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Saccharide Separation

Conventionally Aminopropyl bounded stationary phases are used for liquid chromatographic analysis of mono- and oligosaccharides. General shortcomings of the conventional Aminopropyl bounded phases are tailing and adsorption of certain saccharides and general low durability (short active life) of these columns. These problems are addressed and solved by the novel COSMOSIL Sugar-D, resulting in better (sharper) separation and much improved durability. In addition COSMOSIL Sugar-D is useful in the separation of highly hydrophilic compounds which are not retained in conventional octadecyl (ODS) bounded stationary phases.

Certain types of saccharides such as Arabinose or Galactose are partially or temporarily absorbed in conventional Aminopropyl stationary phases causing disappearance of sample or tailing. These problematic saccharides elute sharply and in proportion of the injection volume from the superior COSMOSIL Sugar-D.

  • Different selectivity from aminopropyl columns
  • Superior quantitative results
  • Superior durability
  • Anomers remain unseparated
USP code N/A
Silica gel High purity porous spherical silica
Average particle size 5 µm
Stationary phase Secondary / tertiary amine

About Sugar-D

Development of Sugar-D

Aminopropyl columns are commonly used for analysis of monosaccharides and oligosaccharides; however, this type of column has several problems, including low durability, poor separation, and adsorption and peak tailing with some analytes. Carbamoylbased columns separate anomers, which may not be desirable. To solve these problems, we developed a specialty column for sugar analysis with high durability and performance that does not induce irreversible adsorption.

Superior quantitative results

Aldehyde groups in the open-chain form of sugars can form Schiff bases with the amino groups on aminopropyl columns, causing low recovery and peak tailing. COSMOSIL Sugar-D was designed to avoid this, so sugars like arabinose and galactose, which are problematic on aminopropyl columns, elute with sharp peaks. Sugar-D is especially useful for samples of low concentration.

Quantitation at low concentration


Superior durability

The decrease of retention time was compared between COSMOSIL Sugar-D and conventional Aminopropyl bounded stationary phase under severe condition of 100% water as eluent between tests. The capacity factor did not decrease in case of COSMOSIL Sugar-D.

Eluent condition
Flow rate
: water
: 1.0 ml/min
: RT
Test condition
Column size
Mobile phase
Flow rate
: 4.6 mmI.D.×250 mm
: acetonitrile:water=70:30
: 1.0 ml/min
: 30°C
: RI
: Maltose


Comparison to amide column

The separation performances and the adsorption of monosaccharides were compared in COSMOSIL Sugar-D and other companies' columns. The presence of Aldehyde-group in the sample saccharides (except of Fructose) does not result in undesirable adsorption, therefore has no ill effect on the performance of COSMOSIL Sugar-D column.

Acidic Sugar

Analysis of Carbohydrate Mixtures by COSMOSIL Sugar-D

We have compiled a list of our Sugar-D HPLC application data in an easy-to-read format. Please click the compound name to view its data.

CoumpoundCAS No.
D-(+)-Glucose 50-99-7
D-(-)-Fructose 57-48-7
Xylitol 87-99-0
D-Psicose 551-68-8
Sucrose 57-50-1
Maltose 6363-53-7
D-(+)-Raffinose 17629-30-0
D-(+)-Mannose 3458-28-4
L-(+)-Rhamnose 10030-85-0
D-(+)-Trehalose 6138-23-4
D-(+)-Allose 2595-97-3
D-Glucitol 50-70-4
Maltitol 585-88-6
meso-Erythritol [meso-Erythrite] 149-32-6
myo-Inositol 87-89-8
Cyclodextrins 10016-20-3 / 7585-39-9 / 17465-86-0
D-Glucuronic Acid 6556-12-3
Glycerol 56-81-5
1-Kestose 470-69-9
Lactose 5989-81-1
Maltotriitol 32860-62-1
Maltotriose 1109-28-0
Maltotetraose 34612-38-9
Maltopentaose 1668-09-3
Maltohexaose 34620-77-4
Maltoheptaose 34620-78-5
D-(-)-Mannitol 69-65-8
D-Mannosamine 5505-63-5
Nystose 13133-07-8
Palatinit 64519-82-0
Palatinose 13718-94-0
D-(+)-Xylose 58-86-6


Retention time
The conventional Aminopropyl column is slightly more retentive than Sugar-D. The retention time can be adjusted by increasing the concentration of acetonitrile in the mobile phase by 5% - 10%.

Column size 4.6mmI.D.×250mm Flow rate 1.0ml/min Temperature: 30°C Detection: RI Sample 1: Glucose 2: Maltose


Separation of Saccharides
Mono- and Oligosaccharides Polyols

Amino Sugar


Sugars in Sports Drinks (AP-0327)


Honey (AP-1530) (AP-1532) (AP-1533)



Sugar Alcohols (AP-0330)


Maltooligosaccharides (AP-0331)


Commercially Energy Drink (AP-1049)



Acarbose Analysis (PDF file)

Acarbose is used to treat type 2 diabetes. A recent study shows efficacy and tolerability of acarbose in patients with type 2 diabetes. By using COSOMSIL Sugar-D, which is designed for saccharide analysis, highresolution separation of Acarbose and its impurities was obtained. Furthermore, COSMOSIL Sugar-D was more durable than the conventional NH2 column.


Analysis of Sugars in Honey and Syrup (PDF file)

Analysis of honey and syrup is an important part of detecting food adulteration. Sugar-D provides good and consistent separation with high durability.


Usage Information

Column5 µm Sugar-D
I.D. (mm)
Washing method
  1. Remove buffer, salts and/or acid from column: wash for 10-15 min. using the mobile phase last used, without buffer, salts or acid. For example, if your mobile phase was 50:50 acetonitrile/20 mmol/l phosphate buffer, wash with 50:50 acetonitrile/water.
  2. Remove adsorbed compounds and fix unstable baselines: Wash with 50:50 acetonitrile/water or up to 100% water.
Storage conditions Wash the column to remove buffer, salts and/or acid.
Then, replace the solvent with 90:10 acetonitrile/water.

Tightly plug the column, and store in a cool and dry place.
Usable pH range pH 2 - 7.5
Max. pressure 20MPa 15MPa
Temperature range The maximum usable temperature is 60°C. However. for regular use, please use at a constant temperature between 20°C and 50°C.
Usable solvents Acetonitrile/water mobile phases are recommended.
Other instructions
  • Buffer concentration is usually sufficient at 0.005 - 0.02 mol/L.
  • Always filter mobile phases using a 0.5 µm filter before use.
  • Methanol is not recommended as a mobile phase.
  • If using a buffer or acid, we recommend using the column only for that mobile phase. Buffers may affect performance even after washing the column.
  • Neutral pH will maximize retention time.



Effects of ciceritol from chickpeas on human colonic microflora and the production of short chain fatty acids by in vitro fermentation
Yun Zhang, Di Su, Jinyan He, Zhuqing Dai, Riaz Asad, Shiyi Ou, Xiaoxiong Zeng
LWT - Food Science and Technology,  79, 2017, 294-299
Effects of pyruvate kinase on the growth of Corynebacterium glutamicum and L-serine accumulation
Xiaomei Zhang, Lianhe Lai, Guoqiang Xu, Xiaojuan Zhang, Jinsong Shi, Zhenghong Xu
Process Biochemistry, 55, 2017, 32-40,
Improving special hydrolysis characterization into Talaromyces thermophilus F1208 xylanase by engineering of N-terminal extension and site-directed mutagenesis in C-terminal
Qin Li, Baoguo Sun, Ke Xiong, Chao Teng, Youqiang Xu, Liangjun Li, Xiuting Li
International Journal of Biological Macromolecules, 96, 2017, 451-458
Enhancement of fructose utilization from sucrose in the cell for improved l-serine production in engineered Corynebacterium glutamicum
Xiaomei Zhang, Liping Yao, Guoqiang Xu, Jiafen Zhu, Xiaojuan Zhang, Jinsong Shi, Zhenghong Xu
Biochemical Engineering Journal, 118, 2017, 113-122
Promoted isomerization of aldoses to ketoses in subcritical aqueous acetonitrile
Da-Ming Gao, Takashi Kobayashi, Shuji Adachi
The Canadian Journal of Chemical Engineering, 95 (2), 2017, 359-363
Bacillus subtilis iolU encodes an additional NADP+- dependent scyllo-inositol dehydrogenase
Dong-Min Kang, Kosei Tanaka, Shinji Takenaka, Shu Ishikawa & Ken-ichi Yoshida
Bioscience, Biotechnology, and Biochemistry, 81 (5), 2017, 1026-1032
Evaluation of the chemical quality of Sekkoku ( 石斛 ) in current Japanese commercial crude drugs: constituents of Flickingeria xantholeuca (Rchb. f.) A.D. Hawkes
Mei Muto, Haruka Asahina, Tatsufumi Yoshioka, Kazuo Ozaki, Makio Shibano
Journal of Natural Medicines, 71 (1), 2017, 238-248
d-Pinitol in Fabaceae: an Oviposition Stimulant for the Common Grass Yellow Butterfly, Eurema mandarina
Shin-ya Mukae, Toshiki Ohashi, Yuika Matsumoto, Shinji Ohta, Hisashi Ômura
Journal of Chemical Ecology, 42 (11), 1122-1129, 2016
Decomposition Kinetics of Glucose and Fructose in Subcritical Water Containing Sodium Chloride
Takashi Kobayashi, Pramote Khuwijitjaru, Shuji Adachi
Journal of Applied Glycoscience, 63 (4), 99-104, 2016
Efficient and environmental-friendly dehydration of fructose to 5-hydroxymethyl-2-furfural in water under high pressure of CO2
Suguru Motokucho, Hiroshi Morikawa, Hisayuki Nakatani, Bart A.J. Noordover
Tetrahedron Letters, 57 (42), 2016, 4742-4745
Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine and melibiose using Aspergillus niger α-galactosidase, and properties of the products
Yohei Sakaki, Mitsuru Tashiro, Moe Katou, Chiseko Sakuma, Takako Hirano, Wataru Hakamata & Toshiyuki Nishio
Bioscience, Biotechnology, and Biochemistry, 80 (9), 2016, 1836-1842
Investigation of the transglycosylation potential of β-Galactosidase from Aspergillus oryzae in the presence of the ionic liquid [Bmim][PF6]
Regina Brakowski, Katrin Pontius, Matthias Franzreb
Journal of Molecular Catalysis B: Enzymatic, 130, 2016, 48-57
Analysis of Dissolved Organic Nutrients in the Interstitial Water of Natural Biofilms
Yuki Tsuchiya, Shima Eda, Chiho Kiriyama, Tomoya Asada, Hisao Morisaki
Microbial Ecology, 72 (1), 2016, 85-95
Physicochemical parameters, bioactive compounds and microbial quality of sonicated pear juice
Muhammad Saeeduddin, Muhammad Abid, Saqib Jabbar, Bing Hu, Malik Muhammad Hashim, Muhammad Ammar Khan, Minhao Xie, Tao Wu, Xiaoxiong Zeng
Food Science + Technology, 51 (7), 2016, 1552-1559

COSMOSIL Application Search

COSMOSIL Application, which includes more than 7,700 applications using COSMOSIL/COSMOCORE columns, is now usable by clicking the link below which is in Nacalai Tesque, Inc. The application is searchable by sample category, sample name, CAS No., column name and particle size

Ordering Information

Product Description Cat.No. PKG Size Price  
COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 50 mm) End-Fitting: Waters type
07974-21 1 pkg 514.00 Buy
COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 100 mm) End-Fitting: Waters type
15378-91 1 pkg 535.00 Buy
COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 150 mm) End-Fitting: Waters type
05688-41 1 pkg 546.00 Buy
COSMOSIL Sugar-D Packed Column (2.0 mm I.D. x 250 mm) End-Fitting: Waters type
05689-31 1 pkg 659.00 Buy
COSMOSIL Sugar-D Packed Column (3.0 mm I.D. x 75 mm) End-Fitting: Waters type
11565-51 1 pkg - Inquire
COSMOSIL Sugar-D Packed Column (3.0 mm I.D. x 150 mm) End-Fitting: Waters type
05690-91 1 pkg 493.00 Buy
COSMOSIL Sugar-D Packed Column (3.0 mm I.D. x 250 mm) End-Fitting: Waters type
05691-81 1 pkg 578.00 Buy
COSMOSIL Sugar-D Packed Column (4.6 mm I.D. x 50 mm) End-Fitting: Waters type
07975-11 1 pkg 428.00 Buy
COSMOSIL Sugar-D Packed Column (4.6 mm I.D. x 150 mm) End-Fitting: Waters type
05395-71 1 pkg 461.00 Buy
COSMOSIL Sugar-D Packed Column (4.6 mm I.D. x 250 mm) End-Fitting: Waters type
05397-51 1 pkg 552.00 Buy
COSMOSIL Sugar-D Packed Column (10.0 mm I.D. x 250 mm) End-Fitting: Waters type
05692-71 1 pkg 1,295.00 Buy
COSMOSIL Sugar-D Packed Column (20.0 mm I.D. x 250 mm) End-Fitting: Waters type
05693-61 1 pkg 2,925.00 Buy
Guard Column / Guard Cartridge
Product Description Cat.No. PKG Size Price  
COSMOSIL Sugar-D Guard Cartridge (4.6 mm I.D. x 10 mm) End-Fitting: Waters type
19185-04 2 pkg 236.00 Buy
COSMOSIL Sugar-D Guard Column (10.0 mm I.D. x 20 mm) End-Fitting: Waters type
05696-31 1 pkg 632.00 Buy
COSMOSIL Sugar-D Guard Column (20.0 mm I.D. x 50 mm) End-Fitting: Waters type
05694-51 1 pkg 1,461.00 Buy
HPLC Accessories
Product Description Cat.No. PKG Size Price  
COSMOSIL Direct Cartridge Holder End-Fitting: Waters type
19989-71 1 pkg 270.00 Buy
COSMOSIL Guard Cartridge Holder (4.6 mm I.D.) **Discontinued** End-Fitting: Waters type
38009-79 1 pkg - Inquire
COSMOSIL Guard Cartridge Holder (2.0 mm I.D.) End-Fitting: Waters type
11884-71 1 pkg 265.00 Buy
COSMOSIL Column Prefilter End-Fitting: Waters type
39361-19 1 pkg 145.00 Buy
COSMOSIL Column Spare Filter for Prefilter (1 micron) End-Fitting: Waters type
39539-09 2 pkg 55.00 Buy
COSMOSIL Column Connecting Tube (0.25 mm) End-Fitting: Waters type
37843-69 1 pkg 65.00 Buy
COSMOSIL Column Connecting Tube (0.1 mm) End-Fitting: Waters type
12570-41 1 pkg 65.00 Buy

#38009-79 has been discontinued. Alternative product is COSMOSIL Direct Cartridge Holder (#19989-71).