Protease Inhibitor Cocktail for Use with Mammalian Cell and Tissue Extracts
for Mammalian Cell and Tissue Extracts
- The product contain 6 kinds of protease inhibitors for different targets.
- The product is a concentrated type. Dilute with any buffer of your choice.
Composition
The protease inhibitors are mixed in Dimethyl sulfoxide (DMSO)
| Inhibitors | Targeted proteasee |
|---|---|
| 100 mmol/l 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) | Serine protease |
| 80 µmol/l Aprotinin from bovine lung | Serine protease, elastase |
| 1.5 mmol/l E-64 | Cystein protease |
| 2 mmol/l Leupeptin hemisulfate monohydrate | Cystein protease, trypsin like protease |
| 5 mmol/l Bestatin | Aminopeptidase |
| 1 mmol/l Pepstatin A | Asparticprotease |
Protocol
Dilute 50 - 100 times with any buffer of your choice
Protease Inhibitor Cocktail for Use with Mammalian Cell and Tissue Extracts (Instruction)Reference
Note
In case of very high protease concentration, use higher concentration of the product