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COSMOSIL Protein-R

Reversed Phase / For protein and peptide separation

  • Excellent separation
  • High recovery rate
  • Outstanding stability at low pH
 Protein-R
USP code L1
Silica gel High purity porous spherical silica
Average particle size 5 µm
Average pore size approx. 300Å
Specific surface area approx. 150m2/g
Stationary phase Octadecyl group
Bonding Type Polymeric
End capping treatment Near-perfect treatment
pH range 1.5 - 7.5

Comparison of Separation

Excellent Separation
COSMOSIL Protein-R, C18-300Å (Competitor A), C4-300Å (Competitor B)

Column size: 4.6 mm I.D. x 150 mm   Sample: 1. Ribonuclease A (1.0 µg)
Mobile phase: A: 0.05% TFA - 20% Acetonitrile     2. Cytochrome C (1.0 µg)
  B: 0.05% TFA - 60% Acetonitrile     3. Lysozyme (1.0 µg)
  B: 0 → 100% 20 min linear gradient     4. BSA (1.0 µg)
Flow rate: 1.0 ml/min     5. Myoglobin (1.0 µg)
Temperature: 30°C     6. Ovalbumin (1.0 µg)
Detection: UV 220 nm      

High Recovery Rate
Protein-R shows a higher recovery rate than conventional C18-300Å and C4-300Å columns.

Protein-R, C18-300Å (Competitor A), C4-300Å(Competitor B)

Outstanding Stability at Low pH

Protein-R provides more consistent retention time and longer column lifetimes than conventional C4-300Å

Degradation test with 0.1 % TFA solution at 60°C

Peptide Separation

For low-MW peptides, 5C18-AR-II (pore size: 120 Å) performs better; however, for high-MW peptides, Protein-R yields better separation.

Condition

Column Size: 4.6 mmI.D. x 150 mm

Mobile Phase: A: 0.05% TFA-H2O / B: 0.05% TFA-MeCN, B conc. 0→35%, 60 min linear gradient

Flow Rate: 1.0 ml/min

Temperature: 30C

Detection: UV 220 nm

Sample: Poly-L-Glutamic Acid Sodium Salt, MW 1500-5000 (200µg)

 

 

Usage Information

Column5 µm Protein-R
I.D. (mm)2.04.610.020.0
Washing method Washing method
1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid.
2. (If you used a salt/acid, always do step 1 first!) Remove adsorbed compounds and fix unstable baselines by washing with 50-70% acetonitrile in water containing 0.1% trifluoroacetic acid.
Caution: Some proteins may precipitate when concentration of organic solvent is high.
Storage conditions Short-term (up to a week):
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 methanol/20 mmol/l phosphate buffer, wash with 50:50 methanol/water.

Long-term:
Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 70% methanol or acetonitrile : 30% water.

In either case, tightly plug the column, and store in a cool and dry place.
Usable pH range 1.5-7.5 (pH 2-7.5 recommended)
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Any solvent that will not dissolve the silica gel (such as alkaline solutions) or cleave the stationary phase (such as very acidic solutions) is usable.
Note: For solvents with high viscosity, please watch the system pressure and keep it below 20 MPa. Also, please wash the column after using acidic mobile phases or solvents with high freezing points.

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Protein-R Packed Column (2.0 mm I.D. x 150 mm) 06514-71 Waters type 1 pkg 485.00 Buy
COSMOSIL Protein-R Packed Column (2.0 mm I.D. x 250 mm) 09164-71 Waters type 1 pkg 700.00 Buy
COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 50 mm) 06525-31 Waters type 1 pkg 500.00 Buy
COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 150 mm) 06526-21 Waters type 1 pkg 505.00 Buy
COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 250 mm) 06527-11 Waters type 1 pkg 590.00 Buy
COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 50 mm) 13430-41 Waters type 1 pkg 880.00 Buy
COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 150 mm) 06529-91 Waters type 1 pkg 1,090.00 Buy
COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 250 mm) 06530-51 Waters type 1 pkg 1,385.00 Buy
COSMOSIL Protein-R Packed Column (20.0 mm I.D. x 150 mm) 06531-41 Waters type 1 pkg 2,350.00 Buy
COSMOSIL Protein-R Packed Column (20.0 mm I.D. x 250 mm) 06532-31 Waters type 1 pkg 3,075.00 Buy
Guard Column
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Protein-R Guard Column (4.6 mm I.D. x 10 mm) 06518-31 Waters type 1 pkg 230.00 Buy
COSMOSIL Protein-R Guard Column (10.0 mm I.D. x 20 mm) 06528-01 Waters type 1 pkg 515.00 Buy
COSMOSIL Protein-R Guard Column (20.0 mm I.D. x 20 mm) 08692-81 Waters type 1 pkg 1,090.00 Buy