COSMOSIL NH2-MS
Saccharide Separation
The COSMOSIL 5NH2-MS is a column with aminopropyl group bonded silica packing material. It is recommended for separation of monosaccharides and oligosaccharides. While Sugar-D is our first choice for separation of saccharide, 5NH2-MS can give better separation than Sugar-D in some cases depending on samples.
| NH2-MS | |
|---|---|
| USP code | L8 |
| Silica gel | High purity porous spherical silica |
| Average particle size | 5 µm |
| Average pore size | approx. 300Å |
| Specific surface area | approx. 120m2/g |
| Stationary phase | Aminopropyl group |
| Bonding Type | Polymeric |
| End capping treatment | Near-perfect treatment |
| Carbon content | approx. 4% |
Comparison of Retention
The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10% as shown below.
| Column size: | 4.6 mm I.D. x 250 mm |
| Flow rate: | 1 ml/min |
| Temperature: | 30°C |
| Detection: | RI |
| Sample: | 1. Glucose 2. Maltose |
Usage Information
| Column | 5 µm NH2 | ||||
|---|---|---|---|---|---|
| I.D. (mm) | 2.0 | 3.0 | 4.6 | 10.0 | 20.0 |
| Washing method | 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water. 2. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water. |
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| Storage conditions | Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. Then, replace the solvent with 90:10 acetonitrile/water. Tightly plug the column, and store in a cool and dry place. |
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| Usable pH range | pH2～pH7.5 | ||||
| Max. pressure | 20MPa | 15MPa | |||
| Temperature range | The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C. | ||||
| Usable solvents | - Acetonitrile/water mobile phases are recommended. | ||||
| Other instructions | - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L. - Always filter mobile phases using a 0.5 µm or finer filter before use. - Methanol is not recommended as a mobile phase. - If using a buffer or acid, we recommend using the column only for that mobile phase. Buffers may affect performance even after washing the column. - Neutral pH will maximize retention time. |
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COSMOSIL Application Search
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
Ordering Information
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL NH2-MS Packed Column (2.0 mm I.D. x 150 mm) | 05251-41 | Waters type | 1 pkg | 514.00 | Buy |
| COSMOSIL NH2-MS Packed Column (2.0 mm I.D. x 250 mm) | 09185-01 | Waters type | 1 pkg | - | Inquire |
| COSMOSIL NH2-MS Packed Column (3.0 mm I.D. x 150 mm) | 08425-81 | Waters type | 1 pkg | 514.00 | Buy |
| COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 50 mm) | 38243-31 | Waters type | 1 pkg | 439.00 | Buy |
| COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 100 mm) | 38244-21 | Waters type | 1 pkg | 461.00 | Buy |
| COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 150 mm) | 38245-11 | Waters type | 1 pkg | 461.00 | Buy |
| COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 250 mm) | 38246-01 | Waters type | 1 pkg | 530.00 | Buy |
| COSMOSIL NH2-MS Packed Column (6.0 mm I.D. x 150 mm) | 38247-91 | Waters type | 1 pkg | 642.00 | Buy |
| COSMOSIL NH2-MS Packed Column (6.0 mm I.D. x 250 mm) | 38248-81 | Waters type | 1 pkg | 910.00 | Buy |
| COSMOSIL NH2-MS Packed Column (10.0 mm I.D. x 150 mm) | 34512-21 | Waters type | 1 pkg | 1,124.00 | Buy |
| COSMOSIL NH2-MS Packed Column (10.0 mm I.D. x 250 mm) | 38249-71 | Waters type | 1 pkg | 1,127.00 | Buy |
| COSMOSIL NH2-MS Packed Column (20.0 mm I.D. x 250 mm) | 38250-31 | Waters type | 1 pkg | 2,595.00 | Buy |
| COSMOSIL NH2-MS Packed Column (28.0 mm I.D. x 250 mm) | 08226-91 | Waters type | 1 pkg | 7,383.00 | Buy |
| COSMOSIL NH2-MS Guard Column (28.0 mm I.D. x 50 mm) | 08225-01 | Waters type | 1 pkg | 4,066.00 | Buy |
Guard Column / Guard Cartridge
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL NH2-MS Guard Cartridge (4.6 mm I.D. x 10 mm) | 19182-34 | Waters type | 2 pkg | 214.00 | Buy |
| COSMOSIL NH2-MS Guard Column (10.0 mm I.D. x 20 mm) | 38242-41 | Waters type | 1 pkg | 514.00 | Buy |
| COSMOSIL NH2-MS Guard Column (20.0 mm I.D. x 50 mm) | 06093-91 | Waters type | 1 pkg | 1,322.00 | Buy |
HPLC Accessories
| Product | Cat.No. | End-Fitting | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| COSMOSIL Direct Cartridge Holder | 19989-71 | Waters type | 1 pkg | 270.00 | Buy |
| COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** | 38009-79 | Waters type | 1 pkg | - | Inquire |
| COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) | 11884-71 | Waters type | 1 pkg | 265.00 | Buy |
| COSMOSIL Column Prefilter | 39361-19 | Waters type | 1 pkg | 145.00 | Buy |
| COSMOSIL Column Spare Filter for Prefilter (1 micron) | 39539-09 | Waters type | 2 pkg | 55.00 | Buy |
| COSMOSIL Column Connecting Tube (0.25 mm) | 37843-69 | Waters type | 1 pkg | 65.00 | Buy |
| COSMOSIL Column Connecting Tube (0.1 mm) | 12570-41 | Waters type | 1 pkg | 65.00 | Buy |
** Guard cartridges require a guard cartridge holder.
** #38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).