This kit includes reagents for reverse transcription and for the removal of genomic DNA [DNase I treatment].
In many cases, total RNA prepared using spin-columns or acid guanidium-phenolchloroform (AGPC) extraction methods contains small amount of genomic DNA. Any contaminating genomic DNA will be amplified along with cDNA, especially when primer pairs are designed within the same exon or from pseudogenes. Amplification from genomic DNA can result in qualitative and quantitative inaccuracies. The protocol consists of (i) a genomic DNA degradation step using "gDNA remover" and (ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.