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Column Selection Guide

• Organic Compounds (Low M.W.)   
Octadecyl group bonded column (C18, ODS) are recommended as first-choice columns for separations of organic compounds (Low M.W.). If there is not enough separation or no retention using COSMOSIL C18 columns, COSMOSIL series offer many kinds of special columns.
 
• Saccharides
COSMOSIL Sugar-D is recommended for the separation of monosaccharides and oligosaccharides as a first-choice column. For the separation of sugar derivatives, COSMOSIL C18-PAQ is suitable as well.
 
• Proteins
Please select based on the separation mode.
 
• Fullerenes 
COSMOSIL Buckyprep is most suitable for the separation of fullerenes.

Reversed Phase Specialty Columns

Reversed phase HPLC columns have been widely used because of their superior resolution, high theoretical plate number and ease of use. Since hydrophobic interaction is the dominant separation mechanism in reversed phase chromatography, conventional stationary phases such as C18 and C18 do not offer optimum selectivity for compounds with similar hydrophobicity. COSMOSIL offers a broad selection of columns with unique stationary phases for separation of these difficult analytes. These columns offer improved separation of structurally similar compounds that are difficult to analyze with a C18 type column.

Specifications

Packing MaterialCholesterPBrPFPπNAPPYENPE
Silica Gel Fully-Porous Core-Shell Fully-Porous Core-Shell Fully-Porous Fully-Porous Fully-Porous Fully-Porous
Particle Size 2.5 µm 5 µm 2.6 µm 5 µm 2.6 µm 5 µm 2.5 µm 5 µm 5 µm 5 µm
Pore Size 130Å 120Å 90Å 120Å 90Å 120Å 130Å 120Å 120Å 120Å
Specific Surface Area 300 m2/g 300 m2/g 150 m2/g 300 m2/g 150 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g 300 m2/g
Stationary Phase Cholesteryl Group Pentabromobenzyl group Pentafluorophrnyl group  Naphthylethyl
Group
Pyrenylethyl
Group
Nitrophenylethyl
Group
Bonding Type Monomeric
Main Interaction
- Hydrophobic Interaction
- Molecular Shape Selectivity
- Hydrophobic interaction
- Dispersion force
 
- Hydrophobic interaction
- π-π Interaction
- Dipole-dipole Interaction
- Hydrophobic Interaction
- π-π Interaction
- Hydrophobic Interaction
- π-π Interaction
- Dispersion Force
- Molecular shape selectivity
- Hydrophobic Interaction
- π-π Interaction
- Dipole-dipole Interaction
End Capping Treatment Near-perfect treatment
Carbon Content 21% 20% - 8% -  10% 14% 11% 18% 9%
pH Range 2–7.5
Feature
- Usable under the same conditions as C18
- High molecular shape selectivity
- Separate hydrophilic compounds in reversed phase conditions
- Separate using dispersion force
 - Weak dipole-dipole interaction
- Stronger π-π interaction than phenyl column
- Very strong π-π interaction
- Strong dipole-dipole interaction

Fully-Porous: High purity porous spherical silica / Core-Shell: Core-Shell silica gel 

 

Selectivity for positional isomers of dinitrobenzene

Different stationary phase exhibits different selectivity due to the use of forces that C18 (hydrophobic interaction) does not have. By using these columns, you can achieve separation that cannot be done using only C18.

 

 

COSMOSIL Column List by Stationary Phase

COSMOSIL / COSMOGEL Packing Material List

Separation ModePacking MaterialStationary PhaseSpecial Features and ApplicationsUSP Code
Reversed Phase 3C18-EB Octadecyl Group - Excellent for basic compounds
- 3 µm silica packing material
L1
C18-MS-II Octadecyl Group - Multi-purpose C18 column
- Monofunctional silylation on ultra-pure silica gel for separation of the widest range of compounds
L1
C18-AR-II - Features strong acid resistance and suitable for acidic compounds and peptides L1
C18-PAQ - Reversed phase column, compatible with 100% water based mobile phase.
- Suitable for hydrophilic compounds
L1
Cholester Cholesteryl Group - Usable under the same condition as C18
- Unique rigid cholesteryl structure improves separation
L101
PBr Pentabromobenzyl group - Separate hydrohilic compounds in reversed-phase conditions  
πNAP Naphtylethyl Group - Stronger π-π interaction than Phenyl column  
PYE Pyrenylethyl Group - Most powerful π-π interaction  
NPE Nitrophenylethyl Group - Separation utilizing π-π interaction and dipole-dipole interaction  
PFP Pentafluorophenyl Group - Separation by dipole-dipole interactions. L43
CN-MS Cyanopropyl Group - Great reproducibility using isocratic elution mode L10
C22-AR-II Docosyl Group - Alkyl chain columns except C18column  
C8-MS Octyl Group L7
C4-MS Butyl Group L26
PE-MS Phenylethyl Group - π-π interaction L11
Protein-R Octadecyl Group - Provides improved separation, high recovery rate, and acid resistance L1
C18-AR-300 Octadecyl Group - Wide pore type reversed phase columns L1
C8-AR-300 Octyl Group L7
C4-AR-300 Butyl Group L26
Ph-AR-300 Phenyl Group L11
Normal Phase SL-II - - Suitable for preparative separation L3
Hydrophilic
Interaction
HILIC Triazole - Retains highly polar compounds that would not be retained in C18column L104
Sugar-D Secondary/Tertiary Amine - A novel stationaly phase for mono- and oligosaccharides  
NH2-MS Aminopropyl Group - Primary amine bonded column L8

Gel
Permeation (Silica-based)

Diol-120-II Diol Group - Sample MW: (Protein) 5,000-100,000 Da, (Water-Soluble Polyer) 1,000-20,000 L20
Diol-300-II - Sample MW (Protein) 10,000-700,000 Da,  (Water-Soluble Polyer) 5,000-100,000
Diol-1000-II - Sample MW (Water Soluble Polymer) 50,000 - 500,000 Da
Ion-exchange Type Q Quaternary Ammonium Type - Anion-exchange Type (for purification)
- Target Sample: Acidic Proteins / DNA
 
Type Q-N - Anion-exchange Type (for Ultra-fast Analysis, Precise Analysis)
- Target Sample: Acidic Proteins / DNA
 
Type S Sulfopropyl Type - Cation-exchange Type (for Purification)
- Target Sample: Basic Proteins
 
Type S-N - Cation-exchange Type (for Ultra-fast Analysis, Precise Analysis)
- Target Sample: Basic Proteins
 
Type M Quaternary Ammonium Type/ Sulfopropyl Type - Amphoteric ion-exchange Type (for Purification)
- Target Sample: All Proteins
 
Type M-N - Amphoteric ion-exchange Type (for Precise Analysis)
- Target Sample: All Proteins
 
Hydrophobic
Interaction
HIC - - Suitable for separation of peptide, and there is only a little less in enzyme activity and the teriary structure of proteins  
- Buckyprep Pyrenylpropyl Group - Standard column for fullerene separation  
Buckyprep- D Nitro-carbazoyl Group - Designed for separation of fullerene derivatives  
Buckyprep-M Phenothiazinyl Group - Designed to separate metallofullerenes  
PBB Pentabromobenzyl Group - Designed for preparative separation of C60, C70  
PYE Pyrenylethyl Group - Separation of Fullerenes and structural isomers  
NPE Nitrophenylethyl Group - Separation of derivatived fullerene  
Gel
Permeation
CNT-300 Hydrophilic Group (neutral) - Separation of soluble carbon nanotubes  
CNT-1000  
CNT-2000  
Chiral Separation CHiRAL A Amylose tris (3,5-dimethylphenyl
carbamate)
- Immobilized selectors can withstand many different solvents L99
CHiRAL B Cellulose tris (3,5-dimethylphenyl
carbamate)
 
CHiRAL C Cellulose tris (3,5-dichlorophenyl
carbamate)
L119

 

COSMOSIL SFC Column List

Separation ModePacking MaterialStationary PhaseSpecial Features and ApplicationsUSP Code
SFC PY Pyridinyl Group - Similar separation properties as 2-Ethylpyridine, with stronger retention.  
HP 3-Hydroxyphenyl Group
- Good for hydrophilic compounds.
- Stronger retention for basic compounds than PY.
 
Diol Diol Group - Less effect from ionic interaction.  
Cholester Cholesteryl Group - Longer retention and better separation than C18.  
πMAX Pyrenylethyl Group - Stronger π-π interaction than phenyl column.  
PBr Pentabromobenzyl group - Unique separations using dispersion force.  

 

Column Selection Guide by Sample Type

SampleCategorySeparation ModeRecommended ColumnRemark
Low-MW drugs - Reversed phase C18-EB Near perfect end capping treatment
COSMOCORE C18
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Normal phase SL-II Standard for normal phase
Vitamins Water-soluble vitamins Reversed phase C18-PAQ Compatible with 100% water based mobile phase
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Fat-soluble vitamins Reversed phase C18-MS-II Standard for reversed phase
Cholester Different selectivity from C18
Normal phase SL-II Standard for normal phase
Natural products - Reversed phase C18-MS-II Utilize various interactions for versatile separations. See each product page for details.
Cholester
PBr
πNAP
Normal phase SL-II Suitable for preparative separation
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Organic acids - Reversed phase C18-PAQ Compatible with 100% water based mobile phase
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Fatty acids - Reversed phase C18-AR-II Features strong acid resistance
Cholester Different selectivity from C18
Phospholipids Molecular species Reversed phase C18-MS-II Standard for reversed phase
Class species Normal phase SL-II Standard for normal phase
Agricultural chemicals - Reversed phase C18-MS-II Standard for reversed phase
Cholester Different selectivity from C18
Normal phase SL-II Standard for normal phase
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Metabolites - Reversed phase C18-MS-II Standard for reversed phase
Cholester Different selectivity from C18
Normal phase SL-II Standard for normal phase
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Food additives - Reversed phase C18-MS-II Standard for reversed phase
Cholester Different selectivity from C18
Normal phase SL-II Standard for normal phase
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Other low-MW compounds - Reversed phase C18-MS-II Standard for reversed phase
Cholester Different selectivity from C18
Normal phase SL-II Standard for normal phase
Hydrophilic interaction HILIC Retains highly polar compounds that would not be retained in C18 column
Structural isomers
Structural analogs 
Reversed phase C18-MS-II Utilize various interactions for versatile separations. See each product page for details.
C18-AR-II
Cholester
πNAP
PYE
NPE
PBr
PFP
Normal phase SL-II Standard for normal phase
Optical isomers - Normal phase /Reversed phase CHiRAL A 3 chiral selectors with high overall hit rate
CHiRAL B
CHiRAL C
Amino acids Free amino acids Reversed phase PBr Retains aromatic amino acids
Hydrophilic interaction HILIC For amino acids not retained in reversedphase mode
Labeled amino acids Reversed phase C18-AR-II Features strong acid resistance
Peptides
Proteins
MW 3,000 or less Reversed phase C18-AR-II Features strong acid resistance
PBr Separation for oligopeptides
Hydrophilic interaction HILIC For hydrophilic peptides not retained in reversed-phase mode
MW 3,000 or more Reversed phase Protein-R Wide pore columns
C18-AR-300
C4-AR-300
Size exclusion Diol-II Separation utilizing molecular size
Ion exchange IEX Separation based on charge
Nucleic acids Nucleic acid bases Reversed phase PBr Separate under reversed-phase condition
Hydrophilic interaction HILIC Different selectivity from reversed phase
Nucleosides
Nucleotides
Reversed phase C18-PAQ Compatible with 100% water based mobile phase
PBr Strong retain than C18
Hydrophilic interaction HILIC Different separatin from reversed phase
Oligonucleotides Reversed phase C18-MS-II Standard for reversed phase
Size exclusion RNA-SEC Separation utilizing molecular size
Ion exchange IEX Separation based on charge
Sugars Monosaccharides Hydrophilic interaction Sugar-D Separation in non-derivatized form
NH2-MS
Labeled saccharides Reversed phase C18-PAQ For pyridylaminated sugars
Hydrophilic interaction Sugar-D For two-dimensional separations with reversed-phase
NH2-MS
Oligosaccharides Reversed phase PBr Retained in reversed-phase mode
Hydrophilic interaction Sugar-D Separation in non-derivatized form
NH2-MS
Polysaccharides Size exclusion Diol-II Separation utilizing molecular size
Fullerenes Fullerenes - Buckyprep Standard for fullerene separation
Metallofullerenes - Buckyprep Different selectivity for metallofullerenes
Buckyprep-M
Derivatized fullerenes - Buckyprep Separation in toluene mobile phase
Buckyprep-D
Carbon nanotubes - Size exclusion  CNT Separation of soluble carbon nanotubes
Water-soluble polymer Size exclusion Diol-II Separation utilizing molecular size

 

Columns for USP Specifications

COSMOSIL offers a comprehensive range of HPLC columns which meet the USP (United States Pharmacopoeia) specifications.
USP CodeDescriptionRecommended Columns
L1 Octadecyl silane chemically bonded to porous silica or ceramic micro-particles, 1.7 to 10 m in diameter, or a monolithic rod COSMOSIL 3C18EB
COSMOSIL C18-MS-II
COSMOSIL C18-AR-II
COSMOSIL C18-PAQ
COSMOSIL C18-AR-300
COSMOSIL Protein-R
L3 Porous silica particles, 5 to 10 µm in diameter COSMOSIL SL-II
L7 Octylsilane chemically bonded to totally porous silica particles, 1.7 to 10 µm in diameter COSMOSIL C8-MS
COSMOSIL C8-AR-300
L8 An essentially monomolecular layer of aminopropylsilane chemically bonded to totally porous silica gel support, 1.5 to 10 µm in diameter, or a monolithic silica rod. COSMOSIL NH2-MS
L10 Nitrile groups chemically bonded to porous silica particles, 3 to 10 µm in diameter COSMOSIL CN-MS
L11 Phenyl groups chemically bonded to porous silica particles, 1.7 to 10 µm in diameter COSMOSIL PE-MS
COSMOSIL Ph-AR-300
L13 Trimethylsilane chemically bonded to porous silica particles, 3 to 10 µm in diamete COSMOSIL TMS-MS
L20 Dihydroxypropane groups chemically bonded to porous silica particles, 5 to 10 µm in diameter COSMOSIL Diol-120-II
COSMOSIL Diol-300-II
COSMOSIL Diol-1000-II
L26 Butyl silane chemically bonded to totally porous silica particles, 3 to 10 µm in diameter COSMOSIL C4-MS
COSMOSIL C4-AR-300
L43 Pentafluorophenyl groups chemically bonded to silica particles by a propyl spacer, 1.5 to 10 µm in diameter COSMOSIL PFP
L99 Amylose tris-(3,5-dumethylphenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter COSMOSIL CHiRAL A
L101 Cholesteryl groups chemically bonded to porous or non-porous silica or ceramic micro-particles, 1.5 to 10 µm in diameter, or a monolithic rod. COSMOSIL Cholester
COSMOCORE Cholester
L104 Triazole groups chemically bonded to porous silica particles, 1.5 to 10 µm in diameter COSMOSIL HILIC
L119 Cellulose tris-(3,5-dichlorophenylcarbamate), immobilized on porous, spherical, silica particles, 3 to 5 µm in diameter COSMOSIL CHiRAL C

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size