RIPA Buffer
Cell Lysis Solution
RIPA Buffer is a ready-to-use solution containing a variety of surfactants and protease inhibitors. Proteins lysed with RIPA Buffer can be used in western blotting, ELISA or immunoprecipitation testing regimes.
- Ready-to-use
- Contains protease inhibitors
- Unmixes SDS solution for immunoprecipitation
- Applicable BCA protein assay without buffer exchange
Data
Electrophoresis
Immunoprecipitation and Western Blot
Western Blot
Product Detail
| Product Name | RIPA Buffer | RIPA Buffer (10X) |
| Cat. No. | 16488-34 | 08714-04 |
| Concentrataion | 1X (Ready to Use) | 10X |
| Protease inhibitor Cocktail | without | with |
|
Composition
|
- 50 mmol/l Tris-HCl buffer（pH7.6）
- 150 mmol/l NaCl
- 1% Nonidet® P40 Substitute
- 0.5% Sodium Deoxycholate
- 0.1% SDS
|
1X Solution
- 50 mmol/l Tris-HCl buffer（pH7.6）
- 150 mmol/l NaCl
- 1% Nonidet® P40 Substitute
- 0.5% Sodium Deoxycholate
- Protease Inhibitor Cocktail（1X）
- (0.1% SDS)
|
|
Volume / Content
|
- 100 ml x 1 bottle
|
- RIPA Buffer (10X) : 2 ml x 5 bottles
- SDS Solution (1% SDS) : 2 ml x 5 bottle
|
RIPA Buffer (10X) (Instruction)