Nacalai USA - Innovations for Life Sciences

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About Sunniest

Developed a new bonded phase made with unique end capping!

This special C18 reagent consists of C18 chain part and endcapping part like an arm. An arm of this reagent moves like a Geometrid caterpillar, so that a functional group on the tip of the arm can bond with a silanol group which is located anywhere.

After bonding this unique C18 reagent an end-capping is densely done with trimethysilane (TMS) using SAC technique.(1)

 

(1)SAC technique is developed by Chromanik Technologies Inc. for Sunrise C18-SAC is an operation technique that silanol groups are converted into asiloxane bond under high temperature conditions.

 

Evaluation of the end-capping

Column Size
4.6 mmI.D. x 150 mm
Particle size 5 µm
Mobile Phase
CH3CN/20mM Phosphate buffer pH7.0 = 60/40
Flow Rate
1.0 ml/min
Temperature
40 oC
Sample
1. Uracil
2. Propranolol
3. Nortriptyline
4. Amitriptyline

Amitriptyline peak becomes a tailing peak by influence of residual silanol groups and many HPLC manufacturers use amitriptyline as an index of the amount of silanol groups. We evaluated the amount of residual using amitriptyline.

It is generally known that a peak tailing for a basic compound occurs more using acetonitrile in the mobile phase than using methanol although methanol has been used as an organic solvent in the mobile phase for separating basic compounds.

Sunniest C18, RP-AQUA, C8 showed sharp peaks without tailing even with acetonitrile. In other words, the Sunniest C18, RP-AQUA, C8 column, without the use of organic solvent is limited, can be set to a wide range of mobile phase conditions, you can construct an optimum separation conditions for all of the components.

Lineup

ParticleUSP L No.Particle size (µm)BondingCharacteristic
C18 L1 3, 5 C18 First Choice
RP-AQUA L62 3, 5 C28 100% aqueous mobile phase is available
C8 L7 3, 5 C8 For high-speed analysis of low-polar compounds
PhE L11 3, 5 Phenylethyl Analysis of separation of aromatic compounds
PFP L43 5 Pentafluorophenyl Analysis for structural isomers and halide