StemFit Basic04 Complete Type by Ajinomoto: iPS/ES Cell Culture Media
High-Performance iPS / ES Cell Culture Media for Basic and Clinical Research
StemFit™ Basic04 Complete Type (Basic04CT) is an animal-origin free, defined medium for human pluripotent stem cell culture. This medium includes Basic Fibroblast Growth Factor (bFGF) can be used for maintaining human ES and iPS cells under feeder-free culture conditions. It is for both research and further manufacturing use.
Features
- Animal-Origin Free
- One Bottle Composition - Basic04 Complete Type culture media contain 80 ng/ml of basic fibroblast growth factor (bFGF)
- Weekend-Free Feeding
- Single-Cell Expansion
** As of October 2024, all orders for StemFit Basic04 Complyte Type (Cat. No.: BASIC04CT) will be fulfilled using the GMP-compliant product StemFit BASIC04 Complyte Type (GMP) (Cat. No.: SF041-001). We are fully transitioning to the new GMP product, however the formulation remains unchanged, ensuring a seamless transition.
Media preparation
StemFit Basic04 Complete Type is provided frozen medium and can be stored at below -20 °C until use. Use sterile techniques when using this medium.
- Before use, thaw the frozen Basic04CT with occasionally mixing at room temperature (15-25 °C). Do not thaw, StemFit Basic04 Complete Type at 37°C, as it accelerates the degradation of medium ingredients.
If precipitations are observed, keep the bottle at room temperature and dissolve them. - Upon thawing, StemFit Basic04 Complete Type may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit Basic04 Complete Type may be stored at 2-8 °C for up to two weeks.
We recommend storing the medium protected from light. - Before use, warm aliquots at room temperature and use immediately.
Easy transition from feeder-dependent to feeder-free culture
Feeder-dependent 201B7 iPSCs were transitioned to feeder-free conditions using respective cell adhesion substrates and seeded in StemFit®.
Superior performance
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Superior growth performance on any matrices
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Superior colony-forming efficiency from a single cell clone
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Single-cell expansion
Experience superior expansion with single-cell passaging and consistent performance with various matrices.
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Top class cost-effectiveness
Weekend-free Feeding Schedules
Skip feeding on weekends for flexible scheduling
Precaution and disclaimer
StemFit Basic03 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food.
StemFit Basic03 GMP is for research and further manufacturing use only. It is not for diagnostic use, therapeutic use, or as a food.
StemFit Basic04 Complete Type is for research use only and is not intended for diagnostic or therapeutic use.
Instruction videos
Culture media preparation
Passage protocol
Referred protocols
- Technical information from the manufacturer: Movies, Technical tips, Protocols, Publications
- The latest protocol: StemFit with iMatrix-511
- Protocol for general version
- CiRA HP: http://www.cira.kyoto-u.ac.jp/e/research/protocol.html
- Nature Protocols: https://www.ncbi.nlm.nih.gov/pubmed/28005067
Publication
<Basic02>
Nakauchi Lab @Stanford University: Nishimura T. et al. Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells. Gene Ther. 2020.
<Basic03>
Kaneko Lab @CiRA, Kyoto University: Iriguchi S et al. A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy. Nat Commun, 2021.
Wernig & Oro Labs @Stanford University: Neumayer G. et al. A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa. bioRxiv, 2023. « « NEW!
Downloads
Prodcut Information - StemFit Basic04 Complete Type Ordering Information
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| StemFit™ Basic04 Complete Type (GMP) Ready-to-use, with bFGF added |
-20°C | SF041-001 | 500 mL | 485.00 | Buy |
| StemFit™ Basic04 Complete Type (GMP) Ready-to-use, with bFGF added |
-20°C | SF041-001-6 | 6 x 500 mL | - | Inquire |
Basic04
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| StemFit Basic04 Complete Type Ready-to-use, with bFGF added *** Limited period offer till stocks last. *** |
-20°C | BASIC04CT | 500 mL | - | Inquire |
- StemFit Basic04 Complete Type (#BASIC04CT) will be discontinued once the current stock is depleted. Its GMP-compliant replacement will be Basic04 Complete Type (GMP) (#SF041-001).
- As of October 2024, all orders for StemFit Basic04 Complyte Type (Cat. No.: BASIC04CT) will be fulfilled using the GMP-compliant product StemFit BASIC04 Complyte Type (GMP) (Cat. No.: SF041-001). We are fully transitioning to the new GMP product, however the formulation remains unchanged, ensuring a seamless transition.