Protein Ladder (15 - 180 kDa)
The Prestained Protein Ladder, Mid Range is a three-color protein standard with 9 pre-stained proteins covering a wide range molecular weights from 15 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (two green bands at 15 kDa and 60 kDa, and two red bands at 30 kDa and 70 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The Prestained Protein Ladder, Midi Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
Approximately 0.2-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Stable for up to 2 weeks at 25°C
The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
|Prestained Protein Ladder, Mid Range||NU01009||-20°C||500 µl||100.00||Buy|