Direct PCR of 5 Genes from 23 Different Human Blood Samples

• PCR: 23 different human blood samples (1µL sample volumes treated with EDTA was added to 50 µl of Ampdirect ® Plus mixture)
• Lane M: Molecular size marker

Direct PCR from fresh and frozen blood

• Target: Human beta-globin gene
• PCR: 5 µl (lane 1), 2.5 µl (lane 2), 1.25 µl (lane 3), 0.63 µl (lane 4) and 0 µl (lane N) of human blood / 50 µl of Ampdirect mixture
• Molecular size marker: ΦX174 RF DNA digested with HincII (lane M)

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