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SUMO Protease

Tag Removal Protease / Recombinant SUMO Protease (Ready to Use)

SUMO Protease otherwise known as Ulp1, is a recombinant fragment of ULP1 (Ubl-specific protease 1) from Saccharomyces cerevisiae. This highly specific protease cleaves off sumo tags by recognizing the tertiary structure of SUMO as opposed to recognizing its amino acid sequence. Because of its recognition of SUMO’s tertiary structure, it has little to no non-specific proteolysis keeping your protein samples safe after all affinity tags have been cleaved off. SUMO protease is active from 2°C to 37°C in a pH range of 7.0 – 9.0, for lower temperatures allow more time for the cleavage reaction.

 

Synonym(s) :
ULP1, Ulp, Ubl-specific protease 1
Molecular Weight:
27.4 kDa
Purity :
Greater than 90% by SDS-PAGE
Tag :
N-terminal His-tag
Source: 
E. coli
GeneBank Accession # :
QHB12236.1
Activity : 
≥3,000 units/mg protein
Unit Definition :
One unit of SUMO protease cleaves >85% of 3 µg of control substrate in one hour at pH 8.0 at 30 °C.
Application ;
Recombinant protein SUMO tag removal, recombinant protein characterization, ELISA, Western blot, crystallization studies.
Formulation : 
20 mM Tris-HCl, pH8.0, 350 mM NaCl, 10% Glycerol, 1mM 2-Mercaptoethanol
Storage : 
Stable for 12 months at -80°C, Avoid freeze/thaw cycles
Reference :  Elmore, Z.C., et al., BMC Biology 9, 74 (2011).

Ordering Information

Product Cat.No. Purity Storage PKG Size Price(US$)  
Recombinant SUMO Protease (ULP1, Ulp, Ubl-specific protease 1) NU03049-1 90 % or more (SDS-PAGE) -80°C 1,000 units 99.00 Buy
Recombinant SUMO Protease (ULP1, Ulp, Ubl-specific protease 1) NU03049-2 90 % or more (SDS-PAGE) -80°C 10,000 units 699.00 Buy