Blocking Reagent: Blocking One Histo
for Immunohistochemistry Application
Blocking One Histo is a blocking buffer to prevent non-specific binding of antibodies in immunohistochemistry (IHC).
Easy-to-use: eye-drop bottle
Confirmed: Can be used for immunofluorescence staining
Safe: the preservative does not affect the activity of alkaline phosphatase or horseradish peroxidase.
Comparison of blocking efficient with 10% Goat Serum (Immunofluorescence)
In both panels, mouse small intestine tissue section was stained with secondary antibody conjugated with CF™ 488A (green) and counter stained with DAPI (blue). In the panel B with 10% Goat Serum, the stained white arrow along the lines of shape of small intestine show non-specific staining. Blocking One Histo is more effective at reducing non-specific background staining than normal serum.
Application data
| A: Mouse small intestine | (PCNA) x5 |
| B: Mouse epididymis | (Vimentin) x25 |
| C: Mouse brain | (GluR) x100 |
Blocking treatment of each tissue section had been performed by Blocking One Histo. Mouse small intestine (panel A) was stained with anti-PCNA and DAB (3,3'-Diamino Benzidine) to stain nuclear (black arrow), Mouse epididymis (panel B) was stained with anti-Vimentin and DAB to stain nuscle (black arrow), Mouse brain (panel C) was stained with anti-GluR and DAB to stain membrane proteins (black arrow) and counter stained with hematoxylin.
Reference
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- Shibuya, Shuichi, et al. "Acai Extract Transiently Upregulates Erythropoietin by Inducing a Renal Hypoxic Condition in Mice." Nutrients 12.2 (2020): 533.
- Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.
- Kugo, Hirona, Tatsuya Moriyama, and Nobuhiro Zaima. "The role of perivascular adipose tissue in the appearance of ectopic adipocytes in the abdominal aortic aneurysmal wall." Adipocyte 8.1 (2019): 229-239.
- Kitagawa, Koichi, et al. "Possible correlation of sonic hedgehog signaling with epithelial–mesenchymal transition in muscle-invasive bladder cancer progression." Journal of cancer research and clinical oncology 145.9 (2019): 2261-2271.
- Shoji, Tetsuaki, et al. "Evaluating the immunoproteasome as a potential therapeutic target in cisplatin-resistant small cell and non-small cell lung cancer." Cancer chemotherapy and pharmacology (2020).
- Omotehara, Takuya, et al. "Connection between seminiferous tubules and epididymal duct is originally induced before sex differentiation in a sex‐independent manner." Developmental Dynamics (2020).
- Moro, Hiroshi, et al. "Epigenetic priming sensitizes gastric cancer cells to irinotecan and cisplatin by restoring multiple pathways." Gastric cancer 23.1 (2020): 105-115.
Ordering Information
| Product | Cat.No. | Storage | PKG Size | Price(US$) | |
|---|---|---|---|---|---|
| Blocking One Histo | 06349-64 | 2-8°C | 50 ml | 100.00 | Buy |