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COSMOSIL 3C18-EB

Reversed Phase / Monomeric C18

  • Excellent for Basic compounds
  • Improved separation for acidic or metal coordination compounds
  • Simple mobile phase conditions
COSMOSIL 3C18-EB
USP code L1
Silica gel High purity porous spherical silica
Average particle size 3 µm
Average pore size approx. 120Å
Specific surface area approx. 300m2/g
Stationary phase Octadecyl group
Bonding type Monomeric type
End capping treatment Near-perfect treatment
Carbon content approx. 14.5%
pH range 2 - 10

Analysis of Basic Compounds

Conventionally end-capped C18 columns still have many residual silanol groups on the silica surface of stationary phase that can form ionic bonds with basic compounds. The resulting peak tailing makes accurate quantification of analytes difficult, especially in trace analyses. COSMOSIL 3C18-EB with a better end-capping treatment offers improved peak shape and separation particularly for basic compounds.

Comparison 1 (Basic Compounds)

Column size: 4.6 mm I.D. x 75 mm Sample: 1. Amitriptyline 0.2mg/ml
2. Propylbenzene (I.S.) 2.0mg/ml
Mobile phase: Acetonitrile/ 20mmol/l Phosphate Buffer(pH7.0) = 60/40
Flow rate: 1.0 ml/min Inj. Vol: 2.0 µl
Temperature: 40°C    
Detection: UV 254 nm    
 

Comparison 2 (Basic Compounds)

Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH7.0) = 60/40
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 235 nm
Sample: 1. Dextromethorphan 0.5mg/ml
2. Benzene (I.S.) 7.5mg/ml
Inj. Vol: 1.5 µl

 

Comparison 3 (Characteristic)

 

Analysis of Acidic or Metal Coordination Compounds

Acidic or metal coordination compounds can also form bonds with residual silanol groups and metal impurities causing peak tailing. COSMOSIL 3C18-EB with a better end-capping treatment and high purity silica gel offers improved peak shape and separation for acidic or metal coordination compounds.

Acidic Compounds

COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH2.5) = 30/70
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Salicylic Acid 1.0 mg/ml
2. Ethyl p-Hydroxybenzoate (I.S.) 0.05 mg/ml

Metal Coordination Compounds

COSMOSIL 3C18-EB
Column size: 4.6 mm I.D. x 75 mm
Mobile phase: Acetonitrile/ 20mmol/l
Phosphoric Acid = 10/90
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Oxine-copper 0.03 mg/ml
2. Caffeine (I.S.) 0.3 mg/ml

Inj. Vol: 2.0 µl

Synthesis Reproducibility

If you use insufficiently end-capped C18 columns for basic compound analyses, you may need to spend much time adjusting mobile phase conditions. You may need
  1. more than 3 or more organic solvents or buffers
  2. ion-pair reagents or additives
  3. buffers in different pH

In addition, the complexity of the mobile phase often is detrimental to reproducibility. COSMOSIL 3C18-EB with a new end-capping treatment allows good peak shape and separation using simple mobile phase conditions.

Analysis with Simple Condition

Complicated Condition Simple Condition
Methanol/ Acetonitrile/ 20mmol/l KH2PO4, 0.2%Triethylamine(pH3.0 with H3PO4) = 35/15/50(*) Acetonitrile/ 20mmol/l Phosphate Buffer(pH2.5) = 30/70
(*): USP 38(1) Amlodipine and Benazepril Hydrochloride Capsules, Performance Tests  
Column size: 4.6 mm I.D. x 100 mm
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 237 nm
Sample: 1. Amlodipine 0.0077mg/ml
2. Benazepril 0.0225mg/ml
Inj. Vol: 50 µl

Routine Analysis

3C18-EB has excellent synthesis reproducibility and column-to-column reproducibility, so it is applicable in routine analysis such as quality control of drugs.

Excellent Synthesis Reproducibility

Column size: 4.6 mm I.D. x 150 mm Column size: 4.6 mm I.D. x 150 mm
Mobile phase: Acetonitrile / H2O = 60/40 Mobile phase: Acetonitrile/ 20mmol/l Phosphate
Buffer(pH7.0) = 60/40
Flow rate: 1.0 ml/min Flow rate: 1.0 ml/min
Temperature: 40°C Temperature: 40°C
Detection: UV 254 nm Detection: UV 254 nm
Sample: 1. Acetophenone 0.05 mg/ml
2. Methyl Benzoate 0.5 mg/ml
3. Benzene 2.0 mg/ml
4. Toluene 2.0 mg/ml
Sample: 1. Amitriptyline 0.2 mg/ml
2. Propylbenzene (I.S.) 2.0 mg/ml
Inj. Vol.:  1.5 µl Inj. Vol.:  2.0 µl

Analysis under Ammonium Acetate Buffer

COSMOSIL 3C18-EB produces sharp peaks using ammonium acetate buffer in LC-MS.

Excellent Synthesis Reproducibility

Acetonitrile/ 20mmol/l Phosphate buffer(pH7.0) = 60/40 Acetonitrile/ 10mmol/l CH3COONH4 = 60/40
Column size: 4.6 mm I.D. x 75 mm
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 254 nm
Sample: 1. Amitriptyline 0.2mg/ml
2. Propylbenzene (I.S.) 2.0mg/ml

Inj. Vol: 1.0 µl

Analysis of standards in USP

Excellent Synthesis Reproducibility

USP 37(3) Esomeprazole Magnesium Delayed-Release Capsules, Impurities

Column size: 4.6 mm I.D. x 100 mm
Mobile phase: A; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 10/90
B; Acetonitrile/ 36.7mmol/l Phosphate Buffer(pH7.6) = 80/20
B conc. 0→ 20%(0→ 10min), 20→ 100%(10→ 30min)
Flow rate: 1.0 ml/min
Temperature: 40°C
Detection: UV 302 nm
Sample: 1. Omeprazole related compound A 0.001mg/ml
2. Omeprazole 0.001mg/ml
Inj. Vol: 20 µl
USP 37(3) Esomeprazole Magnesium Delayed-Release Capsules, Impurities

 

 USP 28(6) Hydrochlorothiazide, Assay, Related Compounds

 

USP 38(1) Amlodipine and Benazepril Hydrochloride, Capsules, Performance Tests

Applications

Oligonucleotides

  

Biphenylcarboxylic Acid

 

Naphthoic Acids

 

Phthalic Acids

 

beta-Blockers (Metoprolol, Oxprenolol and Alprenolol)

 

Quinolone Antimicrobials (Ciprofloxacin, Danofloxacin, Enrofloxacin and Sarafloxacin)

 

Aminoglycoside Antibiotics (Spectinomycin, Hygromycin B, Streptomycin, Dihydrostreptomycin, Amikacin, Kanamycin, Apramycin, Tobramycin, Gentamicin and Neomycin)

 

Surfactants

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 3C18-EB Packed Column (1.0 mm I.D. x 50 mm) 11386-01 Waters type 1 pkg 440.00 Buy
COSMOSIL 3C18-EB Packed Column (2.0 mm I.D. x 50 mm) 09794-21 Waters type 1 pkg 420.00 Buy
COSMOSIL 3C18-EB Packed Column (2.0 mm I.D. x 75 mm) 09795-11 Waters type 1 pkg 450.00 Buy
COSMOSIL 3C18-EB Packed Column (2.0 mm I.D. x 100 mm) 09796-01 Waters type 1 pkg 475.00 Buy
COSMOSIL 3C18-EB Packed Column (2.0 mm I.D. x 150 mm) 09797-91 Waters type 1 pkg 525.00 Buy
COSMOSIL 3C18-EB Packed Column (2.0 mm I.D. x 250 mm) 09798-81 Waters type 1 pkg 680.00 Buy
COSMOSIL 3C18-EB Packed Column (3.0 mm I.D. x 50 mm) 09799-71 Waters type 1 pkg 420.00 Buy
COSMOSIL 3C18-EB Packed Column (3.0 mm I.D. x 75 mm) 09800-21 Waters type 1 pkg 450.00 Buy
COSMOSIL 3C18-EB Packed Column (3.0 mm I.D. x 100 mm) 09811-81 Waters type 1 pkg 475.00 Buy
COSMOSIL 3C18-EB Packed Column (3.0 mm I.D. x 150 mm) 09814-51 Waters type 1 pkg 525.00 Buy
COSMOSIL 3C18-EB Packed Column (3.0 mm I.D. x 250 mm) 09827-91 Waters type 1 pkg 680.00 Buy
COSMOSIL 3C18-EB Packed Column (4.6 mm I.D. x 50 mm) 09840-01 Waters type 1 pkg 420.00 Buy
COSMOSIL 3C18-EB Packed Column (4.6 mm I.D. x 75 mm) 09841-91 Waters type 1 pkg 450.00 Buy
COSMOSIL 3C18-EB Packed Column (4.6 mm I.D. x 100 mm) 09842-81 Waters type 1 pkg 475.00 Buy
COSMOSIL 3C18-EB Packed Column (4.6 mm I.D. x 150 mm) 09843-71 Waters type 1 pkg 525.00 Buy
COSMOSIL 3C18-EB Packed Column (4.6 mm I.D. x 250 mm) 09844-61 Waters type 1 pkg 680.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL 3C18-EB Guard Cartridge (2.0 mm I.D. x 10 mm) 11892-74 Waters type 2 pkg 200.00 Buy
COSMOSIL 3C18-EB Guard Cartridge (4.6 mm I.D. x 10 mm) 11890-94 Waters type 2 pkg 200.00 Buy
COSMOSIL 3C18-EB Guard Column (4.6 mm I.D. x 10 mm) 09839-41 Waters type 1 pkg 275.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Direct Cartridge Holder 19989-71 Waters type 1 pkg 270.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy

** Guard cartridges require a guard cartridge holder.