Phosphatase Inhibitor Cocktail
Phosphatase Inhibitor Cocktail is a mixture of several inhibitors to protect valuable proteins from dephosphorylation. The product preserves phosphorylated proteins existing in small quantity in cells and tissues.
Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)
- Contains 7 kinds of phosphatase inhibitors for different targets.
- 100 times concentrated stock solution. Dilute with any buffer of your choice.
- Stable for two years in refrigerator (2-8 °C).
- Ready to use.
Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)
This product does not interfere with metalloproteins since it is EDTA free.
- Contains 6 kinds of phosphatase inhibitors for different targets.
- 100 times concentrated stock solution. Dilute with any buffer of your choice.
- Stable for two years in refrigerator (2-8 °C).
- Ready to use.
Comparison data
The product inhibits Ser/Thr phosphatase, Tyrosine Phosphatase, Acid Phosphatase and Alkaline Phosphatase. The product preserves small quantity of phosphorylated protein.
Composition
Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)
The product (100 times concentrated stock solution) contains:
| Inhibitors | Targeted phosphatase |
|---|---|
| Imidazole | Alkaline Phosphatase |
| Sodium Orthovanadate (V) | Protein Tyrosine Phosphatase Alkaline Phosphatase |
| b-Glycerophosphoric Acid Disodium Salt | Ser/Ther Phosphatase |
| Sodium (+)-Tartrate Dihydrate | Acid Phosphatase |
| Sodium Fluoride | Acid Phosphatase Protein Phosphatase-2A Protein Phosphatase-2B Protein Phosphatase-2C |
| Disodium Molybdate (VI) Dihydrate | Acid Phosphatase Phosphoprotein phosphatase |
Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)
The product (100 times concentrated stock solution) contains:
| Inhibitors | Targeted phosphatase |
|---|---|
| Imidazole | Alkaline Phosphatase |
| tetra-Sodium Ethylenediaminetetraacetate | Alkaline Phosphatase Protein Phosphatase-2B Protein Phosphatase-2C |
| Sodium Orthovanadate (V) | Protein Tyrosine Phosphatase Alkaline Phosphatase |
| β-Glycerophosphoric Acid Disodium Salt | Ser/Thr Phosphatase |
| Sodium (+)-Tartrate Dihydrate | Acid Phosphatase |
| Sodium Fluoride | Acid Phosphatase Protein Phosphatase-2A Protein Phosphatase-2B Protein Phosphatase-2C |
| Disodium Molybdate(VI) Dihydrate | Acid Phosphatase Phosphoprotein phosphatase |
Protocol
Downloads
Phosphatase Inhibitor Cocktail (InstructionReference
Note
Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)
- In case of very high phosphatase concentration, use higher concentration of the product (dilute from 5 to 20 times).
- The product may cause inhibition of metal chelate affinity chromatography and 2D electrophoresis. Remove inhibitors by dialysis or desalting before performing such procedures.
- The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (Code No.: 25955-11, Code No.: 03969-21 and Code No.: 04080-11).
Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)
- If phosphatase concentration is high, it can be diluted by 20-50 fold.
- The product may cause inhibition of 2D electrophoresis. Remove inhibitors by dialysis or desalt before use.
- The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (EDTA free) (Cat. No.: 03969).
- The product includes Imidazole for inhibitor. In case of Metal Affinity Purification, it is recommended a pretest with reducing Imidazole concentration in the buffer by 5 mM.
- The product colors gradually.
- Direct light may interfere with an inhibition effect; shield the product from the light after using.
- The components may be precipitated during storage. Before use, melt it by vortex at 30-40°C shielding light.