Nacalai USA - Innovations for Life Sciences

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Recombinant Mouse LIF

Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Recombinant Mouse Leukemia inhibitory factor (mLIF) is produced in E. coli. It contains a single non-glycosylated polypeptide chain 181 amino acids and has a molecular mass of 20kDa. mLIF is purified by proprietary techniques using HPLC and FPLC chromatography.

 

Source : Escherichia Coli.
Purity :
Greater than 99%, as determined by SDS-PAGE (See Fig. 1)
 
SDS-PAGE analysis of Recombinant mouse LIF Sample:
 
Endotoxin : Less than 0.01 ng/µg cytokine as determined by the LAL assay
Bioactivity :  Recombinant mouse LIF has been assessed for maintaining mouse embryonic stem cells in their undifferentiated state. Specific activity of the sample is approximate 108 units/mg. 107 units, identical 100 µg of pure protein, are sufficient to treat 10.0 L of ES cell.
Formulation : 0.22 µm filtered sterile liquid, phosphate-buffered saline with 0.02% Tween-20 and 1% BSA.
Storage :  Maintain at 4°C for 18 months. Freeze-thaw should be avoided as it results in loss of activity.

Application : Cell culture of mouse ES (CGR8)

Recombinant Mouse LIF shows the same colony forming compared to A company's product.

Application : Detection of undifferentiated markers (Nanog)

Nanog is detected in most of cells.

Data courtesy of Teruhisa Kawamura, MD, PhD, Career-Path Prootion Unit For Young Life Scientists, Kyoto University

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Recombinant Mouse LIF NU0012-1 4°C 1 ml, 106 units/ml 210.00 Buy
Recombinant Mouse LIF NU0012-2 4°C 1 ml, 107 units/ml 875.00 Buy