|Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of pluripotent embryonic stem cells by suppressing spontaneous differentiation. Recombinant Mouse Leukemia inhibitory factor (mLIF) is produced in E. coli. It contains a single non-glycosylated polypeptide chain 181 amino acids and has a molecular mass of 20kDa. mLIF is purified by proprietary techniques using HPLC and FPLC chromatography.|
|Source :||Escherichia Coli.|
Greater than 99%, as determined by SDS-PAGE (See Fig. 1)
SDS-PAGE analysis of Recombinant mouse LIF Sample:
|Endotoxin :||Less than 0.01 ng/µg cytokine as determined by the LAL assay|
|Bioactivity :||Recombinant mouse LIF has been assessed for maintaining mouse embryonic stem cells in their undifferentiated state. Specific activity of the sample is approximate 108 units/mg. 107 units, identical 100 µg of pure protein, are sufficient to treat 10.0 L of ES cell.|
|Formulation :||0.22 µm filtered sterile liquid, phosphate-buffered saline with 0.02% Tween-20 and 1% BSA.|
|Storage :||Maintain at 4°C for 18 months. Freeze-thaw should be avoided as it results in loss of activity.|
Recombinant Mouse LIF shows the same colony forming compared to A company's product.
Nanog is detected in most of cells.
Data courtesy of Teruhisa Kawamura, MD, PhD, Career-Path Prootion Unit For Young Life Scientists, Kyoto University