Nacalai USA - Innovations for Life Sciences

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CosmoPAGE Precast Gel

Cost-effective, Compatible with Bio-Rad Mini-PROTEAN

CosmoPAGE TG Precast Gels (Tris-Glycine) are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, CosmoPAGE TG Precast Gels perform enhanced resolution, sharper bands, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in CosmoPAGE TG series, however, are similar with typical Laemmli Tris-HCl gels, and thus CosmoPAGE TG Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses. CosmoPAGE TG Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 15-well formats.

CosmoPAGE Bis-Tris Precast Gels are a high-performance and easy to use precast polyacrylamide gels for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows CosmoPAGE Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional gels. CosmoPAGE Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (12%) concentrations of polyacrylamide in 15-well formats.

 

Features
  • Compatible with Bio-Rad Mini-Protean Core
  • Consistent performance
  • Sharp, Straight bands
  • Long shelf life: Up to 12 months

 

Cassette size:  Mini Gel (10 x 8.3 cm)
Gel dimensions:  8.1(w) x 7.4(L) x 0.1 cm 
Well format:  15-well
Capacity:  25 µl/well

Consistent Performance

CosmoPAGE gels perform high protein resolution and reproducibility even stored at ambient temperature for 50 days.

(Condition)
Gel : CosmoPAGE TG Gel 4-15 %
Voltage: 150V for an hour
Sample:
   Lane 1. Prestained Protein Ladder, Broad Range (Cat. No.: NU01002), 3 μl
   Lane 2. 3. E. coli lysate, 10 μg
   Lane 4. 5. Hek293T lysate, 10 μg
   Lane 6. 7. Mouse brain membrane 10 μg
   Lane 8. 9. Mouse brain cytosol 10 μg

Compatibility

Compatible with Bio-rad Mini-PROTEAN® Electrophoresis System without use of extra adapters.

Transfer efficiency

(Conditions)  
SDS-PAGE: 100V, 110 min run time
Transfer: 150 V (300 mA final), 70 min run time
Membrane: Nitrocellulose membrane
Sample: Prestained Protein Ladder, Broad Range(NU01002)
  1. 1 µl
2. 3 µl
3. 5 µl
4. 7 µl
5. 9 µl



 
 

Protein Migration Chart

Buffer recipes

2X Sample Buffer with Reducing Agent

62.5 mM Tris-HCl (pH 6.8), 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)

 

10X Tris-Glycine Running Buffer

Dissolve 30.0 g Tris base, 144.0 g Glycine and 10.0 g SDS in 1000 ml of ddH2O.

 

10X MOPS Running Buffer

Dissolve 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

 

10X MES Running Buffer

Dissolve 60.6 g Tris base, 97.6 g MES, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

 

1X Running Buffer

Dilute 100 ml 10X running buffer with 900 ml ddH2O.

 

10X Transfer Buffer

Dissolve 30.0 g Tris base and 144.0 g Glycine in 1000 ml of ddH2O.

 

1X Transfer Buffer

Cool 1X transfer buffer to 4°C before using.

Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.

Note: For the proper high molecular weight proteins, up to 0.1% SDS may need to be added to 1X transfer buffer.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
CosmoPAGE TG (Tris-Glycine) Precast Gel 10 %, 15 wells NU001015 4°C 10 gels 89.00 Buy
CosmoPAGE TG (Tris-Glycine) Precast Gel 4-15 %, 15 wells NU041515 4°C 10 gels 89.00 Buy
CosmoPAGE Bis-Tris Precast Gel 12 %, 15 wells NU001215 4°C 10 gels 89.00 Buy
CosmoPAGE Bis-Tris Precast Gel 4-12 %, 15 wells NU041215 4°C 10 gels 89.00 Buy
Running Buffer Solution(10x) for SDS-PAGE, Tris-Glycine 30329-61 Room Temp. 1 L 60.00 Buy
Running Buffer Solution(10x) for SDS-PAGE, Tris-Glycine 30329-74 Room Temp. 5 L 220.00 Buy
CosmoPAGE MOPS Running Buffer, Powder NU01004 Room Temp. 5 x 1L 25.00 Buy

Shipping at room temperature