RIPA Buffer
Cell Lysis Solution
RIPA Buffer is a ready-to-use solution containing a variety of surfactants and protease inhibitors. Proteins lysed with RIPA Buffer can be used in western blotting, ELISA or immunoprecipitation testing regimes.
- Ready-to-use
- Contains protease inhibitors
- Unmixes SDS solution for immunoprecipitation
- Applicable BCA protein assay without buffer exchange
Data
Electrophoresis
Immunoprecipitation and Western Blot
Western Blot
RIPA Buffer (10X) (Instruction)Reference
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Hagiwara, Asami, et al. "Induction of tryptophan hydroxylase in the liver of sc tumor model of prostate cancer." Cancer Science 111.4 (2020): 1218.
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Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.
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Tsukamoto, Sho, Mai Kuratani, and Takenobu Katagiri. "Functional characterization of a unique mutant of ALK2, p. K400E, that is associated with a skeletal disorder, diffuse idiopathic skeletal hyperostosis." Bone (2020): 115410.
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Thapa, Jeewan, et al. "Hypoxia promotes Chlamydia trachomatis L2/434/Bu growth in immortal human epithelial cells via activation of the PI3K-AKT pathway and maintenance of a balanced NAD+/NADH ratio." Microbes and Infection (2020).
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Tomoyasu, Chihiro, et al. "OBP‑801, a novel histone deacetylase inhibitor, induces M‑phase arrest and apoptosis in rhabdomyosarcoma cells." Oncology reports 41.1 (2019): 643-649.
Product Detail
| Product Name | RIPA Buffer | RIPA Buffer (10x) |
| Cat. No. | 16488-34 | 08714-04 |
| Concentrataion | 1x (ready to use) | 10x |
| Protease inhibitor Cocktail | without | with |
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Composition
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【1x solution with SDS】
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Volume / Content
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100 mL
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