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COSMO(+)Capillary

COSMO(+)Capillary is cationic polymer coated capillary column, for electroosmotic flow (EOF) is from the chthode (-) to the direction of the anode (+) because of the cationic polymer coating inside of the capillary column.

  • Opposit EOF direction to non-coated capillary
  • High reproducibility
  • Applicable wide pH range (pH2-10)

 

Comparison of COSMO(+)Capillary and Non-coated Capillary

Electropherograms for E.coli Metabolic Extraction

The figure shows the analysis data of various kinds of metabolites. COSMO(+)Capillary can analyze them within 20 minutes by electroosmotic flow (EOF) reversal and a sheath liquid consisting of 50 mM ammonium acetate, pH8.5.

Peak Identification:
1: Glycolate
2: Lactate
3: Fumarate
4: 2-Oxoisopentanoate
5: Succinate
6: Malate
7: PEP
8: DHAP
9: Glycerol3P
10: 3PG
11: MES(is)
12: Gluconate
13: Ribulose5P
14: G1P
15: G6P+F6P
16: 2,3DPG
17: Sedoheptulose7P
18, F1,6DP
Experimental Conditions:
Sample:
Capillary:
Electrolyte:
Applied Potential:
Injection:
Temperature:
Sheath liquid:
E.coli W3110, 109 Cells
COSMO(+) 50 µm I.D. x 110 cm
50 mM Ammonium Acetate,  pH8.5
-30 kV
30 sec at 50 mbar
20°C
10 µl/min 5 mM Ammonium Acetate in 50% (v/v) Methanol - Water

Data courtesy of Prof. Tomoyoshi Soga, Institute for Advanced Biosciences (IAB), Keio University

Application Data: Reproducibility of Reference Standards

The figure shows high reproducibility when COSMO(+)Capillary is used repetitively (26 reference standards).

Experimental Conditions:
Sample Concentration
Capillary
Electrolyte
Applied Potential
Injection
Temperature
Sheath liquid
50 µmol/l each
COSMO(+) 50 µm I.D. x 110 cm
50 mM Ammonium Acetate, pH8.5
-30 kV
30 sec at 50 mbar
20°C
10 µl/min 5 mM Ammonium Acetatein 50% (v/v) Methanol - Water

Data courtesy of Prof. Tomoyoshi Soga, Institute for Advanced Biosciences (IAB), Keio University

Application Data: Acid Analysis of Salivary

The COSMO(+)Capillary enables separation of Succinic Acid which fused silica can not separate. Furthermore it has shorter analysis time for other acids than fused silica.

(a) Lactic Acid (m/z=89)
(b) Succinic Acid (m/z=117)
(c) Malic Acid (m/z=133)
(d) Uric Acid (m/z=167)
(e) Citrate (m/z=191)
Experimental Conditions:
Capillary
Electrolyte
Applied Potential
Injection
Temperature
Sheath liquid
COSMO(+) 50 µm I.D. x 110 cm
50 mM ammonium acetate, pH8.5
-30 kV
-10 kV × 30 sec
25°C
10 mM Ammonium Carbonate: 2-Propanol =1:1

Data courtesy of Yoshihide Tanaka, National Institute of Advanced Industrial Science and Technology

References

  1. Zhang, Wei, and Rawi Ramautar. "CE‐MS for metabolomics: Developments and applications in the period 2018–2020." Electrophoresis 42, no. 4 (2021): 381-401.
  2. Ishibashi, Yoshiki, Sei Harada, Ayano Takeuchi, Miho Iida, Ayako Kurihara, Suzuka Kato, Kazuyo Kuwabara et al. "Reliability of urinary charged metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry." Scientific reports 11, no. 1 (2021): 1-9.
  3. Saito, Rintaro, Akiyoshi Hirayama, Arisa Akiba, Yushi Kamei, Yuyu Kato, Satsuki Ikeda, Brian Kwan et al. "Urinary Metabolome Analyses of Patients with Acute Kidney Injury Using Capillary Electrophoresis-Mass Spectrometry." Metabolites 11, no. 10 (2021): 671.
  4. Miyamoto, Kana, Akiyoshi Hirayama, Yuiko Sato, Satsuki Ikeda, Midori Maruyama, Tomoyoshi Soga, Masaru Tomita et al. "A Metabolomic Profile Predictive of New Osteoporosis or Sarcopenia Development." Metabolites 11, no. 5 (2021): 278.
  5. Akiyama, Yasutoshi, Koichi Kikuchi, Takafumi Toyohara, Eikan Mishima, Chitose Suzuki, Takehiro Suzuki, Masaaki Nakayama, Yoshihisa Tomioka, Tomoyoshi Soga, and Takaaki Abe. "CE-MS-Based Identification of Uremic Solutes Specific to Hemodialysis Patients." Toxins 13, no. 5 (2021): 324.
  6. Guo, Jing, Kiyotoshi Satoh, Sho Tabata, Masaru Mori, Masaru Tomita, and Tomoyoshi Soga. "Reprogramming of glutamine metabolism via glutamine synthetase silencing induces cisplatin resistance in A2780 ovarian cancer cells." BMC cancer 21, no. 1 (2021): 1-11.
  7. Kozawa, Kei, Miho Sekai, Kenji Ohba, Shoko Ito, Hiroaki Sako, Takeshi Maruyama, Mai Kakeno et al. "The CD44/COL17A1 pathway promotes the formation of multilayered, transformed epithelia." Current Biology (2021).

Ordering Information

Product Cat.No. PKG Size Price(US$)  
COSMO(+) Capillary (50 µmI.D. x 120 cm) 07584-44 2 pkg 690.00 Buy