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An Alternative HILIC Column

(U)HPLC/SFC Application

Hydrophilic interaction chromatography (HILIC) is a popular technique to analyze very polar molecules. However, it is often difficult to develop a robust method.

HILIC Columns: Disadvantages
・ Extremely sensitive to sample solvent used resulting in shifting retention time.
・ Small sample loading capacity – polar molecules do not dissolve well in organic solvents.
・ Peak distortion occurs if sample solvents are different than the mobile phase.

PBr Columns: Advantages
・ Retain very polar compounds in reversed-phase.
・ Use simple mobile phase, e.g. 100% aqueous or organic mobile phase.
・ Higher sample loading capacity than HILIC.
・ Robust
COSMOCORE (Core-shell based)
COSMOSIL (Fully porus silica gel based)

posted 05/13/2016 12:04