CosmoPAGE gels perform high protein resolution and reproducibility even stored at ambient temperature for 50 days.

Compatible with Bio-rad Mini-PROTEAN^® Electrophoresis System without use of extra adapters.

2X Sample Buffer with Reducing Agent

62.5 mM Tris-HCl (pH 6.8), 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)

10X Tris-Glycine Running Buffer

Dissolve 30.0 g Tris base, 144.0 g Glycine and 10.0 g SDS in 1000 ml of ddH2O.

10X MOPS Running Buffer

Dissolve 60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

10X MES Running Buffer

Dissolve 60.6 g Tris base, 97.6 g MES, 10.0 g SDS and 3.0 g EDTA in 1000 ml of ddH2O.

1X Running Buffer

Dilute 100 ml 10X running buffer with 900 ml ddH2O.

10X Transfer Buffer

Dissolve 30.0 g Tris base and 144.0 g Glycine in 1000 ml of ddH2O.

1X Transfer Buffer

Cool 1X transfer buffer to 4°C before using.

Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.

Note: For the proper high molecular weight proteins, up to 0.1% SDS may need to be added to 1X transfer buffer.

Tips for using CosmoPAGE

	1. Before sample loading, rinse the wells to remove storage buffer.
	2. Ensure tight-sealing of inner chamber when adaption (or fill outer chamber with buffer to the highest level.)
	3. Under water flow, insertgel remover between gel and cassette from well site of gel. Slowly push gel remover to the bottom of gel until gel is detached from cassette. * Avoid diagonally peeling the gel from the corner or peeling from the bottm.

Tips for Western transfer

	1. Assemble transfer sandwich by orientating cathode, sponge, 3 filter papers, gel, membrane, 3 filter papers, sponge, and anode. (The protein goes to the direction of cathode to anode.)
	2. Remove air bubbles or excess buffer between gel and membrane by carefully moving roller over the membrane.
	3. Maintain whole transfer process at low temperature. (Keep transfer tank in cold toom or on ice.)

Instruction

 CosmoPAGE Bis-tris Precast Gel 15-wells (PDF)

 CosmoPAGE Tris-glycine Precast Gel, 15-wells (PDF)

Brochure

 SDS-PAGE / Western Blotting (Brochure)

Varki Lab @UC San Diego: Hane M et al. Human-specific microglial Siglec-11 transcript variant has the potential to affect polysialic acid-mediated brain functions at a distance. Glycobiology, 2020.

Iwasaki Lab @Osaka University: Hashizume M et al. Population-Specific ACE2 Single-Nucleotide Polymorphisms Have Limited Impact on SARS-CoV-2 Infectivity In Vitro. Viruses, 2021.

Kamata Lab @University of Alabama: Skewed fate and hematopoiesis of CD34+ HSPCs in umbilical cord blood amid the COVID-19 pandemic. iScience, 2022. << NEW!