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Recombinant Human eIF4E

Eukaryotic Translation Initiation Factor 4E

Human Eukaryotic translation initiation factor 4E (eIF4E) is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. eIF4E is part of the eIF4F pre-initiation complex, which is made up of eIF4E, and eIF4G. Almost all cellular proteins require eIF4E in order to be translated into protein. eIF4E binds the first nucleotide on the 5' end of an mRNA molecule (known as the cap): a 7- methylguanosine (m7G). It sandwiches m7G between 2 tryptophan residues, and other amino acids are involved in the binding. Recombinant human eIF4E is expressed in E. Coli as a single, nonglycosylated polypeptide chain containing 237 amino acids including eIF4E (1-217 a.a.) with a N-terminal His-tag. It is well purified by affinity, ion exchange and gel filtration chromatographic techniques.

 

Synonym(s):  eIF4E1, eIF4EL1, CBP
Molecular Weight :  27.3 kDa
Tag :  N-terminal His Tag
Source : Escherichia Coli.
Purity :
Greater than 98%, as determined by SDS-PAGE 
Formulation :  0.5 mg/ml eIF4E with 2mM DTT, 10% glycerol, 0.02% NaN3, 150mM NaCl , 50mM Tris-HCl pH7.5.
Application :  Binding assay, small molecule inhibitor screening, crystallization/co-crystallization, substrate of MNK1,2 or other kinase
Storage :  Stable for 12 months at -80 °C, stable for 2-4 weeks at 4°C, avoid multiple freeze-thaw cycles.
Reference : 
Kiyotaka Mochizuki, et al, RNA. 2005 Jan;11(1):77-89.
Raught B1, Gingras AC., Int J Biochem Cell Biol. 1999 Jan;31(1):43-57.
Salehi Z., Mashayekhi F. and Shahosseini F. Cell Biol. Int. 31 (11), 1400-1404 (2007).

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Recombinant Human eIF4E, His-tagged NU03061-1 -80oC 2 µg 50.00 Buy
Recombinant Human eIF4E, His-tagged NU03061-2 -80oC 10 µg 130.00 Buy
Recombinant Human eIF4E, His-tagged NU03061-3 -80oC 100 µg 399.00 Buy
Recombinant Human eIF4E, His-tagged NU03061-4 -80oC 1 mg N/A Inquire
Recombinant Human eIF4E complexes with eIF4G, His-tagged NU03058-1 -80oC 2 µg 50.00 Buy
Recombinant Human eIF4E complexes with eIF4G, His-tagged NU03058-2 -80oC 10 µg 130.00 Buy
Recombinant Human eIF4E complexes with eIF4G, His-tagged NU03058-3 -80oC 100 µg 399.00 Buy
Recombinant Human eIF4E complexes with eIF4G, His-tagged NU03058-4 -80oC 1 mg N/A Inquire
Recombinant Human eIF4E complexes with m7GTP NU03059-1 -80oC 2 µg 50.00 Buy
Recombinant Human eIF4E complexes with m7GTP NU03059-2 -80oC 10 µg 130.00 Buy
Recombinant Human eIF4E complexes with m7GTP NU03059-3 -80oC 100 µg 399.00 Buy
Recombinant Human eIF4E complexes with m7GTP NU03059-4 -80oC 1 mg N/A Inquire