This product leads to effective elution of antibodies from protein A columns under mild pH (pH 4.0). Normally, antibodies are not effectively eluted at pH 4.0. Use of a lower pH can lead to partial denaturation and subsequent aggregation of the eluted antibodies. This product is based on the unique characteristics of arginine, which suppresses protein-protein interactions.
This product is manufactured with permission from Ajinomoto Co., Inc. based on the patent US 8084032, 8435527, 2012-0264918*.
*JP: 4826995, US: 8084032, 8435527, 2012-0264918, EP: 1568710, CN: 1680426
Arg-Antibody elution buffer shows much higher recovery (lane 6) than glycine buffer (lane 5).
Lane 1: Molecular weight marker (#09547-74)
Lane 2: Serum
Lane 3: 0.1 M Gly pH 2.8
Lane 4: 0.1 M Gly pH 3.4
Lane 5: 0.1 M Gly pH 4.0
Lane 6: Arg-Antibody Elution Buffer
Note that lanes 3, 4, 5 and 6 are independent results of the above procedure using
different elution buffers.
Purification of antibody on Protein A column
Cation exchange chromatography of the above eluted sample
As Arg-Antibody Elution Buffer can effectively weaken protein-protein interactions, there are a few additional applications of this product.
Elution of antibodies from Protein-A column by aqueous arginine solutions. Protein Expression and Purification 36(2), 244-248 (2004).
Effective elution of antibodies by arginine and arginine derivatives in affinity column chromatography. Analytical Biochemistry 345(2), 250-257 (2005).
Role of arginine in protein refolding, solubilization, and purification. Biotechnology Progress 20(5), 1301-1308 (2004).
Screening of effective column rinse solvent for Protein-A chromatography. Protein Expression and Purification 70(2),218-223 (2010).