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Protocols & FAQ

Protocols

Chemical pulldown

E001 Screening by using ligand immobilized beads 101KB
E003 Immobilization of ligands (compounds with phenol groups or NH2 groups) on epoxy beads 90KB
E004 Immobilization of ligands (carboxylic compounds) on OH beads 67KB
E005 Immobilization of ligands (carboxylic compounds) on NH2 beads 95KB
E008 Immobilization of ligands (compounds with NH2 groups) on COOH beads 133KB
E012 Competitive inhibition 103KB
E013 Drug elution 101KB
E014 Immobilization of ligands (compounds with NH2 groups) on NHS beads 88KB
E108 Immobilization of Ligand on Streptavidin beads 89KB
E109 Immobilization of ligands (alkyne structure compounds) on azide beads using click chemistry reaction 74KB
E201 Quantifying the amount of ligand immobilization by HPLC (High Performance Liquid Chromatography) 2,345KB

The protocols above are for immobilization of 2.5 mg of a ligand. For smaller scale, click the link here to get protocols.

 

Immunoprecipitation, Protein-protein interaction

E011 Immunoprecipitation 100KB
E101 Immobilization of proteins on COOH beads 89KB
E102 Immobilization of His-Tag proteins on Ts beads 96KB
E105 Immobilization of Antibodies or Proteins on NHS beads 80KB
E106 Immobilization of Antibodies or Proteins on Epoxy Beads 78KB
E107 Direct Quantification of Immobilized Proteins (Antibodies) 102KB
E108 Immobilization of Ligand on Streptavidin beads 93KB
E110 Immobilization of antibodies on Protein A beads and Protein G beads 134KB

 

Purification of DNA and RNA binding substances

E001 Screening by using ligand immobilized beads 101KB
E301 Immobilization of double strand DNA on Plain beads 137KB

FAQ

  1. Are the sizes of FG beads® all equal?

    The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.

  2. What is the linker length of FG beads®?

    It is about 1 nm.

  3. What is the number of particles per 1 mg of FG beads®?

    It is about 1.8×1011 particles.

  4. What types of the FG beads are there?

    At present, 13 types of FG beads® are available, with different surface modificationssuch as functional groups or bound proteins. Additions to the lineup are currently being considered.
    In addition to FG beads®, there are also fluorescent beads.

  5. What is the approximate quantity of the functional groups on FG beads®?

    The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.

  6. Are the FG beads® resistant to heat?

    There are no problems up to approximately 70°C. However the type of beads having high functional reactivity of the linker end may come under an influence by the heat.

  7. Are the FG beads® resistant to pH?

    There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.

  8. Is there the resistance to organic solvents of FG beads® in addition to DMF?

    Ligands can be immobilized on the surfaces of FG beads® in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.

  9. Can the FG beads® be reused?

    It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads® and makes it impossible for them to be reused.

  10. How should one select a bead type?

    Select the FG beads® with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads