Cell Reservoir One
Serum-Free Cell Freezing Medium
Cell Reservoir One is a serum-free cell culture freezing medium, which contains a water-soluble glycoprotein SERICIN isolated from the silkworm cocoon as a major constituent. SERICIN shows the same high efficacy of cryopreservation as FBS, and reduces the cell toxicity of DMSO. As DMSO is known to have adverse effects on cellular functions, especially Embryonic Stem Cells, Cell Reservoir One is available both with and without DMSO.
Application 1
Mouse ES cells:Viability after thawing
Mouse ES cells: Proliferative ability and undifferentiated state (3 days after thawing)
Mouse ES cells: Differentiation into Beating cardiomyocytes
Mouse ES cells: Freezing media affect the differentiation
Application 2
Human Mesenchymal Stem Cell (hMSC): Bone differentiation
Application 3
Pancreatic islet transplantation model
Bioartificial liver model
Cell viabilities in Cell Reservoir One with DMSO and without DMSO
| Animal Species | Cell Species | Derivation | Freezing Period | Viability rate (%) | |
|---|---|---|---|---|---|
| with DMSO | without DMSO | ||||
| Human | Jurkat | Blood | 1 year | 90 | 85 |
| Human | HL-60 | Blood | 1 year | 90 | 80 |
| Human | WIL2-NS | Spleen | 1 year | 90 | 70 |
| Human | MSC | Bone Marrow | 1 year | 90 | 70 |
| Human | HeLa | Uterine Cervix | 1 year | 90 | 80 |
| Human | HepG2 | Liver | 1 year | 85 | 70 |
| Human | NHDF | Normal Dermis | 1 year | 90 | 75 |
| Mouse | KUSA-A1 | Bone Marrow | 9 months | 90 | 80 |
| Mouse | 2E3-O | Hybridoma | 1 year | 90 | 80 |
| Mouse | P3U1 | Bone Marrow | 1 year | 90 | 80 |
| Mouse | OP9 | Skullcap | 10 months | 95 | 85 |
| Mouse | ES | Embryo | 1 year | 80 | 90 |
| Rat | PC-12 | Adrenal Gland | 1 year | 90 | 70 |
| Hamster | CHO | Ovary | 1 year | 95 | 90 |
| Rabbit | RC4 | Cornea | 1 year | 90 | 70 |
| Insect | Sf-9 | Ovary | 1 year | 75 | 70 |
Protocol
[Freezing]
- Collect culture cells at their logarithmic growth phase.
- Suspend 5x105 -1x107 cells in 1 ml of Cell Reservoir One, and dispense the cell suspension in cryogenic vials.
- Freeze the cells at -80°C without preliminary freezing. The cells frozen at -80°C can be subsequently preserved in liquid nitrogen.
[Recovery]
- Remove the frozen vial from cryopreservation storage and rapidly thaw it in a 37°C water bath.
- Gently dilute the cells with cell culture medium by mixing.
- Centrifuge the cell suspension and remove the supernatant*, and resuspend the cells in culture medium.
*Centrifugation step may be omitted in the case Cell Reservoir One without DMSO is used for ES cells.
Downloads
Cell Reservoir One (with DMSO) (Instruction)Reference
- Aoki, Hiromasa, et al. "Efficient differentiation and purification of human induced pluripotent stem cell-derived endothelial progenitor cells and expansion with the use of inhibitors of ROCK, TGF-β, and GSK3β." Heliyon 6.3 (2020): e03493.
- Sawada, Ryoichi, et al. "High blood levels of soluble OX40 (CD134), an immune costimulatory molecule, indicate reduced survival in patients with advanced colorectal cancer." Oncology reports 42.5 (2019): 2057-2064.