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Phosphatase Inhibitor Cocktail

Phosphatase Inhibitor Cocktail is a mixture of several inhibitors to protect valuable proteins from dephosphorylation. The product preserves phosphorylated proteins existing in small quantity in cells and tissues.

 

Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)

  • Contains 7 kinds of phosphatase inhibitors for different targets.
  • 100 times concentrated stock solution. Dilute with any buffer of your choice.
  • Stable for two years in refrigerator (2-8 °C).
  • Ready to use.

 

Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)

This product does not interfere with metalloproteins since it is EDTA free.

  • Contains 6 kinds of phosphatase inhibitors for different targets.
  • 100 times concentrated stock solution. Dilute with any buffer of your choice.
  • Stable for two years in refrigerator (2-8 °C).
  • Ready to use.

Comparison data

The product inhibits Ser/Thr phosphatase, Tyrosine Phosphatase, Acid Phosphatase and Alkaline Phosphatase. The product preserves small quantity of phosphorylated protein.

Composition

Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)

 

The product (100 times concentrated stock solution) contains:

InhibitorsTargeted phosphatase
Imidazole Alkaline Phosphatase
Sodium Orthovanadate (V) Protein Tyrosine Phosphatase
Alkaline Phosphatase
b-Glycerophosphoric Acid Disodium Salt Ser/Ther Phosphatase
Sodium (+)-Tartrate Dihydrate Acid Phosphatase
Sodium Fluoride Acid Phosphatase
Protein Phosphatase-2A
Protein Phosphatase-2B
Protein Phosphatase-2C
Disodium Molybdate (VI) Dihydrate Acid Phosphatase
Phosphoprotein phosphatase

 

Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)

The product (100 times concentrated stock solution) contains:

InhibitorsTargeted phosphatase
Imidazole Alkaline Phosphatase
tetra-Sodium Ethylenediaminetetraacetate Alkaline Phosphatase
Protein Phosphatase-2B
Protein Phosphatase-2C
Sodium Orthovanadate (V) Protein Tyrosine Phosphatase
Alkaline Phosphatase
β-Glycerophosphoric Acid Disodium Salt Ser/Thr Phosphatase
Sodium (+)-Tartrate Dihydrate Acid Phosphatase
Sodium Fluoride Acid Phosphatase
Protein Phosphatase-2A
Protein Phosphatase-2B
Protein Phosphatase-2C
Disodium Molybdate(VI) Dihydrate Acid Phosphatase
Phosphoprotein phosphatase

Protocol

Dilute 100 times with any buffer of your choice.

Note

Phosphatase Inhibitor Cocktail (Cat. No.: 07574-61)

  • In case of very high phosphatase concentration, use higher concentration of the product (dilute from 5 to 20 times).
  • The product may cause inhibition of metal chelate affinity chromatography and 2D electrophoresis. Remove inhibitors by dialysis or desalting before performing such procedures.
  • The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (Code No.: 25955-11, Code No.: 03969-21 and Code No.: 04080-11).

Phosphatase Inhibitor Cocktail (EDTA free) (100X) (Cat. No.: 07575-51)

  • If phosphatase concentration is high, it can be diluted by 20-50 fold.
  • The product may cause inhibition of 2D electrophoresis. Remove inhibitors by dialysis or desalt before use.
  • The product does not contain protease inhibitors. For homogenizing cells and tissues, if necessary, add protease inhibitor cocktail, such as Protease Inhibitor Cocktail (EDTA free) (Cat. No.: 03969).
  • The product includes Imidazole for inhibitor. In case of Metal Affinity Purification, it is recommended a pretest with reducing Imidazole concentration in the buffer by 5 mM.
  • The product colors gradually.
  • Direct light may interfere with an inhibition effect; shield the product from the light after using.
  • The components may be precipitated during storage. Before use, melt it by vortex at 30-40°C shielding light.

Ordering Information

Product Cat.No. Storage PKG Size Price(US$)  
Phosphatase Inhibitor Cocktail 07574-61 2-8 °C 1 ml 129.00 Buy
Phosphatase Inhibitor Cocktail (EDTA free) (100X) 07575-51 2-8 °C 1 ml 129.00 Buy