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Ampdirect ® Plus

Real Direct PCR Buffer

Ampdirect ® Plus is a novel PCR buffer that effectively neutralizes DNA polymerase inhibitors present in tissues or body fluids from plants or animals, and allows direct PCR amplification from as little as 0.5 µl of samples.

Features

  • Direct PCR amplification from tissues or body fluids (including blood) from plants or animals
  • No DNA extraction required
  • Minimizes the risk of sample cross contamination
  • Requires small volume of blood (0.5 - 1 µl)
  • Compatible with various anticoagulants (including sodium citrate, dipotassium EDTA and sodium heparinate)
  • Compatible with blood stored frozen for long periods
  • Ideal for genotyping and genetic screening experiments

*Ampdirect ® Plus is produced by SHIMADZU BIOTECH in JAPAN.

Principle

Application 1

Direct PCR of 5 Genes from 23 Different Human Blood Samples

  • PCR: 23 different human blood samples (1µL sample volumes treated with EDTA was added to 50 µl of Ampdirect ® Plus mixture)
  • Lane M: Molecular size marker

Application 2

Direct PCR from fresh and frozen blood

  • Target: Human beta-globin gene
  • PCR: 5 µl (lane 1), 2.5 µl (lane 2), 1.25 µl (lane 3), 0.63 µl (lane 4) and 0 µl (lane N) of human blood / 50 µl of Ampdirect mixture
  • Molecular size marker: ΦX174 RF DNA digested with HincII (lane M)

Technical Information

Ampdirect® Plus Procedure (PDF)

Reference

  1. Urabe, Misako, et al. "Description and molecular characteristics of Morishitium polonicum malayense Urabe, Nor Hashim & Uni, n. subsp.(Trematoda: Cyclocoelidae) from the Asian glossy starling, Aplonis panayensis strigata (Passeriformes: Sturnidae) in Peninsular Malaysia." Parasitology international 76 (2020): 102074.
  2. Sugita, Norimasa, et al. "Non-destructive DNA extraction from herbarium specimens: a method particularly suitable for plants with small and fragile leaves." Journal of plant research 133.1 (2020): 133-141.
  3. El Daous, Hala, et al. "Establishment of a novel diagnostic test for Bovine leukemia virus infection using direct filter PCR." Transboundary and Emerging Diseases (2020).
  4. Chiaki, Yuya, et al. "Dwarfing caused by viral pathogens and leaf malformations in ‘Shine Muscat’grapevine." Journal of General Plant Pathology 86.1 (2020): 34-38.
  5. Matozaki, Toshinori, et al. "Hirticrusta gen. nov. segregated from Neofomitella in Polyporaceae (Polyporales)." Mycoscience (2020).
  6. Squarre, David, et al. "Diversity of trypanosomes in wildlife of the Kafue ecosystem, Zambia." International Journal for Parasitology: Parasites and Wildlife (2020).
  7. Hashiguchi, Yoshihisa, et al. "Natural Leishmania (Leishmania) mexicana infection and biting activity of anthropophilic sand fly Lutzomyia ayacuchensis in the Ecuadorian Andes." Acta Tropica 203 (2020): 105321.
  8. Gaithuma, Alex, et al. "Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies." Scientific reports 10.1 (2020): 1-10.
  9. Alex, Gaithuma, et al. "Blood meal sources and bacterial microbiome diversity in wild-caught tsetse flies." Scientific Reports (Nature Publisher Group) 10.1 (2020).
  10. Hosaka, Kazuyoshi, and Rena Sanetomo. "Broadening Genetic Diversity of the Japanese Potato Gene Pool." American Journal of Potato Research (2020): 1-16.
  11. Genotyping of single nucleotide polymorphism (SNPs) influencing drug response by competitive allele-specific short oligonucleotide hybridization (CASSOH) with immunochromatographic strip
    Hiratsuka M, et al.: Drug Matab. Pharmacokin. 2004;19: 303-7
  12. Rapid and simple mutation screening of GM1 gangliosidosis in Shiba dogs by direct amplification of deoxyribonucleic acid from various forms of canine whole-blood specimens
    Yamato O, et al.: J Vet Diagn Invest 2004;16: 469-72
  13. Prenatal origin of TEL-AMLI-positive acute lymphoblastic leukemia in children born in California
    McHale CM, et al GENES, Chromosomes & Cancer 2003;37: 36-43
  14. In utero origin of t(8;21) AML1-ETO translocations in childhood acute myeloid leukemia
    Wiemels JL, et al.: Blood 2002;99: 3801-5
  15. Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia
    Wiemels JL, et al.: Proc. Natl. Acad. Sci. USA 2002;99: 15101-6
  16. Various applications of direct PCR using blood samples
    Nishimura N, et al.: Clin Lab; 2002;48: 377-84
  17. Efficiency of polymerase chain reaction using a novel method of DNA preparation and amplification for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples
    Kojima K, et al.: Proceedings of the Seventh International Colloquium on Paratuberculosis; 2002; 267-9
  18. Major change in the predominant type of "Norwalk-like viruses" in outbreaks of acute nonbacterial gastroenteritis in Osaka city, Japan, between April 1996 and March 1999
    Iritani, et al.: J Clin Microbiol 2000;38: 2649-54
  19. Detection of clonotypic IGH and TCR rearrangements in the neonatal blood spots of infants and children with B-cell precursor acute lymphoblastic leukemia
    Yagi T, et al.: Blood; 2000;96:264-8
  20. Direct PCR from whole blood without DNA isolation
    Nishimura N, et al.: Ann Clin Biochem; 2000;37: 674-80
  21. Association between platelet glycoprotein Iba genotype and ischemic cerebrovascular disease
    Sonoda A, et al.: Stroke 2000;31: 493-7
  22. Prenatal origin of acute lymphoblastic leukaemia in children
    Wiemels JL, et al.: Lancet 1999;354: 1499-503
  23. Direct amplification of Escherichia coli O157 vero toxin genes from human faeces by the polymerase chain reaction
    Okamoto H, et al.: Ann Clin Biochem 1999;36: 642-8

Ordering Information

Product Cat.No. Storage Quantity Price(US$)  
Ampdirect ® Plus (#241-08800-98) 07199-20 -20 °C 500 reactions 350.00 Buy
Ampdirect ® Plus / BIOTAQ HS DNA Polymerase Set (#241-08890-92) 241-08890-92 -20 °C 500 reactions 440.00 Buy

Shipping at 4C (#241-08800-98)