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Signal Enhancer HIKARI

Signal Enhancer for Western Blotting and ELISA

Dilute your antibodies with Signal Enhancer HIKARI instead of conventional diluents such as PBS or TBS before performing your next Western blot detection protocol and witness a remarkable increase in the ability to detect the protein of interest and to eliminate undesired background.
Signal Enhancer HIKARI was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA.

Features

  • Enhances antigen-antibody reactions - yields several 10-fold increase in signal intensity
  • Removes background - improves the specificity of your antibodies
  • Works with any substrate - enhances both chemiluminescence and colorimetric detection
  • Works with any membrane - enhances signal from nitrocellulose and PVDF membrane
  • Ready-to-use reagent - just dilutes your antibodies with Signal Enhancer HIKARI

Application 1

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Primary Ab: Anti-Rac1 (1: 1,000)
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500)
Sample: Rat cortical primary neuron
Detection: ECL

Data courtesy of Dr. K. Ishizuka,
Department of Psychiatry,Johns Hopkins University School of Medicine

Application 2

Detection enhancement of pJNK with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T.

Primary Ab: Anti-pJNK (1: 1,000)
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
Sample: Mouse Embryonic Fibroblast (MEF)
Detection: SuperSignal West Pico

Data Courtesy of Dr. S. Matsuzawa,
Signal Transduction, NCI Cancer Center, Burnham Institute for Medical Research

Technical Information

Signal Enhancer HIKARI Procedure (PDF)

MSDS

Signal Enhancer HIKARI (PDF)

Reference

  1. Yoshie, Umehara, et al. "Robust induction of neural crest cells to derive peripheral sensory neurons from human induced pluripotent stem cells." Scientific Reports (Nature Publisher Group) 10.1 (2020).
  2. Ip, Yuen K., et al. "The fluted giant clam (Tridacna squamosa) increases nitrate absorption and upregulates the expression of a homolog of SIALIN (H+: 2NO 3− cotransporter) in the ctenidium during light exposure." Coral Reefs (2020): 1-15.
  3. Murata, Maho, et al. "OVOL2-Mediated ZEB1 Downregulation May Prevent Promotion of Actinic Keratosis to Cutaneous Squamous Cell Carcinoma." Journal of clinical medicine 9.3 (2020): 618.
  4. Hiromichi, Wakui, et al. "Effects of Rikkunshito treatment on renal fibrosis/inflammation and body weight reduction in a unilateral ureteral obstruction model in mice." Scientific Reports (Nature Publisher Group) 10.1 (2020).
  5. Yazawa, Keiichi, et al. "Low Incidence of High-Grade Pancreatic Intraepithelial Neoplasia Lesions in a Crmp4 Gene–Deficient Mouse Model of Pancreatic Cancer." Translational Oncology 13.3 (2020): 100746.
  6. Chan, Christabel YL, et al. "Light-enhanced phosphate absorption in the fluted giant clam, Tridacna squamosa, entails an increase in the expression of sodium-dependent phosphate transporter 2a in its colourful outer mantle." Coral Reefs: 1-16.
  7. Taniguchi, Keiko, et al. "Depletion of gamma-glutamylcyclotransferase inhibits cancer cell growth by activating the AMPK–FOXO3a–p21 axis." Biochemical and biophysical research communications 517.2 (2019): 238-243.
  8. Kamemura, Norio, et al. "Cross-allergenicity of crustacean and the edible insect Gryllus bimaculatus in patients with shrimp allergy." Molecular immunology 106 (2019): 127-134.
  9. Ip, Yuen K., et al. "The fluted giant clam (Tridacna squamosa) increases nitrate absorption and upregulates the expression of a homolog of SIALIN (H+: 2NO 3− cotransporter) in the ctenidium during light exposure." Coral Reefs (2020): 1-15.
  10. Aziati, Ishmael Dzigbordi, et al. "PATZ1 is required for efficient HIV-1 infection." Biochemical and biophysical research communications 514.2 (2019): 538-544.
  11. Cao-Pham, Anh H., et al. "Calcium absorption in the fluted giant clam, Tridacna squamosa, may involve a homolog of voltage-gated calcium channel subunit α1 (CACNA1) that has an apical localization and displays light-enhanced protein expression in the ctenidium." Journal of Comparative Physiology B 189.6 (2019): 693-706.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Signal Enhancer HIKARI 50 (Solution 1* 50 ml, Solution 2** 50 ml) NU00101 4°C 1 Kit 98.00 Buy
Signal Enhancer HIKARI 250 (Solution 1* 250 ml, Solution 2** 250 ml) NU00102 4°C 1 Kit 298.00 Buy
Signal Enhancer HIKARI - Solution 1 NU00102-1 4°C 250 ml 170.00 Buy
Signal Enhancer HIKARI - Solution 2 NU00102-2 4°C 250 ml 170.00 Buy

* Solution 1 for Primary Antibody
** Solution 2 for Secondary Antibody