Biotinylated kinases are now available from Carna Biosciences. These important reagents are best suited for the study of binding affinity and other kinase-molecule interactions on devices measuring Surface Plasmon Resonance (SPR), BioLayer Interferometry (BLI) and other similar values. They can also be utilized in homogenous proximity-based binding assays such as TR-FRET to interrogate inhibitor binding affinity, determine on-off rates, and measure binding kinetics.

The immobilization of target proteins onto sensor surfaces without impairing their structure and activity can be quite a challenging step in small molecule drug discovery.
Carna' s in-house single-site specifically biotinylated kinases can easily facilitate this immobilization and lead to the acquisition of real-time and accurate data for quick evaluation in your programs.

Advantages of Carna’s Biological Biotinylation

• Kinases are labeled with a single biotin on the N-terminus
• Easy-to-use - no additional labeling required
• Native, catalytically active kinase domains
• High quality human proteins produced in Baculovirus expression system (insect cells)
• Stable Activity
• Some kinases are available with pre-activation by ATP treatment, or unactivated (no ATP pre-treatment)

Advantages vs. Chemical Biotinylation

• Single vs. multiple biotin molecules, which can possibly interfere with the ATP binding site following chemical biotinylation.
• Preserved kinase activity and structure, which can be altered with chemical modification.
• No additional purification is required (for excluding non-reacted biotin).