This is a one-step qRT-PCR reagent using our unique enzyme TTx DNA Polymerase. TTx DNA Polymerase has reverse transcriptase activity, and this activity is strongly promoted in the presence of Mn²⁺. This activity can be used to perform reverse-transcription reactions and PCR with the same enzyme. Because TTx DNA Polymerase has reverse transcriptase activity, it can amplify with high efficiency from both DNA and RNA. TTx DNA Polymerase also has 5'→3' exonuclease activity, so it can be used for real-time PCR, for example using TaqMan^® probes. This reagent contains a mixture of neutralizing antibodies, allowing for highly specific Hot Start PCR.