GenNext™ RamDA-seq™ Single Cell Kit is a kit using RT-RamDA™ (Reverse Transcription with Random Displacement Amplification) method for preparing cDNA for NGS analysis from single-cell and Ultra-low amount RNA.
RT-RamDA™ is a novel cDNA amplification method utilizing the strand displacement activity of reverse-tr​anscriptas​e. GenNext RamDA-seq™ Single Cell Kit can detect not only poly(A) RNA but also non-poly(A) with high sensitively by using random primer or NSR primer^*, allowing the detection of many genes compared with the conventional method.
Unlike conventional method using Oligo-dT primer, cDNA for full-length total RNA-seq can be synthesized by RT-RamDA™ method.
In addition, because cDNA is amplified at the same time as it is synthesized, RamDA-seq™ method does not require amplification adaptors and PCR amplification step, and it reduces the biases caused by PCR amplification.