The QuantAccuracy™, RT-RamDA™ cDNA Synthesis Kit (Code No. RMQ-101) is an efficient and convenient kit to synthesize cDNA from single cells or trace amounts of RNA for real-time PCR analysis.
By using this kit, cDNA covering full-length total RNA can be synthesized, and gene expression analysis can be performed with high sensitivity.
This kit uses reverse transcription with random displacement amplification (RT-RamDA™) (see method in reference (1)). RT-RamDA™ is a novel cDNA amplification method that utilizes the strand displacement activity of reverse transcriptase, which can detect not only poly(A) RNA but also non-poly(A) RNA with high sensitivity. For this reason, the RT-RamDA™ method is characterized by its ability to detect more genes than conventional technology.
*RT-RamDA™ and RamDA-seq™are trademarks of RIKEN, Institute of Physical and Chemical Research, Japan
Reference (1) Tetsutaro Hayashi*, Haruka Ozaki*, Yohei Sasagawa, Mana Umeda, Hiroki Danno and Itoshi Nikaido. Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. Nature Communications. 2018.