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Blocking Reagent: Blocking One Histo

for Immunohistochemistry Application

Blocking One Histo is a blocking buffer to prevent non-specific binding of antibodies in immunohistochemistry (IHC).

Easy-to-use: eye-drop bottle

Confirmed: Can be used for immunofluorescence staining

Safe: the preservative does not affect the activity of alkaline phosphatase or horseradish peroxidase.

Comparison of blocking efficient with 10% Goat Serum (Immunofluorescence)

In both panels, mouse small intestine tissue section was stained with secondary antibody conjugated with CF™ 488A (green) and counter stained with DAPI (blue). In the panel B with 10% Goat Serum, the stained white arrow along the lines of shape of small intestine show non-specific staining. Blocking One Histo is more effective at reducing non-specific background staining than normal serum.

Application data

A: Mouse small intestine (PCNA) x5
B: Mouse epididymis (Vimentin) x25
C: Mouse brain (GluR) x100

Blocking treatment of each tissue section had been performed by Blocking One Histo. Mouse small intestine (panel A) was stained with anti-PCNA and DAB (3,3'-Diamino Benzidine) to stain nuclear (black arrow), Mouse epididymis (panel B) was stained with anti-Vimentin and DAB to stain nuscle (black arrow), Mouse brain (panel C) was stained with anti-GluR and DAB to stain membrane proteins (black arrow) and counter stained with hematoxylin.

Reference

  1. Takahashi, Haruka, et al. "Establishment and characterization of human gingival squamous cell carcinoma cell line NOCS-1." Human cell 32.1 (2019): 75-82.
  2. Shibuya, Shuichi, et al. "Acai Extract Transiently Upregulates Erythropoietin by Inducing a Renal Hypoxic Condition in Mice." Nutrients 12.2 (2020): 533.
  3. Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.
  4. Kugo, Hirona, Tatsuya Moriyama, and Nobuhiro Zaima. "The role of perivascular adipose tissue in the appearance of ectopic adipocytes in the abdominal aortic aneurysmal wall." Adipocyte 8.1 (2019): 229-239.
  5. Kitagawa, Koichi, et al. "Possible correlation of sonic hedgehog signaling with epithelial–mesenchymal transition in muscle-invasive bladder cancer progression." Journal of cancer research and clinical oncology 145.9 (2019): 2261-2271.
  6. Shoji, Tetsuaki, et al. "Evaluating the immunoproteasome as a potential therapeutic target in cisplatin-resistant small cell and non-small cell lung cancer." Cancer chemotherapy and pharmacology (2020).
  7. Omotehara, Takuya, et al. "Connection between seminiferous tubules and epididymal duct is originally induced before sex differentiation in a sex‚Äźindependent manner." Developmental Dynamics (2020).
  8. Moro, Hiroshi, et al. "Epigenetic priming sensitizes gastric cancer cells to irinotecan and cisplatin by restoring multiple pathways." Gastric cancer 23.1 (2020): 105-115.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Blocking One Histo 06349-64 2-8°C 50 ml 100.00 Buy