Nacalai USA - Innovations for Life Sciences

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About SunShell (Core-shell particle)

SunShell column has not only an inert surface but also high stability due to deactivated silanol groups. SunShell 2.6 μm column can be used on both HPLC and UHPLC because it shows 2 times lower back presure than sub 2 μm column.

SunShell C18 2.6 μm sized 100x4.6mm has the same performance as a totally porous C18 5 μm sized 250x4.6mm, so that the same separation can be achieved by both columns without changing the separation condition consequently analysis time reduces one third.


Features of SunShell with 2 μm, 2.6 μm, 3.4 μm and 5 μm

  • 1.2 μm, 1.6 μm, 3.0 μm and 3.4 μm pf core diameter and 0.4 μm, 0.5 μm, 0.2 μm and 0.6 μm of superficially porous silica layer thickness.
  • Higher Efficency and higher throughpit  to compare with totally porous silica with same size.
  • Good peak shape for all compounds such as basic, acidic and chelating compounds.
  • High stability (pH range for SunShell C18: 1.5 to 10)
  • Low breeding

Comparison of theoretical plate and back pressure

Back pressure and theoretical plate were compared for 2 μm and sun 2 μm C18 and 2.6 μm SunShell C18. All columns showed almost the same theoretical plate except for brand A C18 1.9 μm.
However back pressure was not same. Especially Brand C C18 1.7 μm showed the highest back pressure. And SunShell C18 2.6 μm showed the lowest back pressure. On the comparison of theoretical plate per back pressure, SunShell indicated the largest value. This is a big advantage.
SunShell C18 2.6 μm 100 x 4.6 mm showed the same performance as conventional C18 5 μm 250 x 4.6 mm using HPLC.
An analysis time reduces to one third by only changing from conventional C18 column to SunShell C18 column and no changing the other conditions.
SunShell C18 showed 20% higher performance using UHPC than using HPLC.
Column Brand F C18, 5 µm, 250 x 4.6 mmSunShell C18, 2.6 µm, 100 x 4.6 mm
Mobile Phase CH3CN/20mM Phosphoric acid = 45/55
Flow Rate 1.0 ml/min1.8 ml/min at the lowest chromatogram
Temperature 25 oC
Detection UV 230 nm
Sample 1. Benzydamine2. Ketoprofen3. Naproxen4. Indomethacin5. Ibuprofen
Hitachi LaChrom ELITE (tube 0.25mmI.D.)
Jasco X-LC

Influence of cell capacity

To reduce total system volume of the HPLC using a semi-micro flow cell and 0.1 mm i.d. tubing, efficiency of SunShell C18 5 μm column improved to close true performance. Furthermore, decreasing the response speed and the short sampling time contribute high efficiency.

Column SunShell C18, 5 µm, 250 x 4.6 mm
Mobile Phase CH3CN/H2O=70/30
Flow Rate 1.0 ml/min
Temperature 40 oC
Detection UV 250 nm
1. UBenzy
2. Toluene
3. Acenaphthene
4. Butylbenzene
Hitachi LaChrom ELITE

Influence of the pipe inner diameter

The relationship between the inner diameter of a tubing and theoretical plate was evaluated.

When the tubing with a large inner diameter was used, the dead volume in the tubing was large.

The larger the dead volume, the broader the peak. The tubing with 0.1 mm or less than 0.1 mm of inner diameter is recommended especially when 2.1 mm i.d. column is used.

5 μm core shell column sized 4.6 mm i.d. is acceptable for HPLC or semi-micro HPLC. However, UH`LC is recommended for a 2.1 mm i.d. column because of a low column volume.

Column SunShell C18, 2.6 µm, 50 x 2.1 mm
Mobile Phase CH3CN/H2O=60/40
Flow Rate 0.3 ml/min
Temperature Ambient
Tube length 30 cm (Peek, from the column to the flow cell)
Instument X-LC (Jasco)
Response time 0.01 sec

Influence of response time

Response time of the detector is also important, when the peak width (4δ) is equal to or less than 1 second (above uracil) will need the following response time 0.03 seconds. Be about the peak width is 6 seconds, response time is desirable less than 0.3 seconds.

The same applies to further integrators of sampling rate, it is desirable to set the following 0.1 seconds.

Column SunShell C18, 2.6 µm, 100 x 4.6 mm
Mobile Phase CH3CN/H2O=60/40
Flow Rate 1.8 ml/min
Temperature Ambient
Sample Toluene
Tube i.d. 0.1mm x 20 cm Peeksil
Instrument X-LC (JASCO)

Excellent End-Capping

Hexamethyltri siloxane is used as an end-capping reagent. This reagent can stretch and be bent like a Geometrid caterpillar as shown in the figure, so that a functional group on the tip of the reagent can bond with a silanol group which is located anywhere.

This end-capping made influence of residual silanol groups the lowest. Then not only good peak shape of a basic compound but also excellent stability was achieved by this unique end-capping.