Blocking Reagent: Blocking One Histo
for Immunohistochemistry Application
Blocking One Histo is a blocking buffer to prevent non-specific binding of antibodies in immunohistochemistry (IHC).
Easy-to-use: eye-drop bottle
Confirmed: Can be used for immunofluorescence staining
Safe: the preservative does not affect the activity of alkaline phosphatase or horseradish peroxidase.
Comparison of blocking efficient with 10% Goat Serum (Immunofluorescence)
In both panels, mouse small intestine tissue section was stained with secondary antibody conjugated with CF™ 488A (green) and counter stained with DAPI (blue). In the panel B with 10% Goat Serum, the stained white arrow along the lines of shape of small intestine show non-specific staining. Blocking One Histo is more effective at reducing non-specific background staining than normal serum.
Application data
| A: Mouse small intestine | (PCNA) x5 |
| B: Mouse epididymis | (Vimentin) x25 |
| C: Mouse brain | (GluR) x100 |
Blocking treatment of each tissue section had been performed by Blocking One Histo. Mouse small intestine (panel A) was stained with anti-PCNA and DAB (3,3'-Diamino Benzidine) to stain nuclear (black arrow), Mouse epididymis (panel B) was stained with anti-Vimentin and DAB to stain nuscle (black arrow), Mouse brain (panel C) was stained with anti-GluR and DAB to stain membrane proteins (black arrow) and counter stained with hematoxylin.
Ordering Information
| Product | Cat.No. | Storage | Quantity | Price(US$) | |
|---|---|---|---|---|---|
| Blocking One Histo | 06349-64 | 2-8°C | 50 ml | 100.00 | Buy |