THUNDERBIRD^TM Next Probe One-step qRT-PCR 4×Mix is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) mix that uses the highly efficient reverse transcriptase “ReverTra Ace^TM” and Hot Start TTx DNA Polymerase. The combination of these two enzymes and our optimized buffer system enables the quickly and highly sensitively detection and quantification of small quantities of RNA. This mix does not contain glycerol and contains a lyophilization stabilizer; therefore, there should be no need to add any further excipients to assist lyophilization. After freeze-drying, it can be stored stably at room temperature and transported without the need for ice packs. In addition, real-time PCR reactions can be performed immediately by simply adding a sample containing a template and water. Freeze-drying does not impact the performance of this PCR mix.