Cost-effective, Compatible with Bio-Rad Mini-PROTEAN
CosmoPAGE TG Precast Gels (Tris-Glycine) are ready-to-use acrylamide gels for SDS-PAGE running in Tris-Glycine buffer system. With unique formula, CosmoPAGE TG Precast Gels perform enhanced resolution, sharper bands, and longer shelf life as compared with conventional Laemmli Tris-HCl gels. The protein migration patterns in CosmoPAGE TG series, however, are similar with typical Laemmli Tris-HCl gels, and thus CosmoPAGE TG Precast Gels are compatible to traditional SDS-PAGE and subsequent analyses. CosmoPAGE TG Precast Gels are available in gradient (4 to 15%) and fixed (10%) concentrations of polyacrylamide in 15-well formats.
CosmoPAGE Bis-Tris Precast Gels are a high-performance and easy to use precast polyacrylamide gels for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows CosmoPAGE Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional gels. CosmoPAGE Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (12%) concentrations of polyacrylamide in 15-well formats.
The following items are available at discount prices:
*End Date: 4/30/2023
CosmoPAGE gels perform high protein resolution and reproducibility even stored at ambient temperature for 50 days.
Compatible with Bio-rad Mini-PROTEAN® Electrophoresis System without use of extra adapters.
2X Sample Buffer with Reducing Agent
62.5 mM Tris-HCl (pH 6.8), 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)
10X Tris-Glycine Running Buffer
Dissolve 30.0 g Tris base, 144.0 g Glycine and 10.0 g SDS in 1000 ml of ddH2O.
10X MOPS Running Buffer
Dissolve 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.
10X MES Running Buffer
Dissolve 60.6 g Tris base, 97.6 g MES, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.
1X Running Buffer
Dilute 100 ml 10X running buffer with 900 ml ddH2O.
10X Transfer Buffer
Dissolve 30.0 g Tris base and 144.0 g Glycine in 1000 ml of ddH2O.
1X Transfer Buffer
Cool 1X transfer buffer to 4°C before using.
Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.
Note: For the proper high molecular weight proteins, up to 0.1% SDS may need to be added to 1X transfer buffer.
Tips for using CosmoPAGE
|1. Before sample loading, rinse the wells to remove storage buffer.|
|2. Ensure tight-sealing of inner chamber when adaption (or fill outer chamber with buffer to the highest level.)|
|3. Under water flow, insertgel remover between gel and cassette from well site of gel. Slowly push gel remover to the bottom of gel until gel is detached from cassette. * Avoid diagonally peeling the gel from the corner or peeling from the bottm.|
Tips for Western transfer
|1. Assemble transfer sandwich by orientating cathode, sponge, 3 filter papers, gel, membrane, 3 filter papers, sponge, and anode. (The protein goes to the direction of cathode to anode.)|
|2. Remove air bubbles or excess buffer between gel and membrane by carefully moving roller over the membrane.|
|3. Maintain whole transfer process at low temperature. (Keep transfer tank in cold toom or on ice.)|
Varki Lab @UC San Diego: Hane M et al. Human-specific microglial Siglec-11 transcript variant has the potential to affect polysialic acid-mediated brain functions at a distance. Glycobiology, 2020. « « NEW!
Iwasaki Lab @Osaka University: Hashizume M et al. Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro. Viruses, 2021. « « NEW!
|CosmoPAGE TG (Tris-Glycine) Precast Gel 10 %, 15 wells||NU001015||4°C||10 gels||89.00||Buy|
|CosmoPAGE TG (Tris-Glycine) Precast Gel 4-15 %, 15 wells||NU041515||4°C||10 gels||89.00||Buy|
|CosmoPAGE Bis-Tris Precast Gel 12 %, 15 wells||NU001215||4°C||10 gels||89.00||Buy|
|CosmoPAGE Bis-Tris Precast Gel 4-12 %, 15 wells||NU041215||4°C||10 gels||89.00||Buy|
|CosmoPAGE MOPS Running Buffer, Powder||NU01004||Room Temp.||5 x 1L||25.00||Buy|
|CosmoPAGE Tris-Glycine Running Buffer, Powder||NU01011||Room Temp.||5 x 1L||25.00||Buy|
Shipping at room temperature