Nacalai USA - Innovations for Life Sciences

Subtotal: $0.00

View Cart

Cell Count Reagent SF

Cell Count Reagent SF allows sensitive colorimetric assays by utilizing highly water-soluble tetrazolium salt. WST-8 produces a water-soluble formazan dye upon reduction in the presence of an electron carrier. Since the absorbance at 450 nm is proportional to the number of viable cells in the medium, the viable cell number can be determined using the absorbance value of a previously prepared calibration curve. This product is a one-bottle solution; no premixing of components is required.

Features:

  • No radioisotopes
  • No solubilization steps for formazan
  • More sensitive than other water-soluble tetrazolium salts (XTT, MTS)
  • Ready to use
  • More stable than other kits

Product information

Principle

 

Comparison of Assay Procedure with MTT and Cell Count Reagent SF

 

Absorption spectrum of WST-8 formazan

Applications

References

  • M. Ishiyama, Y. Miyazono, K. Sasamoto, Y. Ohkura, K. Ueno, Talanta, 44, 1299 (1997)

  • H. Tominaga, M.Ishiyama, F. Ohseto, K. Sasamoto, T. Hamamoto, K. Suzuki and M. Watanabe, Anal. Commun, 36 (2), 47 (1999

 

  • Nakano, Dan, et al. "Effects of canagliflozin on growth and metabolic reprograming in hepatocellular carcinoma cells: Multi-omics analysis of metabolomics and absolute quantification proteomics (iMPAQT)." PloS one 15.4 (2020): e0232283.

  • Kuroda, Kosuke, et al. "Non-aqueous, zwitterionic solvent as an alternative for dimethyl sulfoxide in the life sciences." Communications Chemistry 3.1 (2020): 1-7.

  • Aikawa, Akiyoshi, et al. "Cell death induced by dorsomorphin in adult T‐cell leukemia/lymphoma is AMPK‐independent." The FEBS journal 287.18 (2020): 4005-4015.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Cell Count Reagent SF - 500 tests 07553-15 4°C 5 mL 136.00 Buy
Cell Count Reagent SF - 2500 tests 07553-44 4°C 5 x 5 mL 378.00 Buy