Anti-HA (Mouse IgG1a-k), Monoclonal (HA124)
| Clone | : | HA124 |
| Isotype | : | IgG1a-κ (Mouse) |
| Product form | : | Liquid |
| Immunogen | : | Glutathione-S-Transferase (GST)-HA [YPYDVPDYA-COOH] fusion protein |
| Application | : | Western Blotting 1:1000-1:2000 ELISA 1:2000-1:20000 |
Western Blotting
| Sample | : | 1. E.coli lysate 2. NH2-(GST)(T7)(HSV)(c-Myc)(VSV)(Glu-Glu)(V5)(E-tag) (DDDDK)(S-tag)(HA)(6xHis)-COOH, (abcam, #ab5395) 250 ng 3. NH2-(GST)(HA)-COOH (Pierce, #23613) 20 ng 4. NH2-(GST)(HA)-COOH (Pierce, #23613) 100 ng 5. M.W. marker |
| Primary antibody | : | Anti-HA (Mouse IgG1-κ), Monoclonal (HA124), 1:10000, 60 min |
| Secondary antibody | : | Anti-Mouse IgG (Rat), Monoclonal Cocktail, CC, POD Conjugated, 1:25000, 60 min |
| Detection | : | Chemi-Lumi One (Cat. No.: 05027-20), 1 min |
Immunoprecipitation
| Sample | : | 1. Clarified bacterial lysate + HA-tagged GST 1µg + Anti-HA (Mouse IgG1-κ), Monoclonal (HA124), 5 µg + Protein G Agarose (50% Suspention) 0.05 ml 2. E. coli lysate + NH2-(GST)(HA)-COOH (Pierce, #23613) 1 µg |
| Detection | : | Silver staining |
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Ordering Information
| Product | Storage | Cat.No. | PKG Size | Price | |
|---|---|---|---|---|---|
| Anti-HA (Mouse IgG1-k), Monoclonal (HA124) | 2-8 °C | 06340-54 | 200 µg | 273.00 | Buy |