Nacalai USA - Innovations for Life Sciences

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CosmoPAGE Precast Gel ★Special Promotion★

Compatible with Bio-Rad Mini-PROTEAN

 Features

  • Consistent performance
  • Compatible with Bio-Rad Mini-PROTEAN® Electrophoresis system
  • Long shelf life; Up to 12 months
  • Sharp, straight bands
  • Tris-glycine and bis-tris gels available

     

Special Promotion!

The following items are available at discount prices:

  • CosmoPAGE TG (Tris-Glycine) Precast Gel 10 %, 15 wells (Cat#: NU001015): Current price $89.00 ⇒ Special price $62.30
  • CosmoPAGE TG (Tris-Glycine) Precast Gel 4-15 %, 15 wells (Cat#: NU041515): Current price $89.00 ⇒ Special price $62.30
  • CosmoPAGE Bis-Tris Precast Gel 12 %, 15 wells (Cat#: NU001215): Current price $89.00 ⇒ Special price $62.30
  • CosmoPAGE Bis-Tris Precast Gel 4-12 %, 15 wells (Cat#: NU041215): Current price $89.00 ⇒ Special price $62.30

Consistent Performance

CosmoPAGE gels perform high protein resolution and reproducibility even stored at ambient temperature for 50 days.

CosmoPAGE TG Gel 4-15 %

150V for an hour
1. Prestained Protein Ladder, Broad Range(NU01002), 3 μl
2. E. coli lysate, 10 μg
3. E. coli lysate, 10 μg
4. Hek293T lysate, 10 μg
5. Hek293T lysate, 10 μg
6. Mouse brain membrane 10 μg
7. Mouse brain membrane 10 μg
8. Mouse brain cytosol 10 μg
9. Mouse brain cytosol 10 μg

Compatibility

Compatible with Bio-rad Mini-PROTEAN® Electrophoresis System without use of extra adapters.

Protein Migration Chart

Transfer efficiency

SDS-PAGE: 100V, 110 min run time
Transfer: 150 V (300 mA final), 70 min run time
Membrane: Nitrocellulose membrane

Prestained Protein Ladder, Broad Range(NU01002)
1. 1 ul
2. 3 ul
3. 5 ul
4. 7 ul
5. 9 ul

Buffer recipes

2X Sample Buffer with Reducing Agent

62.5 mM Tris-HCl (pH 6.8), 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)

 

10X Tris-Glycine Running Buffer

Dissolve 30.0 g Tris base, 144.0 g Glycine and 10.0 g SDS in 1000 ml of ddH2O.

 

10X MOPS Running Buffer

Dissolve 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

 

10X MES Running Buffer

Dissolve 60.6 g Tris base, 97.6 g MES, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

 

1X Running Buffer

Dilute 100 ml 10X running buffer with 900 ml ddH2O.

 

10X Transfer Buffer

Dissolve 30.0 g Tris base and 144.0 g Glycine in 1000 ml of ddH2O.

 

1X Transfer Buffer

Cool 1X transfer buffer to 4°C before using.

Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.

Note: For the proper high molecular weight proteins, up to 0.1% SDS may need to be added to 1X transfer buffer.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
CosmoPAGE TG (Tris-Glycine) Precast Gel 10 %, 15 wells NU001015 4°C 10 gels 62.30 Buy
CosmoPAGE TG (Tris-Glycine) Precast Gel 4-15 %, 15 wells NU041515 4°C 10 gels 62.30 Buy
CosmoPAGE Bis-Tris Precast Gel 12 %, 15 wells NU001215 4°C 10 gels 62.30 Buy
CosmoPAGE Bis-Tris Precast Gel 4-12 %, 15 wells NU041215 4°C 10 gels 62.30 Buy
Running Buffer Solution(10x) for SDS-PAGE, Tris-Glycine 30329-61 Room Temp. 1 L 60.00 Buy
CosmoPAGE MOPS Running Buffer, Powder NU01004 Room Temp. 5 x 1L 25.00 Buy