Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded phases are tailing and adsorption of certain saccharides and general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D, resulting in better (sharper) separation and much improved durability. In addition COSMOSIL Sugar-D is useful in the separation of highly hydrophilic compounds which are not retained in conventional octadecyl (ODS) bounded stationary phases.
Certain types of saccharides such as Arabinose or Galactose are partially or temporarily absorbed in conventional Aminopropyl stationary phases causing disappearance of sample or tailing. These problematic saccharides elute sharply and in proportion of the injection volume from the superior COSMOSIL Sugar-D.
Development of Sugar-D
Aldehyde groups in the open-chain form of sugars can form Schiff bases with the amino groups on aminopropyl columns, causing low recovery and peak tailing. COSMOSIL Sugar-D was designed to avoid this, so sugars like arabinose and galactose, which are problematic on aminopropyl columns, elute with sharp peaks. Sugar-D is especially useful for samples of low concentration.
Quantitation at low concentration
The decrease of retention time was compared between COSMOSIL Sugar-D and conventional Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case of COSMOSIL Sugar-D.
The separation performances and the adsorption of monosaccharides were compared in COSMOSIL Sugar-D and other companies' columns. The presence of Aldehyde-group in the sample saccharides (except of Fructose) does not result in undesirable adsorption, therefore has no ill effect on the performance of COSMOSIL Sugar-D column.
We have compiled a list of our Sugar-D HPLC application data in an easy-to-read format. Please click the compound name to view its data.
|Cyclodextrins||10016-20-3 / 7585-39-9 / 17465-86-0|
The conventional Aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5% - 10%.
Column size 4.6mmI.D.×250mm Flow rate 1.0ml/min Temperature: 30°C Detection: RI Sample 1: Glucose 2: Maltose
Separation of Saccharides
Mono- and Oligosaccharides Polyols
Sugars in Sports Drinks (AP-0327)
Sugar Alcohols (AP-0330)
Commercially Energy Drink (AP-1049)
Acarbose Analysis (PDF file)
Acarbose is used to treat type 2 diabetes. A recent study shows efficacy and tolerability of acarbose in patients with type 2 diabetes. By using COSOMSIL Sugar-D, which is designed for saccharide analysis, highresolution separation of Acarbose and its impurities was obtained. Furthermore, COSMOSIL Sugar-D was more durable than the conventional NH2 column.
Analysis of Sugars in Honey and Syrup (PDF file)
Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability.
|Column||5 µm Sugar-D|
|Storage conditions||Wash the column to remove buffer, salts and/or acid.
Then, replace the solvent with 90:10 acetonitrile/water.
Tightly plug the column, and store in a cool and dry place.
|Usable pH range||pH 2 - 7.5|
|Temperature range||The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.|
|Usable solvents||Acetonitrile/water mobile phases are recommended.|
COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size
|COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 50 mm)||07974-21||Waters type||1 pkg||480.00||Buy|
|COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 100 mm)||15378-91||Waters type||1 pkg||N/A||Inquire|
|COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 150 mm)||05688-41||Waters type||1 pkg||510.00||Buy|
|COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 250 mm)||05689-31||Waters type||1 pkg||615.00||Buy|
|COSMOSIL Sugar-D Packed Column (3.0 mm I.D. x 150 mm)||05690-91||Waters type||1 pkg||460.00||Buy|
|COSMOSIL Sugar-D Packed Column (3.0 mm I.D. x 250 mm)||05691-81||Waters type||1 pkg||540.00||Buy|
|COSMOSIL Sugar-D Packed Column (4.6 mm I.D. x 50 mm)||07975-11||Waters type||1 pkg||400.00||Buy|
|COSMOSIL Sugar-D Packed Column (4.6 mm I.D. x 150 mm)||05395-71||Waters type||1 pkg||430.00||Buy|
|COSMOSIL Sugar-D Packed Column (4.6 mm I.D. x 250 mm)||05397-51||Waters type||1 pkg||515.00||Buy|
|COSMOSIL Sugar-D Packed Column (10.0 mm I.D. x 250 mm)||05692-71||Waters type||1 pkg||1,210.00||Buy|
|COSMOSIL Sugar-D Packed Column (20.0 mm I.D. x 250 mm)||05693-61||Waters type||1 pkg||2,540.00||Buy|
|COSMOSIL Sugar-D Guard Cartridge (4.6 mm I.D. x 10 mm)||19185-04||Waters type||2 pkg||220.00||Buy|
|COSMOSIL Sugar-D Guard Column (4.6 mm I.D. x 10 mm)||05394-81||Waters type||1 pkg||265.00||Buy|
|COSMOSIL Sugar-D Guard Column (10.0 mm I.D. x 20 mm)||05696-31||Waters type||1 pkg||590.00||Buy|
|COSMOSIL Sugar-D Guard Column (20.0 mm I.D. x 50 mm)||05694-51||Waters type||1 pkg||1,365.00||Buy|
|COSMOSIL Direct Cartridge Holder||19989-71||Waters type||1 pkg||270.00||Buy|
|COSMOSIL Guard Cartridge Holder (4.6 mm I.D.)||38009-79||Waters type||1 pkg||265.00||Buy|
|COSMOSIL Guard Cartridge Holder (2.0 mm I.D.)||11884-71||Waters type||1 pkg||265.00||Buy|
|COSMOSIL Column Prefilter||39361-19||Waters type||1 pkg||145.00||Buy|
|COSMOSIL Column Spare Filter for Prefilter (1 micron)||39539-09||Waters type||2 pkg||55.00||Buy|
|COSMOSIL Column Connecting Tube (0.25 mm)||37843-69||Waters type||1 pkg||65.00||Buy|
|COSMOSIL Column Connecting Tube (0.1 mm)||12570-41||Waters type||1 pkg||65.00||Buy|