Reversed Phase / For protein and peptide separation
| Protein-R | |
|---|---|
| USP code | L1 |
| Silica gel | High purity porous spherical silica |
| Average particle size | 5 µm |
| Average pore size | approx. 300Å |
| Specific surface area | approx. 150m2/g |
| Stationary phase | Octadecyl group |
| Bonding Type | Polymeric |
| End capping treatment | Near-perfect treatment |
| pH range | 1.5 - 7.5 |
Excellent Separation
| Column size: | 4.6 mm I.D. x 150 mm | Sample: | 1. Ribonuclease A (1.0 µg) | |
| Mobile phase: | A: 0.05% TFA - 20% Acetonitrile | 2. Cytochrome C (1.0 µg) | ||
| B: 0.05% TFA - 60% Acetonitrile | 3. Lysozyme (1.0 µg) | |||
| B: 0 → 100% 20 min linear gradient | 4. BSA (1.0 µg) | |||
| Flow rate: | 1.0 ml/min | 5. Myoglobin (1.0 µg) | ||
| Temperature: | 30°C | 6. Ovalbumin (1.0 µg) | ||
| Detection: | UV 220 nm |
High Recovery Rate
Protein-R shows a higher recovery rate than conventional C18-300Å and C4-300Å columns.
Outstanding Stability at Low pH
Protein-R provides more consistent retention time and longer column lifetimes than conventional C4-300Å
For low-MW peptides, 5C18-AR-II (pore size: 120 Å) performs better; however, for high-MW peptides, Protein-R yields better separation.
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Condition Column Size: 4.6 mmI.D. x 150 mm Mobile Phase: A: 0.05% TFA-H2O / B: 0.05% TFA-MeCN, B conc. 0→35%, 60 min linear gradient Flow Rate: 1.0 ml/min Temperature: 30C Detection: UV 220 nm Sample: Poly-L-Glutamic Acid Sodium Salt, MW 1500-5000 (200µg) |
| Column | 5 µm Protein-R | |||
|---|---|---|---|---|
| I.D. (mm) | 2.0 | 4.6 | 10.0 | 20.0 |
| Washing method | Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. 2. (If you used a salt/acid, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with 50-70% acetonitrile in water containing 0.1% trifluoroacetic acid. Caution: Some proteins may precipitate when concentration of organic solvent is high. |
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| Storage conditions | Short-term (up to a week): Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water. Long-term: Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water. In either case, tightly plug the column, and store in a cool and dry place. |
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| Usable pH range | 1.5-7.5 (pH 2-7.5 recommended) | |||
| Max. pressure | 20MPa | 15MPa | ||
| Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | |||
| Usable solvents | Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable. Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points. |
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COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL Protein-R Packed Column (2.0 mm I.D. x 150 mm) | 06514-71 | Waters type | 1 pkg | 519.00 | Buy |
| COSMOSIL Protein-R Packed Column (2.0 mm I.D. x 250 mm) | 09164-71 | Waters type | 1 pkg | 749.00 | Buy |
| COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 50 mm) | 06525-31 | Waters type | 1 pkg | 535.00 | Buy |
| COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 150 mm) | 06526-21 | Waters type | 1 pkg | 541.00 | Buy |
| COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 250 mm) | 06527-11 | Waters type | 1 pkg | 632.00 | Buy |
| COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 50 mm) | 13430-41 | Waters type | 1 pkg | 942.00 | Buy |
| COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 150 mm) | 06529-91 | Waters type | 1 pkg | 1,167.00 | Buy |
| COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 250 mm) | 06530-51 | Waters type | 1 pkg | 1,482.00 | Buy |
| COSMOSIL Protein-R Packed Column (20.0 mm I.D. x 150 mm) | 06531-41 | Waters type | 1 pkg | 2,515.00 | Buy |
| COSMOSIL Protein-R Packed Column (20.0 mm I.D. x 250 mm) | 06532-31 | Waters type | 1 pkg | 3,301.00 | Buy |
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL Protein-R Guard Cartridge (4.6 mm I.D. x 10 mm) | 21249-04 | Waters type | 2 pkg | 240.00 | Buy |
| COSMOSIL Protein-R Guard Column (4.6 mm I.D. x 10 mm) | 06518-31 | Waters type | 1 pkg | 247.00 | Buy |
| COSMOSIL Protein-R Guard Column (10.0 mm I.D. x 20 mm) | 06528-01 | Waters type | 1 pkg | 552.00 | Buy |
| COSMOSIL Protein-R Guard Column (20.0 mm I.D. x 20 mm) | 08692-81 | Waters type | 1 pkg | 1,167.00 | Buy |
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL Direct Cartridge Holder | 19989-71 | Waters type | 1 pkg | 270.00 | Buy |
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | 38009-79 | Waters type | 1 pkg | - | Inquire |
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | 11884-71 | Waters type | 1 pkg | 265.00 | Buy |
| COSMOSIL Column Prefilter | 39361-19 | Waters type | 1 pkg | 145.00 | Buy |
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | 39539-09 | Waters type | 2 pkg | 55.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | 37843-69 | Waters type | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | 12570-41 | Waters type | 1 pkg | 65.00 | Buy |