Hot Start TTx (DNA) Kit is qPCR reagent based on our original polymerase, TTx DNA Polymerase. TTx DNA Polymerase has higher amplification efficiency than Taq DNA Polymerase, which is a general-purpose enzyme, and enables amplification using fast cycle condition and amplification from a crude sample containing PCR inhibitors.
In addition, TTx DNA Polymerase has a 5 '- 3' exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan ^® assay. This enzyme contains neutralizing antibodies, thus allowing for Hot start PCR.