for Immunohistochemistry Application
Blocking One Histo is a blocking buffer to prevent non-specific binding of antibodies in immunohistochemistry (IHC).
In both panels, mouse small intestine tissue section was stained with secondary antibody conjugated with CF™ 488A (green) and counter stained with DAPI (blue). In the panel B with 10% Goat Serum, the stained white arrow along the lines of shape of small intestine show non-specific staining. Blocking One Histo is more effective at reducing non-specific background staining than normal serum.
|A: Mouse small intestine||(PCNA) x5|
|B: Mouse epididymis||(Vimentin) x25|
|C: Mouse brain||(GluR) x100|
Blocking treatment of each tissue section had been performed by Blocking One Histo. Mouse small intestine (panel A) was stained with anti-PCNA and DAB (3,3'-Diamino Benzidine) to stain nuclear (black arrow), Mouse epididymis (panel B) was stained with anti-Vimentin and DAB to stain nuscle (black arrow), Mouse brain (panel C) was stained with anti-GluR and DAB to stain membrane proteins (black arrow) and counter stained with hematoxylin.
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|Blocking One Histo||06349-64||2-8°C||50 ml||100.00||Buy|