Immunohistochemistry
-Histofine® Simple Stain MAX PO is a detection reagent designed specifically to allow immunohistochemical staining on formalin-fixed paraffin-embedded human tissue sections. It is the labeled polymer prepared by combining amino acid polymers with peroxidase (PO) and secondary antibody which is reduced to Fab' fragment. To eliminate background staining, solid-phase absorption of secondary antibody is carried out with human serum.
Selection Guide
Polymer technology
Multiple molecules of enzyme and secondary antibodies conjugate to amino-acid polymers.
Polymeric enzyme provides increased staining intensity.
Simplified staining steps
Low background
Only Fab' fragments are used as secondary antibodies. As Fab' fragment doesn't bind to endogenous Fc receptor, the background remains clean.
Histofine Simple Stain is a Non-biotin detection system.
Comparison between Histofine and conventional SAB System.
Technical Report 1 : IHC Triple-staining Method.
Technical Report 2 : Recommendation of Immunohistochemical polymer detection systems designed for mouse & rat tissue sections.
(These links above will direct you to Nichirei Biosciences site)
Fig. 1 Background comparison between the mouse tissue staining kit and the human tissue staining kit
Fig. 2 Background comparison between the rat tissue staining kit and the human tissue staining kit
Problem | Possible Cause | Solution | |
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[Peroxidase staining] | ||
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Product | Serum | ||
Simple Stain™ MAX PO (M) | Goat | ||
Simple Stain™ MAX PO (R) | Goat | ||
Simple Stain™ MAX PO (MULTI) | Goat | ||
Simple Stain™ MAX PO (G) | Rabbit | ||
Simple Stain™ Mouse MAX PO (R) | Goat | ||
Simple Stain™ Mouse MAX PO (Rat) | Rabbit | ||
Simple Stain™ Rat MAX PO (M) | Goat | ||
Simple Stain™ Rat MAX PO (R) | Goat | ||
Simple Stain™ Rat MAX PO (G) | Rabbit | ||
Simple Stain™ Rat MAX PO (MULTI) | Goat | ||
[Alkaline phosphatase staining] | |||
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Product name | Serum | ||
Simple Stain™ AP (M) | Goat | ||
Simple Stain™ AP (R) | Goat | ||
Simple Stain™ AP (MULTI) | Goat | ||
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Product | Cat.No. | Storage | PKG Size | Price(US$) | |
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Histofine DAB-3S Kit (for Peroxidase) | 415192F | 4°C | 500 tests | 290.00 | Buy |
Histofine DAB-3S Kit (for Peroxidase) | 415194F | 4°C | 1500 tests | 330.00 | Buy |
Histofine DAB-2V Kit (for Peroxidase) | 425312F | 4°C | 500 tests | 210.00 | Buy |
Histofine DAB-2V Kit (for Peroxidase) | 425314F | 4°C | 1500 tests | 260.00 | Buy |
Histofine Simple Stain AEC Solution (for Peroxidase) | 415182F | 4°C | 500 tests | 300.00 | Buy |
Histofine Simple Stain AEC Solution (for Peroxidase) | 415184F | 4°C | 1500 tests | 370.00 | Buy |
Histofine New Fuchsin Substrate Kit (for Alkaline phosphatase) | 415161F | 4°C | 2000 tests | 210.00 | Buy |
Product | Cat.No. | Storage | PKG Size | Price(US$) | |
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Histofine High Stain Human HRP (MULTI) -High Sensitive 2 Step Polymer Detection System | 414481F | 4°C | 170 tests | 640.00 | Buy |
Histofine High Stain Human HRP (MULTI) -High Sensitive 2 Step Polymer Detection System | 414483F | 4°C | 1000 tests | 1,430.00 | Buy |
Histofine Simple Stain Human MAX PO (MULTI) (for Mouse & Rabbit primary antibody) | 414151F | 4°C | 170 tests | 240.00 | Buy |
Histofine Simple Stain Human MAX PO (MULTI) (for Mouse & Rabbit primary antibody) | 414152F | 4°C | 500 tests | 410.00 | Buy |
Histofine Simple Stain Human MAX PO (MULTI) (for Mouse & Rabbit primary antibody) | 414154F | 4°C | 1500 tests | 710.00 | Buy |
Histofine Simple Stain Human MAX PO (M) (for Mouse primary antibody) | 414131F | 4°C | 170 tests | 240.00 | Buy |
Histofine Simple Stain Human MAX PO (M) (for Mouse primary antibody) | 414132F | 4°C | 500 tests | 400.00 | Buy |
Histofine Simple Stain Human MAX PO (M) (for Mouse primary antibody) | 414134F | 4°C | 1500 tests | 680.00 | Buy |
Histofine Simple Stain Human MAX PO (R) (for Rabbit primary antibody) | 414141F | 4°C | 170 tests | 240.00 | Buy |
Histofine Simple Stain Human MAX PO (R) (for Rabbit primary antibody) | 414142F | 4°C | 500 tests | 400.00 | Buy |
Histofine Simple Stain Human MAX PO (R) (for Rabbit primary antibody) | 414144F | 4°C | 1500 tests | 680.00 | Buy |
Histofine Simple Stain Human MAX PO (G) (for Goat primary antibody) | 414161F | 4°C | 170 tests | 260.00 | Buy |
Histofine Simple Stain Human MAX PO (G) (for Goat primary antibody) | 414162F | 4°C | 500 tests | 390.00 | Buy |
Histofine Simple Stain Human AP (MULTI) (for Mouse & Rabbit primary antibody) | 414261F | 4°C | 170 tests | 300.00 | Buy |
Histofine Simple Stain Human AP (MULTI) (for Mouse & Rabbit primary antibody) | 414262F | 4°C | 500 tests | 640.00 | Buy |
Histofine Simple Stain Human AP (M) (for Mouse primary antibody) | 414241F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Human AP (M) (for Mouse primary antibody) | 414242F | 4°C | 500 tests | 630.00 | Buy |
Histofine Simple Stain Human AP (R) (for Rabbit primary antibody) | 414251F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Human AP (R) (for Rabbit primary antibody) | 414252F | 4°C | 500 tests | 630.00 | Buy |
Product | Cat.No. | Storage | PKG Size | Price(US$) | |
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Histofine ALK Detection Kit | 417071F | 4°C | 20 tests | 860.00 | Buy |
Histofine ALK Control Slides | 417081F | 4°C | 5 slides | 500.00 | Buy |
Product | Cat.No. | Storage | PKG Size | Price(US$) | |
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Histofine Simple Stain MOUSESTAIN KIT | 414321F | 4°C | 50 tests | 400.00 | Buy |
Histofine Simple Stain MOUSESTAIN KIT | 414322F | 4°C | 500 tests | 700.00 | Buy |
Histofine Simple Stain Mouse MAX PO (R) (for Rabbit primary antibody) | 414341F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Mouse MAX PO (G) (for Goat primary antibody) | 414351F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Mouse MAX PO (Rat) (for Rat primary antibody) | 414311F | 4°C | 170 tests | 270.00 | Buy |
Product | Cat.No. | Storage | PKG Size | Price(US$) | |
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Histofine Simple Stain Rat MAX PO (MULTI) (for Mouse & Rabbit primary antibody) | 414191F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Rat MAX PO (M) (for Mouse primary antibody) | 414171F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Rat MAX PO (R) (for Rabbit primary antibody) | 414181F | 4°C | 170 tests | 270.00 | Buy |
Histofine Simple Stain Rat MAX PO (G) (for Goat primary antibody) | 414331F | 4°C | 170 tests | 270.00 | Buy |