Nacalai USA - Innovations for Life Sciences

Subtotal: $0.00

View Cart

COSMOSIL NH2-MS

Saccharide Separation

The COSMOSIL 5NH2-MS is a column with aminopropyl group bonded silica packing material. It is recommended for separation of monosaccharides and oligosaccharides. While Sugar-D is our first choice for separation of saccharide, 5NH2-MS can give better separation than Sugar-D in some cases depending on samples.

 NH2-MS
USP code L8
Silica gel High purity porous spherical silica
Average particle size 5 µm
Average pore size approx. 300Å
Specific surface area approx. 120m2/g
Stationary phase Aminopropyl group
Bonding Type Polymeric
End capping treatment Near-perfect treatment
Carbon content approx. 4%

Comparison of Retention

The conventional aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5%-10% as shown below.

Column size: 4.6 mm I.D. x 250 mm
Flow rate: 1 ml/min
Temperature: 30°C
Detection: RI
Sample: 1. Glucose
2. Maltose
 

Usage Information

Column5 µm NH2
I.D. (mm)2.03.04.610.020.0
Washing method 1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water.

2. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water.
Storage conditions Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid.
Then, replace the solvent with 90:10 acetonitrile/water.

Tightly plug the column, and store in a cool and dry place.
Usable pH range pH2~pH7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents - Acetonitrile/water mobile phases are recommended.
Other instructions - Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
- Always filter mobile phases using a 0.5 µm or finer filter before use.
- Methanol is not recommended as a mobile phase.
- If using a buffer or acid, we recommend using the column only for that mobile phase. Buffers may affect performance even after washing the column.
- Neutral pH will maximize retention time.

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL NH2-MS Packed Column (2.0 mm I.D. x 150 mm) 05251-41 Waters type 1 pkg 480.00 Buy
COSMOSIL NH2-MS Packed Column (2.0 mm I.D. x 250 mm) 09185-01 Waters type 1 pkg N/A Inquire
COSMOSIL NH2-MS Packed Column (3.0 mm I.D. x 150 mm) 08425-81 Waters type 1 pkg 480.00 Buy
COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 50 mm) 38243-31 Waters type 1 pkg 410.00 Buy
COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 100 mm) 38244-21 Waters type 1 pkg 430.00 Buy
COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 150 mm) 38245-11 Waters type 1 pkg 430.00 Buy
COSMOSIL NH2-MS Packed Column (4.6 mm I.D. x 250 mm) 38246-01 Waters type 1 pkg 495.00 Buy
COSMOSIL NH2-MS Packed Column (6.0 mm I.D. x 150 mm) 38247-91 Waters type 1 pkg 600.00 Buy
COSMOSIL NH2-MS Packed Column (6.0 mm I.D. x 250 mm) 38248-81 Waters type 1 pkg 850.00 Buy
COSMOSIL NH2-MS Packed Column (10.0 mm I.D. x 150 mm) 34512-21 Waters type 1 pkg 1,050.00 Buy
COSMOSIL NH2-MS Packed Column (10.0 mm I.D. x 250 mm) 38249-71 Waters type 1 pkg 1,030.00 Buy
COSMOSIL NH2-MS Packed Column (20.0 mm I.D. x 250 mm) 38250-31 Waters type 1 pkg 2,425.00 Buy
COSMOSIL NH2-MS Packed Column (28.0 mm I.D. x 250 mm) 08226-91 Waters type 1 pkg 6,900.00 Buy
COSMOSIL NH2-MS Guard Column (28.0 mm I.D. x 50 mm) 08225-01 Waters type 1 pkg 3,800.00 Buy
Guard Column / Guard Cartridge
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL NH2-MS Guard Cartridge (4.6 mm I.D. x 10 mm) 19182-34 Waters type 1 pkg 200.00 Buy
COSMOSIL NH2-MS Guard Column (4.6 mm I.D. x 10 mm) 38241-51 Waters type 1 pkg 200.00 Buy
COSMOSIL NH2-MS Guard Column (10.0 mm I.D. x 20 mm) 38242-41 Waters type 1 pkg 480.00 Buy
COSMOSIL NH2-MS Guard Column (20.0 mm I.D. x 50 mm) 06093-91 Waters type 1 pkg 1,235.00 Buy
HPLC Accessories
Product Cat.No. End-Fitting PKG Size Price(US$)  
COSMOSIL Direct Cartridge Holder 19989-71 Waters type 1 pkg 270.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) 38009-79 Waters type 1 pkg 265.00 Buy
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) 11884-71 Waters type 1 pkg 265.00 Buy
COSMOSIL Column Prefilter 39361-19 Waters type 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) 39539-09 Waters type 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) 37843-69 Waters type 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) 12570-41 Waters type 1 pkg 65.00 Buy

** Guard cartridges require a guard cartridge holder.