Chemical pulldown
E001 | Screening by using ligand immobilized beads | 101KB |
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E003 | Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads | 90KB |
E004 | Immobilization of ligands (carboxylic compounds) on OH beads | 67KB |
E005 | Immobilization of ligands (carboxylic compounds) on NH2 beads | 95KB |
E008 | Immobilization of ligands (compounds with NH2 groups) on COOH beads | 133KB |
E012 | Competitive inhibition | 103KB |
E013 | Drug elution | 101KB |
E014 | Immobilization of ligands (compounds with NH2 groups) on NHS beads | 88KB |
E108 | Immobilization of Ligand on Streptavidin beads | 89KB |
E109 | Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction | 74KB |
E201 | Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) | 2,345KB |
The protocols above are for immobilization of 2.5 mg of a ligand. For smaller scale, click the link here to get protocols.
Immunoprecipitation, Protein-protein interaction
E011 | Immunoprecipitation | 100KB |
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E101 | Immobilization of proteins on COOH beads | 89KB |
E102 | Immobilization of His-Tag proteins on Ts beads | 96KB |
E105 | Immobilization of Antibodies or Proteins on NHS beads | 80KB |
E106 | Immobilization of Antibodies or Proteins on Epoxy Beads | 78KB |
E107 | Direct Quantification of Immobilized Proteins (Antibodies) | 102KB |
E108 | Immobilization of Ligand on Streptavidin beads | 93KB |
E110 | Immobilization of antibodies on Protein A beads and Protein G beads | 134KB |
Purification of DNA and RNA binding substances
E001 | Screening by using ligand immobilized beads | 101KB |
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E301 | Immobilization of double strand DNA on Plain beads | 137KB |
The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.
It is about 1 nm.
It is about 1.8×1011 particles.
At present, 13 types of FG beads® are available, with different surface modificationssuch as functional groups or bound proteins. Additions to the lineup are currently being considered.
In addition to FG beads®, there are also fluorescent beads.
The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.
There are no problems up to approximately 70°C. However the type of beads having high functional reactivity of the linker end may come under an influence by the heat.
There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.
Ligands can be immobilized on the surfaces of FG beads® in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.
It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads® and makes it impossible for them to be reused.
Select the FG beads® with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads