CosmoPAGE gels perform high protein resolution and reproducibility even stored at ambient temperature for 50 days.

CosmoPAGE TG Gel 4-15 %

150V for an hour
1. Prestained Protein Ladder, Broad Range(NU01002), 3 μl
2. E. coli lysate, 10 μg
3. E. coli lysate, 10 μg
4. Hek293T lysate, 10 μg
5. Hek293T lysate, 10 μg
6. Mouse brain membrane 10 μg
7. Mouse brain membrane 10 μg
8. Mouse brain cytosol 10 μg
9. Mouse brain cytosol 10 μg

Compatible with Bio-rad Mini-PROTEAN^® Electrophoresis System without use of extra adapters.

SDS-PAGE: 100V, 110 min run time
Transfer: 150 V (300 mA final), 70 min run time
Membrane: Nitrocellulose membrane

Prestained Protein Ladder, Broad Range(NU01002)
1. 1 ul
2. 3 ul
3. 5 ul
4. 7 ul
5. 9 ul

CosmoPAGE Bis-tris Precast Gel 15-wells (PDF)

CosmoPAGE Tris-glycine Precast Gel, 15-wells (PDF)

2X Sample Buffer with Reducing Agent

62.5 mM Tris-HCl (pH 6.8), 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)

10X Tris-Glycine Running Buffer

Dissolve 30.0 g Tris base, 144.0 g Glycine and 10.0 g SDS in 1000 ml of ddH2O.

10X MOPS Running Buffer

Dissolve 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

10X MES Running Buffer

Dissolve 60.6 g Tris base, 97.6 g MES, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

1X Running Buffer

Dilute 100 ml 10X running buffer with 900 ml ddH2O.

10X Transfer Buffer

Dissolve 30.0 g Tris base and 144.0 g Glycine in 1000 ml of ddH2O.

1X Transfer Buffer

Cool 1X transfer buffer to 4°C before using.

Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.

Note: For the proper high molecular weight proteins, up to 0.1% SDS may need to be added to 1X transfer buffer.

Varki Lab @UC San Diego: Hane M et al. Human-specific microglial Siglec-11 transcript variant has the potential to affect polysialic acid-mediated brain functions at a distance. Glycobiology, 2020. « « NEW!

Iwasaki Lab @Osaka University: Hashizume M et al. Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro. Viruses, 2021. « « NEW!