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RIPA Buffer

Cell Lysis Solution

RIPA Buffer is a ready-to-use solution containing a variety of surfactants and protease inhibitors. Proteins lysed with RIPA Buffer can be used in western blotting, ELISA or immunoprecipitation testing regimes.

  • Ready-to-use
  • Contains protease inhibitors
  • Unmixes SDS solution for immunoprecipitation
  • Applicable BCA protein assay without buffer exchange






Immunoprecipitation and Western Blot



Western Blot




  1. Hagiwara, Asami, et al. "Induction of tryptophan hydroxylase in the liver of sc tumor model of prostate cancer." Cancer Science 111.4 (2020): 1218.

  2. Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.

  3. Tsukamoto, Sho, Mai Kuratani, and Takenobu Katagiri. "Functional characterization of a unique mutant of ALK2, p. K400E, that is associated with a skeletal disorder, diffuse idiopathic skeletal hyperostosis." Bone (2020): 115410.

  4. Thapa, Jeewan, et al. "Hypoxia promotes Chlamydia trachomatis L2/434/Bu growth in immortal human epithelial cells via activation of the PI3K-AKT pathway and maintenance of a balanced NAD+/NADH ratio." Microbes and Infection (2020).

  5. Tomoyasu, Chihiro, et al. "OBP‑801, a novel histone deacetylase inhibitor, induces M‑phase arrest and apoptosis in rhabdomyosarcoma cells." Oncology reports 41.1 (2019): 643-649.

Product Detail

Product Name RIPA Buffer RIPA Buffer (10x)
Cat. No. 16488-34 08714-04
Concentrataion 1x (ready to use) 10x
Protease inhibitor Cocktail without with
  • 50mmol/l Tris-HCl buffer(pH7.6)
  • 150mmol/l NaCl
  • 1% Nonidet® P40
  • 0.5% Sodium Deoxycholate
  • 0.1% SDS
【1x solution with SDS】
  • 50mmol/l Tris-HCl buffer(pH7.6)
  • 150mmol/l NaCl
  • 1% Nonidet® P40
  • 0.5% Sodium Deoxycholate
  • Protease Inhibitor Cocktail(1×)
  • (0.1% SDS)
Volume /  Content
100 mL
  • RIPA Buffer(10x): 2ml×5本

  • SDS Solution(1% SDS): 2ml×5本



Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
RIPA Buffer (10X) 08714-04 -20 °C 1 set 129.00 Buy
RIPA Buffer 16488-34 4 °C 100 ml 92.00 Buy