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COSMOCORE 2.6Cholester

Core-Shell Particle

Features

  • Cholesterol-bonded reversed phase core-shell column
  • Use under the same condition as C18 columns
  • Better selectivity for cis-trans isomers, polyphenols, and natural products

Packing Material COSMOCORE 2.6Cholester
Silica gel Core-shell type silica gel
Average particle size 2.6 µm
Average pore size approx. 90Å
Specific surface area approx. 150 m2/g
Stationary phase Cholesteryl Group
Endcapping treatment Yes
Usable pH range  2 - 7.5
Maximum pressure 60 MPa

>> Click here to COSMOCORE Development Kit 

Comparison with C18

COSMOCORE 2.6Cholester offers improved separation for cis-trans isomers than C18 under typical reversed-phase mobile phase.

Column size: 2.1 mmI.D. x 100 mm
Mobile phase: Methanol
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV 280 nm
Sample: 1. Vitamin K2 (0.50 mg/ml)
2. Trans-Vitamin K1 (0.50 mg/ml)
3. Cis-Vitamin K1
Inj. Vol.: 0.5 µl

Separation Properties

COSMOCRE 2.6Cholester has the same hydrophobicity as C18. It is not necessary to change the analytical conditions when replacing C18 Columns with COSMOCORE 2.6Cholester.

Column size: 2.1 mmI.D. x 100 mm
Mobile phase: Acetonitrile : H2O = 50 : 50
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Uracil
2. Methyl Benzoate
3. Toluene
4. Naphthalene
Inj. Vol.: 0.25 µl

Molecular Shape Selectivity

COSMOCORE 2.6Cholester has excellent shape slectivity due to its structural rigidity. COSMOCORE 2.6Cholester retains planar Triphenylene longer than Triphenylene stereoscopic o-Terphenyl.

Column size: 2.1 mmI.D. x 100 mm
Mobile phase: Acetonitrile : H2O = 70 : 30
Flow rate: 0.4 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. o-Terphenyl (0.25 mg/ml)
2. Triphenylene (0.50 mg/ml)
Inj. Vol.: 0.5 µl

Scaling Up from Analytical to Preparative Separation

COSMOCORE 2.6Cholestrer has the same functional group as the fully-porous COSMOSIL Cholester 5 µm silica packing material. Therefore, the separation pattern in COSMOCORE 2.6Cholester is maintained when scaling up to preparative separation with 5 µm COSMOSIL Cholester.

Mobile phase: Methanol
Flow rate: (2.1 mmI.D.) 0.4 ml/min
(4.6 mmI.D.) 1.0 ml/min
(10.0 mmI.D.) 5.0 ml/min
Temperature: 40°C
Detection: UV 280 nm
Sample: 1. trans-Vitamin K1
2. cis-Vitamin K1

The number of reference for COSMOSIL Cholesterol Bonded Stationary Phase

The number of COSMOSIL Cholester reference has grown to over 100 since its introduction in 2005.
(Reference Search: Google Scholar, as of July 2014)

Figure 1: The number of reference of COSMOSIL Cholester and 2.5Cholester

» Information on COSMOSIL Cholester (5 µm)
» Information on COSMOSIL 2.5Cholester (2.5 µm)

Application: Separation of natural components (Tomato Components)

Lycopene exists in many cis-trans isomers. COSMOCORE 2.6Cholester separates these compounds better than C18.

Column size: 3.0 mmI.D. x 50 mm
Mobile phase: (2.6C18) Acetonitrile : H2O = 95 : 5
(2.6Cholester) Acetonitrile
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 470 nm
Sample: 1. Lycopene
2. β-Carotene
Inj. Vol.: 0.5 µl

Application: Separation of Catechin

Column size: 2.1 mmI.D. x 50 mm
Mobile phase: A; Acetonitrile : 0.1% H3PO4 = 10 : 90
B; Acetonitrile : 0.1% H3PO4 = 40 : 60
B conc. 0 → 100% 3 min Linear gradient
Flow rate: 0.6 ml/min
Temperature: 30°C
Detection: UV 280 nm
Sample: 1. (-)-Gallocatechin[(-)-GC]
2. Caffeine
3. (-)-Epigallocatechin[(-)-EGC]
4. (-)-Catechin[(-)-C]
5. (-)-Epicatechin[(-)-EC]
6. (-)-Epigallocatechin G allate[(-)-EGCg]
7. (-)-Gallocatechin Gallate[(-)-GCg]
8. (-)-Epicatechin Gallate[(-)-ECg]
9. (-)-Catechin Gallat[(-)-Cg]

Application: Apply to LC/MS

Column size: 3.0 mmI.D. x 50 mm
Mobile phase: Acetonitrile
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: APCI-MS, Positive, SIM
Sample: Tomato extract
1. Lycopene
2. β-Carotene
Inj. Vol.: 0.5 µl

COSMOSIL Application

Ordering Information

Column
Product Cat.No. Quantity Price(US$)  
COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 30mm 12858-91 1 pkg 570.00 Buy
COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 50mm 12859-81 1 pkg 580.00 Buy
COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 75mm 12860-41 1 pkg 630.00 Buy
COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 100mm 12861-31 1 pkg 690.00 Buy
COSMOCORE 2.6Cholester Packed Column, 2.1mmI.D. x 150mm 12862-21 1 pkg 740.00 Buy
COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 30mm 12863-11 1 pkg 570.00 Buy
COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 50mm 12864-01 1 pkg 580.00 Buy
COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 75mm 12866-81 1 pkg 630.00 Buy
COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 100mm 12867-71 1 pkg 690.00 Buy
COSMOCORE 2.6Cholester Packed Column, 3.0mmI.D. x 150mm 12868-61 1 pkg 740.00 Buy
COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 30mm 12869-51 1 pkg 570.00 Buy
COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 50mm 12870-11 1 pkg 580.00 Buy
COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 75mm 12871-01 1 pkg 630.00 Buy
COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 100mm 12872-91 1 pkg 690.00 Buy
COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 150mm 12873-81 1 pkg 740.00 Buy
COSMOCORE 2.6Cholester Packed Column, 4.6mmI.D. x 250mm 12875-61 1 pkg 840.00 Buy
Prefilter
Product Cat.No. Quantity Price(US$)  
U-Fil UHPLC-compatible prefilter 12571-31 1 pkg 160.00 Buy