Protocols & FAQ
Protocols
Chemical pulldown
| E001 | Screening by using ligand immobilized beads | 101KB |
|---|---|---|
| E003 | Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads | 90KB |
| E004 | Immobilization of ligands (carboxylic compounds) on OH beads | 67KB |
| E005 | Immobilization of ligands (carboxylic compounds) on NH2 beads | 95KB |
| E008 | Immobilization of ligands (compounds with NH2 groups) on COOH beads | 133KB |
| E012 | Competitive inhibition | 103KB |
| E013 | Drug elution | 101KB |
| E014 | Immobilization of ligands (compounds with NH2 groups) on NHS beads | 88KB |
| E108 | Immobilization of Ligand on Streptavidin beads | 89KB |
| E109 | Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction | 74KB |
| E201 | Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) | 2,345KB |
The protocols above are for immobilization of 2.5 mg of a ligand. For smaller scale, click the link here to get protocols.
Immunoprecipitation, Protein-protein interaction
| E011 | Immunoprecipitation | 100KB |
|---|---|---|
| E101 | Immobilization of proteins on COOH beads | 89KB |
| E102 | Immobilization of His-Tag proteins on Ts beads | 96KB |
| E105 | Immobilization of Antibodies or Proteins on NHS beads | 80KB |
| E106 | Immobilization of Antibodies or Proteins on Epoxy Beads | 78KB |
| E107 | Direct Quantification of Immobilized Proteins (Antibodies) | 102KB |
| E108 | Immobilization of Ligand on Streptavidin beads | 93KB |
| E110 | Immobilization of antibodies on Protein A beads and Protein G beads | 134KB |
Purification of DNA and RNA binding substances
| E001 | Screening by using ligand immobilized beads | 101KB |
|---|---|---|
| E301 | Immobilization of double strand DNA on Plain beads | 137KB |
FAQ
- Are the sizes of FG beads® all equal?
The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.
- What is the linker length of FG beads®?
It is about 1 nm.
- What is the number of particles per 1 mg of FG beads®?
It is about 1.8×1011 particles.
- What types of the FＧ beads are there?
At present, 13 types of FG beads® are available, with different surface modificationssuch as functional groups or bound proteins. Additions to the lineup are currently being considered.
In addition to FG beads®, there are also fluorescent beads. - What is the approximate quantity of the functional groups on FG beads®?
The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.
- Are the FG beads® resistant to heat?
There are no problems up to approximately 70°C. However the type of beads having high functional reactivity of the linker end may come under an influence by the heat.
- Are the FG beads® resistant to pH?
There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.
- Is there the resistance to organic solvents of FG beads® in addition to DMF?
Ligands can be immobilized on the surfaces of FG beads® in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.
- Can the FG beads® be reused?
It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads® and makes it impossible for them to be reused.
- How should one select a bead type?
Select the FG beads® with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads