 | Immobilization of ligands (compounds with NH2 groups) on COOH beads | 139KB |
| E012 | Competitive inhibition | 261KB |
| E013 | Drug elution | 260KB |
| E014 | Immobilization of ligands (compounds with NH2 groups) on NHS beads | 75KB |
| E015 | Immobilization of MTX aminated derivatives on NHS beads | 66KB |
| E108 | Immobilization of Ligand on Streptavidin beads | 102KB |
| E109 | Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction | 65KB |
| E201 | Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) | 1,329KB |
The protocols above are for immobilization of 2.5 mg of a ligand. For smaller scale, click the link here to get protocols.
Immunoprecipitation, Protein-protein interaction
| E011 | Immunoprecipitation | 77KB |
|---|---|---|
| E101 | Immobilization of proteins on COOH beads | 83KB |
| E102 | Immobilization of His-Tag proteins on Ts beads | 89KB |
| E105 | Immobilization of Antibodies or Proteins on NHS beads | 23KB |
| E106 | Immobilization of Antibodies or Proteins on Epoxy Beads | 65KB |
| E107 | Direct Quantification of Immobilized Proteins (Antibodies) | 107KB |
| E108 | Immobilization of Ligand on Streptavidin beads | 102KB |
| E110 | Immobilization of antibodies on Protein A beads and Protein G beads | 103KB |
Purification of DNA and RNA binding substances
| E001 | Screening by using ligand immobilized beads | 259KB |
|---|---|---|
| E301 | Immobilization of double strand DNA on Plain beads | 143KB |
| E001 | Screening by using ligand immobilized beads | 259KB |
|---|---|---|
| E301 | Immobilization of double strand DNA on Plain beads | 143KB |