Detection enhancement of Rac1 with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Primary Ab: Anti-Rac1 (1: 1,000)
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500)
Sample: Rat cortical primary neuron
Detection: ECL

Data courtesy of Dr. K. Ishizuka,
Department of Psychiatry,Johns Hopkins University School of Medicine

Detection enhancement of pJNK with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T.

Primary Ab: Anti-pJNK (1: 1,000)
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
Sample: Mouse Embryonic Fibroblast (MEF)
Detection: SuperSignal West Pico

Data Courtesy of Dr. S. Matsuzawa,
Signal Transduction, NCI Cancer Center, Burnham Institute for Medical Research

 Comparison data of HIKARI with tTBS & other companies

Antibody diluent	HIKARI	tTBS	A compan      (Protein-free)	B company
Western blotting
Primary antibody	60 Minutes
	EGF Receptor (D38B1)XP Rabbit mAb(CST #4267), x 1,000 dillution XP is a trademark by Cell Signaling Technology, Inc.,
Secondary antibody	60 Minutes
	Goat anti-Rabbit IgG (H+l) Secondary Antibody [HRP] (Pre-adsorbed)(Novus #NB7187), x 50,000 dilution

 

♦ Experimental details

Sample	Hela cell extract, (left) 10μg and (right) 2 μg
Blocking	Incubated for 5 minutes with Bullet Blocking One
Detection	Chemi-Lumi One Super manufactured by Nacalai, #02230; 3-minute exposure time
Detector	LAS-300 High mode

 Signal Enhancer HIKARI (Brochure)

 Signal Enhancer HIKARI (Instruction)

 Signal Enhancer HIKARI (SDS)

1. Povero, D., et al. "HILPDA promotes NASH-driven HCC development by restraining intracellular fatty acid flux in hypoxia". J Hepatol. (2023). <<NEW!
2. Nakayasu, E. S., Syed, F., Tersey, S. A., Gritsenko, M. A., Mitchell, H. D., Chan, C. Y., ... & Metz, T. O. (2020). Comprehensive proteomics analysis of stressed human islets identifies GDF15 as a target for type 1 diabetes intervention. Cell metabolism, 31(2), 363-374.
3. Yoshie, Umehara, et al. "Robust induction of neural crest cells to derive peripheral sensory neurons from human induced pluripotent stem cells." Scientific Reports (Nature Publisher Group) 10.1 (2020).
4. Ip, Yuen K., et al. "The fluted giant clam (Tridacna squamosa) increases nitrate absorption and upregulates the expression of a homolog of SIALIN (H+: 2NO 3− cotransporter) in the ctenidium during light exposure." Coral Reefs (2020): 1-15.
5. Murata, Maho, et al. "OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma." Journal of clinical medicine 9.3 (2020): 618.
6. Hiromichi, Wakui, et al. "Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice." Scientific Reports (Nature Publisher Group) 10.1 (2020).
7. Yazawa, Keiichi, et al. "Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene–Deficient Mouse Model of Pancreatic Cancer." Translational Oncology 13.3 (2020): 100746.
8. Chan, Christabel YL, et al. "Light-enhanced phosphate absorption in the fluted giant clam, Tridacna squamosa, entails an increase in the expression of sodium-dependent phosphate transporter 2a in its colourful outer mantle." Coral Reefs: 1-16.
9. Taniguchi, Keiko, et al. "Depletion of gamma-glutamylcyclotransferase inhibits cancer cell growth by activating the AMPK–FOXO3a–p21 axis." Biochemical and biophysical research communications 517.2 (2019): 238-243.
10. Kamemura, Norio, et al. "Cross-allergenicity of crustacean and the edible insect Gryllus bimaculatus in patients with shrimp allergy." Molecular immunology 106 (2019): 127-134.
11. Ip, Yuen K., et al. "The fluted giant clam (Tridacna squamosa) increases nitrate absorption and upregulates the expression of a homolog of SIALIN (H+: 2NO 3− cotransporter) in the ctenidium during light exposure." Coral Reefs (2020): 1-15.
12. Aziati, Ishmael Dzigbordi, et al. "PATZ1 is required for efficient HIV-1 infection." Biochemical and biophysical research communications 514.2 (2019): 538-544.
13. Cao-Pham, Anh H., et al. "Calcium absorption in the fluted giant clam, Tridacna squamosa, may involve a homolog of voltage-gated calcium channel subunit α1 (CACNA1) that has an apical localization and displays light-enhanced protein expression in the ctenidium." Journal of Comparative Physiology B 189.6 (2019): 693-706.