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RIPA Buffer

Cell Lysis Solution

RIPA Buffer is a ready-to-use solution containing a variety of surfactants and protease inhibitors. Proteins lysed with RIPA Buffer can be used in western blotting, ELISA or immunoprecipitation testing regimes.

  • Ready-to-use
  • Contains protease inhibitors
  • Unmixes SDS solution for immunoprecipitation
  • Applicable BCA protein assay without buffer exchange

 

Product Detail

Product Name RIPA Buffer RIPA Buffer (10x)
Cat. No. 16488-34 08714-04
Concentrataion 1x (ready to use) 10x
Protease inhibitor Cocktail with without
Composition
  • 50mmol/l Tris-HCl buffer(pH7.6)
  • 150mmol/l NaCl
  • 1% Nonidet® P40
  • 0.5% Sodium Deoxycholate
  • 0.1% SDS
【1x solution with SDS】
  • 50mmol/l Tris-HCl buffer(pH7.6)
  • 150mmol/l NaCl
  • 1% Nonidet® P40
  • 0.5% Sodium Deoxycholate
  • Protease Inhibitor Cocktail(1×)
  • (0.1% SDS)
Volume /  Content 100mL
  • RIPA Buffer(10x): 2ml×5本
  • SDS Solution(1% SDS): 2ml×5本

 

 

Data

Electrophoresis

Electrophoresis_RIPA

 

Immunoprecipitation and Western Blot

Immunoprecipitation_RIPA

 

Western Blot

WB_RIPA

 

Reference

  1. Hagiwara, Asami, et al. "Induction of tryptophan hydroxylase in the liver of sc tumor model of prostate cancer." Cancer Science 111.4 (2020): 1218.
  2. Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.
  3. Tsukamoto, Sho, Mai Kuratani, and Takenobu Katagiri. "Functional characterization of a unique mutant of ALK2, p. K400E, that is associated with a skeletal disorder, diffuse idiopathic skeletal hyperostosis." Bone (2020): 115410.
  4. Thapa, Jeewan, et al. "Hypoxia promotes Chlamydia trachomatis L2/434/Bu growth in immortal human epithelial cells via activation of the PI3K-AKT pathway and maintenance of a balanced NAD+/NADH ratio." Microbes and Infection (2020).
  5. Tomoyasu, Chihiro, et al. "OBP‑801, a novel histone deacetylase inhibitor, induces M‑phase arrest and apoptosis in rhabdomyosarcoma cells." Oncology reports 41.1 (2019): 643-649.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
RIPA Buffer (10X) 08714-04 -20 °C 1 set 129.00 Buy
RIPA Buffer 16488-34 4 °C 100 ml 92.00 Buy