Nacalai USA - Innovations for Life Sciences

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Protocols & FAQ


  1. Are the sizes of FG beads® all equal?

    The sizes are 0.2 μm. The CV value indicating variation is approximately 30%.

  2. What is the linker length of FG beads®?

    It is about 1 nm.

  3. What is the number of particles per 1 mg of FG beads®?

    It is about 1.8×1011 particles.

  4. What types of the FG beads are there?

    At present, 13 types of FG beads® are available, with different surface modificationssuch as functional groups or bound proteins. Additions to the lineup are currently being considered.
    In addition to FG beads®, there are also fluorescent beads.

  5. What is the approximate quantity of the functional groups on FG beads®?

    The amount of epoxy on Plain beads is 1 μmol per 1 mg of beads. The amount of functional groups on COOH beads and NHS beads is 200 – 300 nmol per 1 mg of beads.

  6. Are the FG beads® resistant to heat?

    There are no problems up to approximately 70°C. However the type of beads having high functional reactivity of the linker end may come under an influence by the heat.

  7. Are the FG beads® resistant to pH?

    There are no problems within the range of pH2 - pH11. However the protein immobilized on beads may denature and the performance may decrease.

  8. Is there the resistance to organic solvents of FG beads® in addition to DMF?

    Ligands can be immobilized on the surfaces of FG beads® in various organic solvents, such as methanol, DMF, DMSO, THF, ethyl acetate, pyridine, dioxane, toluene, dichloromethane, chloroform, etc.

  9. Can the FG beads® be reused?

    It is believed that the beads can be reused when salt elution or drug elution was performed. However boil elution causes degeneration of the FG beads® and makes it impossible for them to be reused.

  10. How should one select a bead type?

    Select the FG beads® with the optimal function group according to the substance or protein that you will bind, or select streptavidin beads