This product is a red to red-yellow crystal or powder that dissolves in acetone. It is a labeling reagent for amino groups and includes a chiral carbon, enabling the separation of labeled amino acids with a C18 or other achiral column (Figure. 1). As the reagent itself is more easily ionizable compared to conventional products, higher sensitivity with LC-MS can be achieved (Figure. 2), and it is suitable for analyzing trace amounts of peptides.
Figure. 1: Chiral separations of L-FDVDA-labeled amino acids
Figure. 2: Comparison of detection sensitivity using LC-MS
Data provided by Dr. Takefumi Kuranaga, Department of System Chemotherapy and Molecular Sciences, Division of Bioinformatics and Chemical Genomics, Graduate School of Pharmaceutical Sciences, Kyoto University
Kuranaga, Takefumi, et al. "Highly sensitive labeling reagents for scarce natural products." ACS Chemical Biology 15.9 (2020): 2499-2506.
Pan, Chengqian, et al. "Amycolapeptins A and B, cyclic nonadepsipeptides produced by combined-culture of Amycolatopsis sp. and Tsukamurella pulmonis." The Journal of Organic Chemistry 86.2 (2021): 1843-1849.
Morimoto, Ryota, et al. "Highly Sensitive Determination of Amino Acids by LC-MS under Neutral Conditions." Chemical and Pharmaceutical Bulletin 69.3 (2021): 265-270.
Jiang, Yulu, et al. "Longicatenamides A–D, Two Diastereomeric Pairs of Cyclic Hexapeptides Produced by Combined-culture of Streptomyces sp. KUSC_F05 and Tsukamurella pulmonis TP-B0596." The Journal of Antibiotics 74.5 (2021): 307-316.
Pan, Chengqian, Takefumi Kuranaga, and Hideaki Kakeya. "Application of the highly sensitive labeling reagent to the structural confirmation of readily isomerizable peptides." Journal of natural medicines 75.2 (2021): 339-343.
|Product||Cat.No.||Formula = M.W.||Purity||Storage||PKG Size||Price(US$)|
|L-FDVDA (1-Fluoro-2,4-dinitrophenyl-5-L-valine-N,N-dimethylethylenediamine-amide)||20363-24||C15H22N5O5F = 371.36||98.0% or more (HPLC)||4℃||50 mg||200.00||Buy|
The labeling reagent ispatented by Kyoto University. Nacalai Tesque, Inc. produces and distributes this product