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COSMOCORE 2.6PBr

Features

  • Separate hydrophilic compounds in reversed-phase conditions
  • Retain hydrophilic compounds longer than C18
  • Greater sample loading capacity than HILIC
  • High performance similar to sub-2 µm particles with lower back pressure
Suitable Samples
  • Hydrophilic compounds
  • Nucleic acids and derivatives
  • Surfactants
  • Glycosides
  • Peptides

» Information on COSMOSIL 5PBr (fully porous particles)

Packing MaterialCOSMOCORE 2.6PBr
Silica gel Core-shell type silica gel
Average particle size 2.6 µm
Average pore size approx. 90Å
Specific surface area approx. 150m2/g
Stationary phase Pentabromobenzyl Group
Endcapping treatment Yes
Usable pH range 2 - 7.5
Maximum puressure 60MPa

>> Click here to COSMOCORE Development Kit 

Separation of Hydrophilic Compounds (low retention on C18)

COSMOSIL PBr retains hydrophilic compounds stronger than C18 columns under the same reversed-phase conditions.

Nucleic Acid Metabolites

Column: COSMOCORE **
Column size: 2.1mmI.D.-100mm
Mobile phase: Methanol/ 100mmol/l Phosphate buffer(pH2.5) = 10/90
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV240nm
Sample: 1; Allantoin (2.0mg/ml)
2; Guanine (0.05mg/ml)
3; Hypoxanthine (0.05mg/ml)
4; Uric Acid (0.1mg/ml)
5; Xanthine (0.2mg/ml)
6; Adenosine (0.2mg/ml)
7; Inosine (0.1mg/ml)
Inj. Vol: 1.0µl

Malonyl CoA, CoA, Acetyl CoA

Column: COSMOCORE **
Column size: 2.1mmI.D.-100mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 5/95
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV260nm
Sample: 1; Malonyl CoA (0.5mg/ml)
2; CoA (0.5mg/ml)
3; Acetyl CoA (0.5mg/ml)
Inj. Vol: 1.0µl

Water-Soluble Vitamins

Column: COSMOCORE 2.6PBr
Column size: 2.1mmI.D.-150mm
Mobile phase: A; 20mmol/l Phosphate buffer(pH2.5)
B; Methanol/ 20mmol/l P hosphate buffer(pH2.5) = 60/40
B conc. 0% (0-1min), 0→ˆ80% (1→ˆ5min), 80→ˆ100% (5ˆ→9min)
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV220nm
Sample: 1; Vitamin B1 [Thiamine] (0.25mg/ml)
2; Vitamin C [Ascorbic Acid] (1.0mg/ml)
3; Vitamin B3 [Nicotinic Acid] ( 0.04mg/ml)
4; Vitamin B3 [Nicotinamide] ( 0.06mg/ml)
5; Vitamin B6 [Pyridoxine] (0.25mg/ml)
6; Vitamin B5 [Pantothenic Acid] (2.0mg/ml)
7; Vitamin B9 [Folic Acid] (0.2mg/ml)
8; Vitamin B7 [Biotin] (3.0mg/ml)
9; Vitamin B12 [Cyanocobalamin] (0.25mg/ml)
10; Vitamin B2 [Riboflavin] (0.2mg/ml)
Inj. Vol: 1.25µl

Separation of Hydrophilic Compounds (compounds with similar hydrophobicity)

COSMOCORE 2.6PBr can separate compounds with similar hydrophobicity, utilizing several kinds of molecular interactions, including dispersion force generated by the bromine atoms.

ATP, ADP, AMP

Column: COSMOCORE **
Column size: 2.1mmI.D.-100mm
Mobile phase: 20mmol/l Phosphate buffer(pH7.0)
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV260nm
Sample: 1; ATP (0.5mg/ml)
2; ADP (0.8mg/ml)
3; AMP (0.8mg/ml)
Inj. Vol: 0.5µl

Vitamin B3 (Nicotinic Acid, Nicotinamide)

Column: COSMOCORE 2.6PBr
Column size: 2.1mmI.D.-150mm
Mobile phase: 10mmol/l Phosphate buffer(pH7.0)
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV260nm
Sample: 1; Nicotinic Acid (0.4mg/ml)
2; Nicotinamide (0.6mg/ml)
Inj. Vol: 0.5µl

Thiamine Pyrophosphate, Thiamine

Column: COSMOCORE 2.6PBr
Column size: 2.1mmI.D.-150mm
Mobile phase: 100mmol/l Phosphate buffer(pH2.5)
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV245nm
Sample: 1; Thiamine Pyrophosphate (0.4mg/ml)
2; Thiamine (0.4mg/ml)
Inj. Vol: 0.5µl

UDP Glycosides

Column: COSMOCORE 2.6PBr
Column size: 2.1mmI.D.-150mm
Mobile phase: 100mmol/l Phosphate buffer(pH7.0)
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV260nm
Sample: 1; UDP (0.8mg/ml)
2; UDP-Galactose (0.8mg/ml)
3; UDP-Glucose (0.8mg/ml)
Inj. Vol: 0.5µl

Arbutin and Hydroquinone

Column: COSMOCORE **
Column size: 2.1mmI.D.-100mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate buffer(pH2.5)
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV290nm
Sample: 1; Arbutin (1.33mg/ml)
2; Hydroquinone (0.67mg/ml)
Inj. Vol: 0.75µl

Separation Mechanism

  • Dispersion force (instantaneous dipole–induced dipole force)
    London dispersion force is a weak intermolecular force that results from dipoles temporarily induced by random unsymmetrical electron positions in two adjacent atoms, also known as instantaneous dipole–induced dipole force. It is present in all molecules regardless of polarity. Compounds with high polarizability have stronger dispersion force.
  • Compounds with stronger dispersion force
    • Larger and heavier molecules
    • Molecules with larger and heavier atoms (e.g. from weakest to strongest in halogens, F2, Cl2, Br2, and I2)
    • Molecules with delocalized electrons and resonance (e.g. aromatic compounds)

COSMOSIL PBr column is packed with pentabromobenzyl-bonded silica that enables separation by dispersion force interaction.

Difference between Methanol and Acetonitrile Mobile Phases

Comparison of Separation Ability
Acetonitrile is frequently used in HPLC to reduce backpressure. However, the π electrons in acetonitrile interfere with the dispersion force interaction between the sample and the stationary phase. Therefore, we recommend using methanol as the organic solvent.

Column: COSMOCORE 2.6PBr
Column size: 2.1mmI.D.-100mm
Mobile phase: **
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV210nm
Sample: 1; Stevioside (5.0mg/ml)
2; RebaudiosideA
Inj. Vol: 1.0µl

Comparison of Pressure
In general, methanol/water mobile phases exhibit significantly higher pressure than acetonitrile/water. However, the pressure never exceeded 30 MPa in our test of a 2.1 mm x 100 mm column. Since COSMOSORE 2.6PBr can withstand 60 MPa, methanol-based mobile phases are usable even with UHPLC.

Column: COSMOCORE 2.6PBr (2.1mmI.D.-100mm)
Mobile phase: See graph
Temperature: 40°C
Flow rate: 0.4 ml/min

Comparison to HILIC

Both columns are suitable for analysis of hydrophilic compounds, but they have different properties.

  PBr HILIC
Separation Mode  Reversed Phase Separation Mode
Features
  • Simple mobile phase conditions compared to HILIC.
  • Strong eluent: organic (methanol) Weak eluent: water
  • Low peak distortion with water-based samples; usable with large injection volumes of dilute samples.
  • Some hydrophilic compounds are not retained well.
  • Retains hydrophilic compounds that would not be retained by C18 columns.
  • Strong eluent: water Weak eluent: organic (acetonitrile)
  • Peak distortion occurs with large volumes of samples dissolved in water.

COSMOSIL Application

Ordering Information

Column
Product Cat.No. Quantity Price(US$)  
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 30mm 13692-21 1 pkg 570.00 Buy
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 50mm 13693-11 1 pkg 580.00 Buy
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 75mm 13694-01 1 pkg 630.00 Buy
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 100mm 13695-91 1 pkg 690.00 Buy
COSMOCORE 2.6PBr Packed Column, 2.1mmI.D. x 150mm 13697-71 1 pkg 740.00 Buy
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 30mm 13698-61 1 pkg 570.00 Buy
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 50mm 13699-51 1 pkg 580.00 Buy
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 75mm 13700-01 1 pkg 630.00 Buy
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 100mm 13701-91 1 pkg 690.00 Buy
COSMOCORE 2.6PBr Packed Column, 3.0mmI.D. x 150mm 13703-71 1 pkg 740.00 Buy
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 30mm 13705-51 1 pkg 570.00 Buy
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 50mm 13712-51 1 pkg 580.00 Buy
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 75mm 13714-31 1 pkg 630.00 Buy
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 100mm 13715-21 1 pkg 690.00 Buy
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 150mm 13719-81 1 pkg 740.00 Buy
COSMOCORE 2.6PBr Packed Column, 4.6mmI.D. x 250mm 13734-71 1 pkg 840.00 Buy
Prefilter
Product Cat.No. Quantity Price(US$)  
U-Fil UHPLC-compatible prefilter 12571-31 1 pkg 160.00 Buy