Recombinant SARS-CoV-2 Mpro

Mpro of Severe acute respiratory Coronavirus 2 is essential for viral replication by processing the polyproteins 1ab at 11 cleavage sites. Inhibiting the activity of this enzyme would block viral replication, therefore, it becomes a promising target for the development of anti-coronaviral therapeutic agents.

Synonym(s):	2019-nCoV Mpro, 3CL Protease, 3CL Pro, Main protease, COVID-19
Molecular Weight :	33.8 kDa
Concentration :	0.6 mg/ml
Tag :	No Tag
Source :	Escherichia Coli.
GeneBank Accession # :	P0DTC1.1, Ser3264-Gln3569
Purity :	Greater than 95%, as determined by SDS-PAGE
Formulation :	20 mM Tris-HCl, pH7.8, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 20% Glycerol, 0.02% Na-Azide
Application :	Inhibitor Screening, activity assay
Asssay :	Assay Condition:  The enzymatic reactions are performed at 10µM substrate, indicated amount of Mpro enzyme in 1X assay buffer at room temperature for 2 hours. The fluorescence intensity is measured at exc/em 360 nm/460 nm.
Storage :	Stable for 12 months at -80°C, Avoid freeze/thaw cycles
Reference :	Zhang, L., et al., Science 24 Apr 2020; 368 (6489): 409-412. / Dai, W., et al., Science Jun 19 2020; 368(6497):1331-1335.