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Recombinant Human MNK2

MAP kinase-interacting serine/threonine-protein kinase 2 is an enzyme encoded by MKNK2 gene. It contains a conserved C-terminal ERK-interacting domain, a catalytic domain with homology to calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain. MNK2 binds tightly to growth factor-regulated MAP kinases, ERK1 and ERK2. ERK and p38 phosphorylate MNK2, which stimulates its in vitro kinase activity toward a substrate, eukaryotic initiation factor-4E (EIF4E). MNK2 (amino acid residues 72–385), which includes the kinase domain, is expressed in E. coli as a single, non-glycosylated polypeptide chain. It is well purified by affinity, ion exchange and gel filtration chromatographic techniques.
 

Source : E. coli
Purification :
affinity, ion exchange and gel filtration chromatographic techniques
Purity :

>97% on SDS PAGE and HPLC analyses.

Formulation : 
0.5 mg/ml MNK2 D228G, 2 mM DTT, 20% glycerol, 0.02% NaN3, 0.1 mM EDTA, 150 mM NaCl , 50 mM Tris-HCl pH7.5.
Storage : 
Upon delivery aliquot and store at -80 °C. Avoid multiple freeze-thaw cycles. The protein is stable for 12 months at -80 °C, for 2-4 weeks at 4°C.
Suggested Usage : 
Protein binding assay, small molecule inhibitor screening, substrate of kinase, antigen, ELISA, Western blot, crystallization and co- crystallization studies.

Ordering Information

Product Cat.No. Purity Storage PKG Size Price(US$)  
Recombinant Human MNK2 NU03023-1 >97% on SDS PAGE and HPLC analyses. -80°C 2 ug 84.00 Buy
Recombinant Human MNK2 NU03023-2 >97% on SDS PAGE and HPLC analyses. -80°C 10 ug 137.00 Buy
Recombinant Human MNK2 NU03023-3 >97% on SDS PAGE and HPLC analyses. -80°C 100 ug 734.00 Buy
Recombinant Human MNK2 NU03023-4 >97% on SDS PAGE and HPLC analyses. -80°C 1 mg 5,249.00 Buy
Recombinant Human MNK2, His-tagged NU03024-1 >97% on SDS PAGE and HPLC analyses. -80°C 2 ug 84.00 Buy
Recombinant Human MNK2, His-tagged NU03024-2 >97% on SDS PAGE and HPLC analyses. -80°C 10 ug 137.00 Buy
Recombinant Human MNK2, His-tagged NU03024-3 >97% on SDS PAGE and HPLC analyses. -80°C 100 ug 734.00 Buy
Recombinant Human MNK2, His-tagged NU03024-4 >97% on SDS PAGE and HPLC analyses. -80°C 1 mg 5,249.00 Buy
Recombinant Human MNK2 (D2228G) NU03049-1 >97% on SDS PAGE and HPLC analyses. -80°C 2 ug 84.00 Buy
Recombinant Human MNK2 (D2228G) NU03049-2 >97% on SDS PAGE and HPLC analyses. -80°C 10 ug 137.00 Buy
Recombinant Human MNK2 (D2228G) NU03049-3 >97% on SDS PAGE and HPLC analyses. -80°C 100 ug 734.00 Buy
Recombinant Human MNK2 (D2228G) NU03049-4 >97% on SDS PAGE and HPLC analyses. -80°C 1 mg 5,249.00 Buy
Recombinant Human MNK2 (D2228G), His-tagged NU03050-1 >97% on SDS PAGE and HPLC analyses. -80°C 2 ug 84.00 Buy
Recombinant Human MNK2 (D2228G), His-tagged NU03050-2 >97% on SDS PAGE and HPLC analyses. -80°C 10 ug 137.00 Buy
Recombinant Human MNK2 (D2228G), His-tagged NU03050-3 >97% on SDS PAGE and HPLC analyses. -80°C 100 ug 734.00 Buy
Recombinant Human MNK2 (D2228G), His-tagged NU03050-4 >97% on SDS PAGE and HPLC analyses. -80°C 1 mg 5,249.00 Buy