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COSMOSIL Protein-R

Reversed Phase / For protein and peptide separation

  • Excellent separation
  • High recovery rate
  • Outstanding stability at low pH
 Protein-R
USP code L1
Silica gel High purity porous spherical silica
Average particle size 5 µm
Average pore size approx. 300Å
Specific surface area approx. 150m2/g
Stationary phase Octadecyl group
Bonding Type Polymeric
End capping treatment Near-perfect treatment
pH range 1.5 - 7.5

Comparison of Separation

Excellent Separation
COSMOSIL Protein-R, C18-300Å (Competitor A), C4-300Å (Competitor B)

Column size: 4.6 mm I.D. x 150 mm   Sample: 1. Ribonuclease A (1.0 µg)
Mobile phase: A: 0.05% TFA - 20% Acetonitrile     2. Cytochrome C (1.0 µg)
  B: 0.05% TFA - 60% Acetonitrile     3. Lysozyme (1.0 µg)
  B: 0 → 100% 20 min linear gradient     4. BSA (1.0 µg)
Flow rate: 1.0 ml/min     5. Myoglobin (1.0 µg)
Temperature: 30°C     6. Ovalbumin (1.0 µg)
Detection: UV 220 nm      

High Recovery Rate
Protein-R shows a higher recovery rate than conventional C18-300Å and C4-300Å columns.

Protein-R, C18-300Å (Competitor A), C4-300Å(Competitor B)

Outstanding Stability at Low pH

Protein-R provides more consistent retention time and longer column lifetimes than conventional C4-300Å

Degradation test with 0.1 % TFA solution at 60°C

Peptide Separation

For low-MW peptides, 5C18-AR-II (pore size: 120 Å) performs better; however, for high-MW peptides, Protein-R yields better separation.

Condition

Column Size: 4.6 mmI.D. x 150 mm

Mobile Phase: A: 0.05% TFA-H2O / B: 0.05% TFA-MeCN, B conc. 0→35%, 60 min linear gradient

Flow Rate: 1.0 ml/min

Temperature: 30C

Detection: UV 220 nm

Sample: Poly-L-Glutamic Acid Sodium Salt, MW 1500-5000 (200µg)

 

 

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Protein-R Packed Column (2.0 mm I.D. x 150 mm) 06514-71 Waters type 1 pkg 485.00 Buy
COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 50 mm) 06525-31 Waters type 1 pkg 410.00 Buy
COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 150 mm) 06526-21 Waters type 1 pkg 505.00 Buy
COSMOSIL Protein-R Packed Column (4.6 mm I.D. x 250 mm) 06527-11 Waters type 1 pkg 590.00 Buy
COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 150 mm) 06529-91 Waters type 1 pkg 1,090.00 Buy
COSMOSIL Protein-R Packed Column (10.0 mm I.D. x 250 mm) 06530-51 Waters type 1 pkg 1,385.00 Buy
COSMOSIL Protein-R Packed Column (20.0 mm I.D. x 150 mm) 06531-41 Waters type 1 pkg 2,350.00 Buy
COSMOSIL Protein-R Packed Column (20.0 mm I.D. x 250 mm) 06532-31 Waters type 1 pkg 3,075.00 Buy
Guard Column
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Protein-R Guard Column (4.6 mm I.D. x 10 mm) 06518-31 Waters type 1 pkg 230.00 Buy
COSMOSIL Protein-R Guard Column (10.0 mm I.D. x 20 mm) 06528-01 Waters type 1 pkg 515.00 Buy
COSMOSIL Protein-R Guard Column (20.0 mm I.D. x 20 mm) 08692-81 Waters type 1 pkg 1,090.00 Buy