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COSMOSIL SL-II

Normal Phase

  • High purity silica gel (>99.99%) with specially treatment
  • Suitable for preparative separation
SL-II
USP code L3
Silica gel High purity porous spherical silica
Average particle size 3 µm, 5 µm, 15 µm
Average pore size approx. 120Å
Specific surface area approx. 300m2/g

Analysis of Acids and Amide without Ionic Additives

Column size: 4.6 mm I.D. x 250 mm
Mobile phase: Methanol : Hexane = 10 : 90
Flow rate: 1 ml/min
Temperature: 30°C
Detection: UV 254 nm, 0.5AUFS
Sample: 1. Benzoic Acid 4.0 µg
2. Salicylic Acid 6.0 µg
3. Salicylamide 4.0 µg

Comparison with Medium-pressure Columns

COSMOSIL SL-II offers sharper peak compared with packing materials for medium-pressure liquid chromatography and open chromatography.
Column 5SL-II A Company Cartridge
(30 µm silica gel)
B Company Cartridge
(60 µm silica gel)
Column Size 20 mm I.D. x 250 mm 26 mm I.D. x 104 mm 26 mm I.D. x 104 mm
Pressure 1.3 MPa 0.25 MPa 0.15 MPa
Chromatogram
Mobile phase: Ethanol : Hexane = 5 : 95
Flow rate: 10 ml/min
Temperature: Room Temperature
Detection: UV 254 nm
Sample: 1. p-Xylene 8.3 mg
2. Cinnamyl Alcohol 1.7 mg
3. p-Nitrobenzyl Alcohol 4.2 mg

Scaling Up from Analytical to Preparative Separation

COSMOSIL SL-II offers sharper peak compared with packing materials for medium-pressure liquid chromatography and open chromatography.
Step 1 Step 2 Step 3
Mobile phase is optimized by using 4.6 mm I.D. x 250 mm column Maximum load capacity is optimized by using 4.6 mm I.D. x 250 mm column Preparative separation by using 20 mm I.D. x 250 mm column
As mobile phase, highly soluble solvent for sample is suitable. Maximum injection volume 56 µl (28 mg) Maximum injection volume 1,050 µl (525 mg) (19 times higher than 4.6 mm I.D.)
Mobile phase: Ethyl Acetate : Hexane = 10 : 90
Flow rate: 4.6 mm I.D. : 1 ml/min
20 mm I.D. : 18.9 ml/min
Temperature: Room Temperature
Detection: UV 254 nm
Sample: 1.1-Naphthol
2.2-Naphthol

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Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 10 mm) 38059-61 Waters type 1 pkg 325.00 Buy
COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 50 mm) 38060-21 Waters type 1 pkg 325.00 Buy
COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 100 mm) 38061-11 Waters type 1 pkg 475.00 Buy
COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 150 mm) 02266-51 Waters type 1 pkg 475.00 Buy
COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 250 mm) 19400-51 Waters type 1 pkg 590.00 Buy
COSMOSIL 3SL-II Packed Column (6.0 mm I.D. x 100 mm) 09192-01 Waters type 1 pkg 785.00 Buy
COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 50 mm) 37999-81 Waters type 1 pkg 325.00 Buy
COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 100 mm) 38000-01 Waters type 1 pkg 440.00 Buy
COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 150 mm) 38001-91 Waters type 1 pkg 400.00 Buy
COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 250 mm) 38002-81 Waters type 1 pkg 440.00 Buy
COSMOSIL 5SL-II Packed Column (6.0 mm I.D. x 150 mm) 38003-71 Waters type 1 pkg 505.00 Buy
COSMOSIL 5SL-II Packed Column (6.0 mm I.D. x 250 mm) 38004-61 Waters type 1 pkg 565.00 Buy
COSMOSIL 5SL-II Packed Column (10.0 mm I.D. x 250 mm) 38005-51 Waters type 1 pkg 905.00 Buy
COSMOSIL 5SL-II Packed Column (20.0 mm I.D. x 250 mm) 38006-41 Waters type 1 pkg 1,900.00 Buy
COSMOSIL 5SL-II Packed Column (28.0 mm I.D. x 250 mm) 34358-61 Waters type 1 pkg 3,800.00 Buy
COSMOSIL 15SL-II Packed Column (28.0 mm I.D. x 250 mm) 05893-41 Waters type 1 pkg 2,930.00 Buy
COSMOSIL 15SL-II Packed Column (50.0 mm I.D. x 250 mm) 05895-21 Waters type 1 pkg 4,640.00 Buy
COSMOSIL 15SL-II Packed Column (50.0 mm I.D. x 500 mm) 05896-11 Waters type 1 pkg 6,970.00 Buy
Guard Column
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 5SL-II Guard Column (4.6 mm I.D. x 10 mm) 37997-01 Waters type 1 pkg 200.00 Buy
COSMOSIL 5SL-II Guard Column (10.0 mm I.D. x 20 mm) 37998-91 Waters type 1 pkg 345.00 Buy
COSMOSIL 5SL-II Guard Column (20.0 mm I.D. x 20 mm) 05874-91 Waters type 1 pkg 755.00 Buy
COSMOSIL 5SL-II Guard Column (20.0 mm I.D. x 50 mm) 05875-81 Waters type 1 pkg 985.00 Buy
COSMOSIL 5SL-II Guard Column (28.0 mm I.D. x 50 mm) 34359-51 Waters type 1 pkg 1,785.00 Buy
COSMOSIL 15SL-II Guard Column (28.0 mm I.D. x 50 mm) 05892-51 Waters type 1 pkg 1,640.00 Buy
COSMOSIL 15SL-II Guard Column (50.0 mm I.D. x 50 mm) 05894-31 Waters type 1 pkg 3,855.00 Buy