RIPA Buffer
Cell Lysis Solution
RIPA Buffer is a ready-to-use solution containing a variety of surfactants and protease inhibitors. Proteins lysed with RIPA Buffer can be used in western blotting, ELISA or immunoprecipitation testing regimes.
- Ready-to-use
- Contains protease inhibitors
- Unmixes SDS solution for immunoprecipitation
- Applicable BCA protein assay without buffer exchange
Product Detail
| Product Name | RIPA Buffer | RIPA Buffer (10x) |
| Cat. No. | 16488-34 | 08714-04 |
| Concentrataion | 1x (ready to use) | 10x |
| Protease inhibitor Cocktail | with | without |
| Composition |
|
【1x solution with SDS】
|
| Volume / Content | 100mL |
|
Data
Electrophoresis
Immunoprecipitation and Western Blot
Western Blot
Reference
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- Masuda, Takahito, et al. "Anti-HER2 antibody therapy using gene-transduced adipocytes for HER2-positive breast cancer." Breast Cancer Research and Treatment (2020): 1-10.
- Tsukamoto, Sho, Mai Kuratani, and Takenobu Katagiri. "Functional characterization of a unique mutant of ALK2, p. K400E, that is associated with a skeletal disorder, diffuse idiopathic skeletal hyperostosis." Bone (2020): 115410.
- Thapa, Jeewan, et al. "Hypoxia promotes Chlamydia trachomatis L2/434/Bu growth in immortal human epithelial cells via activation of the PI3K-AKT pathway and maintenance of a balanced NAD+/NADH ratio." Microbes and Infection (2020).
- Tomoyasu, Chihiro, et al. "OBP‑801, a novel histone deacetylase inhibitor, induces M‑phase arrest and apoptosis in rhabdomyosarcoma cells." Oncology reports 41.1 (2019): 643-649.