CosmoPAGE Precast Gel ★Special Promotion★
Compatible with Bio-Rad Mini-PROTEAN
Features
- Consistent performance
- Compatible with Bio-Rad Mini-PROTEAN® Electrophoresis system
-
Long shelf life; Up to 12 months
- Sharp, straight bands
- Tris-glycine and bis-tris gels available
Special Promotion!
The following items are available at discount prices:
- CosmoPAGE TG (Tris-Glycine) Precast Gel 10 %, 15 wells (Cat#: NU001015): Current price $89.00 ⇒ Special price $62.30
- CosmoPAGE TG (Tris-Glycine) Precast Gel 4-15 %, 15 wells (Cat#: NU041515): Current price $89.00 ⇒ Special price $62.30
- CosmoPAGE Bis-Tris Precast Gel 12 %, 15 wells (Cat#: NU001215): Current price $89.00 ⇒ Special price $62.30
- CosmoPAGE Bis-Tris Precast Gel 4-12 %, 15 wells (Cat#: NU041215): Current price $89.00 ⇒ Special price $62.30
Consistent Performance
CosmoPAGE gels perform high protein resolution and reproducibility even stored at ambient temperature for 50 days.
CosmoPAGE TG Gel 4-15 %
150V for an hour
1. Prestained Protein Ladder, Broad Range(NU01002), 3 μl
2. E. coli lysate, 10 μg
3. E. coli lysate, 10 μg
4. Hek293T lysate, 10 μg
5. Hek293T lysate, 10 μg
6. Mouse brain membrane 10 μg
7. Mouse brain membrane 10 μg
8. Mouse brain cytosol 10 μg
9. Mouse brain cytosol 10 μg
Compatibility
Compatible with Bio-rad Mini-PROTEAN® Electrophoresis System without use of extra adapters.
Protein Migration Chart
Transfer efficiency
SDS-PAGE: 100V, 110 min run time
Transfer: 150 V (300 mA final), 70 min run time
Membrane: Nitrocellulose membrane
Prestained Protein Ladder, Broad Range(NU01002)
1. 1 ul
2. 3 ul
3. 5 ul
4. 7 ul
5. 9 ul
Instruction
Buffer recipes
2X Sample Buffer with Reducing Agent
62.5 mM Tris-HCl (pH 6.8), 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)
10X Tris-Glycine Running Buffer
Dissolve 30.0 g Tris base, 144.0 g Glycine and 10.0 g SDS in 1000 ml of ddH2O.
10X MOPS Running Buffer
Dissolve 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.
10X MES Running Buffer
Dissolve 60.6 g Tris base, 97.6 g MES, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.
1X Running Buffer
Dilute 100 ml 10X running buffer with 900 ml ddH2O.
10X Transfer Buffer
Dissolve 30.0 g Tris base and 144.0 g Glycine in 1000 ml of ddH2O.
1X Transfer Buffer
Cool 1X transfer buffer to 4°C before using.
Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.
Note: For the proper high molecular weight proteins, up to 0.1% SDS may need to be added to 1X transfer buffer.
Publication
Varki Lab @UC San Diego: Hane M et al. Human-specific microglial Siglec-11 transcript variant has the potential to affect polysialic acid-mediated brain functions at a distance. Glycobiology, 2020. « « NEW!
Iwasaki Lab @Osaka University: Hashizume M et al. Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro. Viruses, 2021. « « NEW!
Ordering Information
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| CosmoPAGE TG (Tris-Glycine) Precast Gel 10 %, 15 wells | NU001015 | 4°C | 10 gels | 62.30 | Buy |
| CosmoPAGE TG (Tris-Glycine) Precast Gel 4-15 %, 15 wells | NU041515 | 4°C | 10 gels | 62.30 | Buy |
| CosmoPAGE Bis-Tris Precast Gel 12 %, 15 wells | NU001215 | 4°C | 10 gels | 62.30 | Buy |
| CosmoPAGE Bis-Tris Precast Gel 4-12 %, 15 wells | NU041215 | 4°C | 10 gels | 62.30 | Buy |
| Running Buffer Solution(10x) for SDS-PAGE, Tris-Glycine | 30329-61 | Room Temp. | 1 L | 60.00 | Buy |
| CosmoPAGE MOPS Running Buffer, Powder | NU01004 | Room Temp. | 5 x 1L | 25.00 | Buy |