Normal Phase
| SL-II | |
|---|---|
| USP code | L3 |
| Silica gel | High purity porous spherical silica |
| Average particle size | 3 µm, 5 µm, 15 µm |
| Average pore size | approx. 120Å |
| Specific surface area | approx. 300m2/g |
| Column size: | 4.6 mm I.D. x 250 mm |
| Mobile phase: | Methanol : Hexane = 10 : 90 |
| Flow rate: | 1 ml/min |
| Temperature: | 30°C |
| Detection: | UV 254 nm, 0.5AUFS |
| Sample: | 1. Benzoic Acid 4.0 µg 2. Salicylic Acid 6.0 µg 3. Salicylamide 4.0 µg |
| Column | 5SL-II | A Company Cartridge (30 µm silica gel) |
B Company Cartridge (60 µm silica gel) |
|---|---|---|---|
| Column Size | 20 mm I.D. x 250 mm | 26 mm I.D. x 104 mm | 26 mm I.D. x 104 mm |
| Pressure | 1.3 MPa | 0.25 MPa | 0.15 MPa |
| Chromatogram |  |
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| Mobile phase: | Ethanol : Hexane = 5 : 95 |
| Flow rate: | 10 ml/min |
| Temperature: | Room Temperature |
| Detection: | UV 254 nm |
| Sample: | 1. p-Xylene 8.3 mg 2. Cinnamyl Alcohol 1.7 mg 3. p-Nitrobenzyl Alcohol 4.2 mg |
| Step 1 | Step 2 | Step 3 |
|---|---|---|
| Mobile phase is optimized by using 4.6 mm I.D. x 250 mm column | Maximum load capacity is optimized by using 4.6 mm I.D. x 250 mm column | Preparative separation by using 20 mm I.D. x 250 mm column |
| As mobile phase, highly soluble solvent for sample is suitable. | Maximum injection volume 56 µl (28 mg) | Maximum injection volume 1,050 µl (525 mg) (19 times higher than 4.6 mm I.D.) |
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| Mobile phase: | Ethyl Acetate : Hexane = 10 : 90 |
| Flow rate: | 4.6 mm I.D. : 1 ml/min 20 mm I.D. : 18.9 ml/min |
| Temperature: | Room Temperature |
| Detection: | UV 254 nm |
| Sample: | 1.1-Naphthol |  |
| 2.2-Naphthol |  |
(0.7 MB)COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 3SL-II Packed Column (2.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
18282-41 | 1 pkg | 557.00 | Buy |
| COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 10 mm) | End-Fitting: Waters type |
38059-61 | 1 pkg | 348.00 | Buy |
| COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 50 mm) | End-Fitting: Waters type |
38060-21 | 1 pkg | 364.00 | Buy |
| COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 100 mm) | End-Fitting: Waters type |
38061-11 | 1 pkg | 450.00 | Buy |
| COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type |
02266-51 | 1 pkg | 509.00 | Buy |
| COSMOSIL 3SL-II Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type |
19400-51 | 1 pkg | 632.00 | Buy |
| COSMOSIL 3SL-II Packed Column (6.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
09192-01 | 1 pkg | 840.00 | Buy |
| COSMOSIL 5SL-II Packed Column (1.0 mm I.D. x 100 mm) | End-Fitting: Waters type |
08978-51 | 1 pkg | 535.00 | Buy |
| COSMOSIL 5SL-II Packed Column (2.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
19547-51 | 1 pkg | 514.00 | Buy |
| COSMOSIL 5SL-II Packed Column (2.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
06945-31 | 1 pkg | 568.00 | Buy |
| COSMOSIL 5SL-II Packed Column (3.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
07380-91 | 1 pkg | 428.00 | Buy |
| COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 50 mm) | End-Fitting: Waters type |
37999-81 | 1 pkg | 348.00 | Buy |
| COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 100 mm) | End-Fitting: Waters type |
38000-01 | 1 pkg | 471.00 | Buy |
| COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 150 mm) | End-Fitting: Waters type |
38001-91 | 1 pkg | 428.00 | Buy |
| COSMOSIL 5SL-II Packed Column (4.6 mm I.D. x 250 mm) | End-Fitting: Waters type |
38002-81 | 1 pkg | 471.00 | Buy |
| COSMOSIL 5SL-II Packed Column (6.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
38003-71 | 1 pkg | 541.00 | Buy |
| COSMOSIL 5SL-II Packed Column (6.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
38004-61 | 1 pkg | 696.00 | Buy |
| COSMOSIL 5SL-II Packed Column (10.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
38005-51 | 1 pkg | 969.00 | Buy |
| COSMOSIL 5SL-II Packed Column (20.0 mm I.D. x 150 mm) | End-Fitting: Waters type |
04668-31 | 1 pkg | 1,819.00 | Buy |
| COSMOSIL 5SL-II Packed Column (20.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
38006-41 | 1 pkg | 2,033.00 | Buy |
| COSMOSIL 5SL-II Packed Column (28.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
34358-61 | 1 pkg | 4,066.00 | Buy |
| COSMOSIL 15SL-II Packed Column (28.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
05893-41 | 1 pkg | 3,136.00 | Buy |
| COSMOSIL 15SL-II Packed Column (50.0 mm I.D. x 250 mm) | End-Fitting: Waters type |
05895-21 | 1 pkg | 4,965.00 | Buy |
| COSMOSIL 15SL-II Packed Column (50.0 mm I.D. x 500 mm) | End-Fitting: Waters type |
05896-11 | 1 pkg | 7,458.00 | Buy |
| Product | Description | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| COSMOSIL 5SL-II Guard Column (4.6 mm I.D. x 10 mm) | End-Fitting: Waters type |
37997-01 | 1 pkg | 214.00 | Buy |
| COSMOSIL 5SL-II Guard Column (10.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
37998-91 | 1 pkg | 370.00 | Buy |
| COSMOSIL 5SL-II Guard Column (20.0 mm I.D. x 20 mm) | End-Fitting: Waters type |
05874-91 | 1 pkg | 808.00 | Buy |
| COSMOSIL 5SL-II Guard Column (20.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
05875-81 | 1 pkg | 1,087.00 | Buy |
| COSMOSIL 5SL-II Guard Column (28.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
34359-51 | 1 pkg | 1,910.00 | Buy |
| COSMOSIL 15SL-II Guard Column (28.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
05892-51 | 1 pkg | 1,755.00 | Buy |
| COSMOSIL 15SL-II Guard Column (50.0 mm I.D. x 50 mm) | End-Fitting: Waters type |
05894-31 | 1 pkg | 4,125.00 | Buy |