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Recombinant Proteins for Coronavirus Research

Recombinant Proteins

Recombinant SARS-CoV-2 Mpro

Mpro of Severe acute respiratory Coronavirus 2 is essential for viral replication by processing the polyproteins 1ab at 11 cleavage sites. Inhibiting the activity of this enzyme would block viral replication, therefore, it becomes a promising target for the development of anti-coronaviral therapeutic agents.

Synonym(s):  2019-nCoV Mpro, 3CL Protease, 3CL Pro, Main protease, COVID-19
Molecular Weight :  33.8 kDa
Concentration :  0.6 mg/ml
Tag :  No Tag
Source : Escherichia Coli.
GeneBank Accession # : P0DTC1.1, Ser3264-Gln3569
Purity :
Greater than 95%, as determined by SDS-PAGE 
Formulation :  20 mM Tris-HCl, pH7.8, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 20% Glycerol, 0.02% Na-Azide
Application :  Inhibitor Screening, activity assay
Asssay : 

Assay Condition:  The enzymatic reactions are performed at 10µM substrate, indicated amount of Mpro enzyme in 1X assay buffer at room temperature for 2 hours. The fluorescence intensity is measured at exc/em 360 nm/460 nm.
Storage :  Stable for 12 months at -80°C, Avoid freeze/thaw cycles
Reference : 
Zhang, L., et al., Science 24 Apr 2020; 368 (6489): 409-412. / Dai, W., et al., Science Jun 19 2020; 368(6497):1331-1335.

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Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Recombinant SARS-CoV-2 Mpro, 3CL Protease, CF NU01022-1 -80 °C 50 µg 299.00 Buy
Recombinant SARS-CoV-2 Mpro, 3CL Protease, CF NU01022-2 -80 °C 500 µg 2,799.00 Buy
Recombinant SARS-CoV-2 Helicase (NSP13) NU01064-1 -80 °C 10 µg 199.00 Buy
Recombinant SARS-CoV-2 Helicase (NSP13) NU01064-2 -80 °C 50 µg 245.00 Buy
Recombinant SARS-CoV-2 Helicase (NSP13) NU01064-3 -80 °C 100 µg 310.00 Buy
Recombinant SARS-CoV-2 Nucleocapsid Phosphoprotein (N) NU01062-1 -80 °C 100 µg 319.00 Buy
Recombinabt SARS-CoV-2 Papain-like Protease (PLpro, NSP3), CF NU01063-1 -80 °C 50 µg 249.00 Buy
Recombinabt SARS-CoV-2 Papain-like Protease (PLpro, NSP3), CF NU01063-2 -80 °C 100 µg 319.00 Buy
Recombinabt SARS-CoV-2 Papain-like Protease (PLpro, NSP3), CF NU01063-3 -80 °C 1 mg 2,699.00 Buy
Recombinant SARS-CoV-2 RNA-dependent RNA polymerase (NSP12) NU01065-1 -80 °C 100 µg 319.00 Buy
Recombinant SARS-CoV-2 RNA-dependent RNA polymerase (NSP7, NSP8, NSP12 Complex) NU01066-1 -80 °C 20 µg 389.00 Buy
Recombinant SARS-CoV-2 NSP7 NU01067-1 -80 °C 100 µg 319.00 Buy
Recombinant SARS-CoV-2 NSP7 NU01067-2 -80 °C 1 mg 2,599.00 Buy
Recombinant SARS-CoV-2 NSP8 NU01068-1 -80 °C 100 µg 319.00 Buy
Recombinant SARS-CoV-2 NSP8 NU01068-2 -80 °C 1 mg 2,599.00 Buy