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Recombinant YopH (Catalytic domain, a.a. 163-468)

The PTP catalytic domain (a.a. 163 to a.a. 468) of Yersinia enterocolitica YopH is expressed in E.coli. The tyrosine-protein phosphatase YopH selectively hydrolyzes only phosphortyrosine-containing proteins and contains a Cys residue essential for catalysis.

 

Synonym(s) : Yop51, Tyrosine-protein phosphatase
Molecular weight : 59 kDa
Purity :
>90% by SDS-PAGE 
Tag : N-terminal GST tag
Source :  E. coli
GenBank Accession # : P08538.3
Application : Remove phosphate group from phosphorated proteins, Phosphatase assay, ELISA, WB, SDS-PAGE, Immunogen, etc
Formulation :  50mM Tris-HCl, 200mM NaCl, 10% Glycerol, pH 8.0
Storage :  Stable for 12 months at -80°C, Avoid freeze/thaw cycles
Reference :  Zhang, Z.Y., et al., The Journal of Biological Chemistry, Vol. 267, No.33, 23759-23766 (1992).

Assay Condition

The enzymatic reactions are performed at 10 µM DiFMUP, indicated amount of Mpro enzyme in 1X assay buffer at room temperature for 30 minutes. The fluorescence intensity is measured at exc/em 360 nm/460 nm.

Ordering Information

Product Cat.No. Purity Storage PKG Size Price(US$)  
Recombinant YopH (Catalytic domain, a.a. 163-468) NU03050-1 90 % or more (SDS-PAGE) -80°C 10 µg 399.00 Buy
Recombinant YopH (Catalytic domain, a.a. 163-468) NU03050-2 90 % or more (SDS-PAGE) -80°C 20 µg 750.00 Buy
Recombinant YopH (Catalytic domain, a.a. 163-468) NU03050-3 90 % or more (SDS-PAGE) -80°C 100 µg 999.00 Buy
Recombinant YopH (Catalytic domain, a.a. 163-468) NU03050-4 90 % or more (SDS-PAGE) -80°C 1 mg 2,199.00 Buy