Recombinant Mouse LIF
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Recombinant Mouse Leukemia inhibitory factor (mLIF) is produced in E. coli. It contains a single non-glycosylated polypeptide chain 181 amino acids and has a molecular mass of 20kDa. mLIF is purified by proprietary techniques using HPLC and FPLC chromatography.
Application : Cell culture of mouse ES (CGR8)
Recombinant Mouse LIF shows the same colony forming compared to A company's product.
Application : Detection of undifferentiated markers (Nanog)
Nanog is detected in most of cells.
SDS-PAGE of mouse LIF
Source
Escherichia Coli.
Purity
Greater than 99%, as determined by SDS-PAGE (See Fig. 1)
Endotoxin
Less than 0.01 ng/µg cytokine as determined by the LAL assay
Bioactivity
Recombinant mouse LIF has been assessed for maintaining mouse embryonic stem cells in their undifferentiated state. Specific activity of the sample is approximate 108 units/mg. 107 units, identical 100 µg of pure protein, are sufficient to treat 10.0 L of ES cell.
Formulation
0.22 µm filtered sterile liquid, phosphate-buffered saline with 0.02% Tween-20 and 1% BSA.
Storage
Maintain at 4°C for 18 months. Freeze-thaw should be avoided as it results in loss of activity.